Microbial analysis of coal polluted soils in the region of Karagandy, Kazakhstan
DOI:
https://doi.org/10.1901/kbs1282Кілттік сөздер:
Coal-contaminated soil, forest soil, pine wood soil, bacterial diversity, biodegradationАннотация
Microorganisms play a major role in ecological biodegradation processes of soil polluted by coal. Here, we assessed the bacterial diversity in coal-contaminated soil samples .The soils samples were taken from four areas for analyses. In present study we conducted the isolation and characterization of microorganisms from Baizhanov mine soil of Karagandy (KCS), State Natural Forest Reserve “Semey Ormany” pinewood soil (SPS), Dmitrievsky forest soil (DFS) and Rudny Altai pinewood soil (APS) .
Twenty-one species of bacteria were isolated from KCS, SPS, DFS and APS soil samples using serial dilution and spread-plate method. Preliminary identification of microorganisms was carried out by culturing on nutrient agar media and Gram staining. Results of total microbial count and morphology characterization showed that both the KCS and SPS share similar microbial communities. Most of studied microorganisms are Gram-positive bacillus, cocci, streptobacillus and diplobacillus bacteria. These results provide some useful information in biodegradation of coal-contaminated soil for the further studies.
Библиографиялық сілтемелер
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27 Hooper D.U., Bignell D.E., Brown V.K., Brussard L.,Dangerfield J.M., Wall D.H.,Wardle D.A.,Coleman D.C., Giller K.E.,Lavelle P.,Van der Putten W.H., De Rviter P.C.,Rusek J., Silver W.L., Tiedje J.M.,Wolters V.(2000) Interactions between above ground and below ground biodiversity in terrestrial ecosystems patterns, mechanisms and feedbacks. Bio. Sci.vol. 50,pp.1046-1061.
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29 Atlas R.M., Bartha R.(2005) Microbial ecology fundamentals and applications, Fourth edition, Pearson Education, Singapore.
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31 Silva D.K.A., Freitas N.O., Sousa R.G. and Silva F.S.B.( 2012) Soil microbial biomass and activity under natural and regenerated forests and conventional sugarcane plantations in Brazil. Geoderma, vol. 189 , pp. 257-261.
32 Rodrigues R.C., Araujo R.C., Costa C.S., Lima A.J.T., OliveIira M.E., Cutrim Junior J.A.A., Santos F.N.S., Araujo J.S., Santos V.M. and Aruujo A.S.F .(2015) Soil microbial biomass in an agroforestry system of Northeast Brazil. Tropical Grasslands,vol. 3,pp. 41-48.
33 Powlson D.S., Brookes P.C., Christensen B.T.(1987) Measurement of microbial biomass provides an early indication of changes in total soil organic matter due to straw incorporation, Soil Biol. Biochem. vol.19 pp.159–164.
34 Jenkinson D.S., Ladd J.N.(1981) Microbial biomass in soils: measurement and turnover, in: E.A. Paul, J.N. Ladd (Eds.), Soil Bioc, Mercel Dekker, NewYork, pp. 415–471.
35 Singh R.S., Srivastava S.C., Ragubanshi A.S., Singh J.S., Singh S.P.C.(1991) Microbial, N and P in dry tropical savanna: Effects of burning and grazing, J. Appl. Ecol. vol.28 , pp.869–878.
36 Vitousek P.A.,Matson P.A.(1984) Mechanism of nitrogen retention in forest ecosystem: a field experiment, Sci. vol. 225 ,pp.51–52.
37 Mummey D.L., Smith J.L., Bolton H. Jr.(1997) Small-scale spatial and temporal variability of N2O flux from a shrub-steppe ecosystem. Soil Biol. Biochem. vol. 29, pp.1699–1706.
2 Mummey D. L., Stahl P. D.,Buyer J. S.(2002) Microbial biomarkers as an indicator of ecosystem recovery following surface mine reclamation. Appl.Soil .Ecol.vol.21,no.3,pp.251-259.
3 Fierer N., Breitbart M., Nulton J., Salamon P., Lozupone C., Jones R., Robeson M., Edwards R.A., Felts B., Rayhawk S., Knight R., Rohwer F., Jackson R.B.(2007) Metagenomic and small-subunit rRNA analyses reveal the genetic diversity of bacteria, archaea, fungi, and viruses in soil. Appl. Environ. Micr .vol.73,pp.7059–7066.
4 Liebich J.,Schadt C.W.,Chong S.C.,He Z.L.,Rhee S.K.,Zhou J.(2006) Improvement of oligonucleotide probe design criteria for functional gene microarrays in environmental applications. Appl. Environ. Micr .vol.72,pp.1688–1691.
5 Death RG.,Barquín J.(2012) Geographic location alters the diversity-disturbance response.Freshwater Sci. Vol.31.pp.636-646.
6 Schwartz M.,Brigham C.,Hoeksema J.,Lyons K.,et al.(2000) Linking biodiversity to ecosystem function: implications for conservation ecology. Oecologia .vol.122. pp.297-305.
7 van der Heijden MG.,Klironomos JN.,Ursic M.,Moutoglis P.,et al.(1998) Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nat. Vol.396,pp.69-72.
8 Sugden AM .(2000) Diversity & Ecosystem Resilience. Sci .vol.290 , pp.233-235.
9 Johnsen K.,Jacobsen CS.,Torsvik V., Sørensen J.(2001) Pesticide effects on bacterial diversity in agricultural soils-a review. Biol. Fert. Soils .vol. 33,pp.443-453.
10 Holden S.R.,Treseder K.K.(2013) A meta-analysis of soil microbial biomass responses to forest disturbances. Frontiers in Microbiology. vol.4 .pp.163.
11 Doran J.,Coleman D., Bezdicek D.,Stewart B.,Turco R.F.,Kennedy A.C., Jawson M.D.(1994)Microbial Indicators of Soil Quality.SSSA Special Publication Defining Soil Quality for a Sustainable Environment.
12 Harris J.A.,Bentham H., Birch P . (2003) Measurements of the soil microbial community for estimating the success of restoration. European Journal of Soil Science.vol.54.no.4,pp.801-808.
13 Poncelet D.M.,Cavender N.,Cutright T.J., Senko J.M. (2013) An assessment of microbial communities associated with surface mining-disturbed overburden. Environ Moni and Asses.vol.186.no.3,pp.1917-1929.
14 Gram H.C.(1884)"Über die isolierte Färbung der Schizomyceten in Schnitt- und Trockenpräparaten". Fortschritte der Medizin (in German). vol.2,pp.185–189.
15 Dasari TP, Hwang HM.(2010) The effect of humic acids on the cytotoxicity of silver nanoparticles to a natural aquatic bacterial assemblage. Sci Total Environ . vol.408 , pp.5817–5823.
16 Leboffe, M. J. and Pierce, B. E.(2010) Microbiology Laboratory Theory and Application, Third Edition. Morton Publishing Company.
17 Entry J.A.,Mills D.,Mathee K.,Jayachandran K.,Sojka R.E.(2008) Narasimhan G.Influence of irrigated agriculture on soil microbial diversity. Appli. Soil Eco. vol.40,pp.146-154.
18 Conrad R.(1996) Soil microorganisms as controllers of atmospheric trace grasses.Micr Rev.vol.60.pp.609-640.
19Whitman W.,Coleman D.,Weibe W.(1998) Prokaryotes: The unseen majority.Proc.Nat.Acad. Sci. USA. Vol.95,pp. 6578-6583.
20 Copley J. (2000) Ecology goes underground.Nat.vol. 406, pp.452-454.
21 Kozdroj J.,Van Elsas J.D.(2001)Structural diversity of microorganisms in chemically protrubed soil assed by molecular and cytochemical approaches.J.Micorbiol.Method.vol. 43,pp.197-212.
22 Johnsen K.,Jacobsen C.S.,Torsvik,V., Sorensen J.(2001) Pesticide effects on bacterial diversity in agricultural soils, A review. Biol. Fertil. Soil.vol. 33, pp.443-453.
23 Lee K.E.(1991) The diversity of soil organisms. In the biodiversity of microorganisms and invertebrates: its role in sustainable agriculture (Ed.D.L.Hawskworth),CBA International, Wallingford.
24 Torsvik V ., Ovreas L. Microbial diversity and function in soil: from genes to ecosystems. Curr.Opin Microbiol. 5 (2002):240-245.
25 Mishra R.R.Influence of soil environments and surface vegetation on soil microflora. Proc.Nat.Acad.Sci.(India) 26(1996):117-123.
26 Angers D. A.,Caron J.Plant-Induced changes in soil structure:Processes and feedbacks. Biogeochemistry.42(1998):55-72.
27 Hooper D.U., Bignell D.E., Brown V.K., Brussard L.,Dangerfield J.M., Wall D.H.,Wardle D.A.,Coleman D.C., Giller K.E.,Lavelle P.,Van der Putten W.H., De Rviter P.C.,Rusek J., Silver W.L., Tiedje J.M.,Wolters V.(2000) Interactions between above ground and below ground biodiversity in terrestrial ecosystems patterns, mechanisms and feedbacks. Bio. Sci.vol. 50,pp.1046-1061.
28 Fierer N.,Jackson R.B. (2006 ) The diversity and biogeography of soil bacterial communities. Proc. Natl. Acad. Sci.pp.103626-631.
29 Atlas R.M., Bartha R.(2005) Microbial ecology fundamentals and applications, Fourth edition, Pearson Education, Singapore.
30 Rinklebe J.,Langer U.(2006 ) Microbial diversity in three floodplain soils of the Elbe River (Germany).Soil Biol.&Biochem. pp.382144-2151.
31 Silva D.K.A., Freitas N.O., Sousa R.G. and Silva F.S.B.( 2012) Soil microbial biomass and activity under natural and regenerated forests and conventional sugarcane plantations in Brazil. Geoderma, vol. 189 , pp. 257-261.
32 Rodrigues R.C., Araujo R.C., Costa C.S., Lima A.J.T., OliveIira M.E., Cutrim Junior J.A.A., Santos F.N.S., Araujo J.S., Santos V.M. and Aruujo A.S.F .(2015) Soil microbial biomass in an agroforestry system of Northeast Brazil. Tropical Grasslands,vol. 3,pp. 41-48.
33 Powlson D.S., Brookes P.C., Christensen B.T.(1987) Measurement of microbial biomass provides an early indication of changes in total soil organic matter due to straw incorporation, Soil Biol. Biochem. vol.19 pp.159–164.
34 Jenkinson D.S., Ladd J.N.(1981) Microbial biomass in soils: measurement and turnover, in: E.A. Paul, J.N. Ladd (Eds.), Soil Bioc, Mercel Dekker, NewYork, pp. 415–471.
35 Singh R.S., Srivastava S.C., Ragubanshi A.S., Singh J.S., Singh S.P.C.(1991) Microbial, N and P in dry tropical savanna: Effects of burning and grazing, J. Appl. Ecol. vol.28 , pp.869–878.
36 Vitousek P.A.,Matson P.A.(1984) Mechanism of nitrogen retention in forest ecosystem: a field experiment, Sci. vol. 225 ,pp.51–52.
37 Mummey D.L., Smith J.L., Bolton H. Jr.(1997) Small-scale spatial and temporal variability of N2O flux from a shrub-steppe ecosystem. Soil Biol. Biochem. vol. 29, pp.1699–1706.
Жүктелулер
Жарияланды
2017-12-12
Как цитировать
Qiao, X., Tastambek, K., Akimbekov, N., Kayirmanova, G., Aidarhanova, G., & Zhubanova, A. (2017). Microbial analysis of coal polluted soils in the region of Karagandy, Kazakhstan. ҚазҰУ Хабаршысы. Биология сериясы, 72(3), 94–103. https://doi.org/10.1901/kbs1282
Шығарылым
Бөлім
МИКРОБИОЛОГИЯ
