ГЕНЕТИЧЕСКИЙ АНАЛИЗ АЛЛОПЛАЗМАТИЧЕСКИХ ЛИНИЙ ПШЕНИЦЫ С ИСПОЛЬЗОВАНИЕМ МАРКЕРОВ, ОПРЕДЕЛЯЮЩИХ СРОКИ КОЛОШЕНИЯ
DOI:
https://doi.org/10.26577/eb.2023.v95.i2.02Ключевые слова:
пшеница, аллоплазматические линии, засухоустойчивостьАннотация
Проведено изучение генетического разнообразия аллоплазматических линий пшениц, различающихся у стойчивости к засухе и по срокам вегетации с использованием маркеров к генам, определяющих сроки колошения. Объектами исследований служили девять аллолиний пшеницы, полученных от скрещивания T. dicoccum var Araratum (Host.) и T. aestivum сорта Мироновская 808, в результате объединения цитоплазмы одного вида с ядерным геномом другого вида и последующим многолетним отбором. С использованием ПЦР проводили анализ генов Vrn и Ppd, определяющих сроки колошения. Установлено, что большинство аллолиний по рецессивным генам яровизации и фотопериода наследуют генотип сорта Мироновская-808. Исключением является линия D-n-05, у которой выявлен доминантный аллель Vrn-B1c. Анализ генов Ppd-D1 с использованием аллель-специфичных праймеров показал наличие доминантного аллеля Ppd-D1a в гетерозиготном состоянии у D-d-05b и D-d-05.
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