Obtaining transgenic tobacco plants expressed transcription factor AtDREB2A gene.
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Keywords:
transcription factor, AtDREB2A, drought, salinity, tobacco,Abstract
The task of this work is obtaining of transgenic tobacco plants, expressed transcription factor AtDREB2A gene in native and mutant forms for investigation of molecular mechanisms of plant resistance to drought. DNA-fragment coding transcription factor AtDREB2A gene from Arabidopsis thaliana was amplified and deletion mutagenesis of coding sequence was carried out with the view of obtaining constitutively active protein. Both of sequences were cloned under control of 35S-promoter CaMV or inducible rd29A promoter and also different variants of translation enhancers. The transgenic plants consisting coding sequences of transcription factor AtDREB2A in native and mutant forms in its genome were gained.References
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2 Rodríguez M. et al. Molecular aspects of abiotic stress in plants. //Biotecnología Aplicada. – 2005. – Vol. 22. - № 1.
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3 Seki M. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high salinity stresses using a full-length cDNA microarray // The Plant Journal. – 2002. - №31. – P. 279–292.
4 Bohnert H. J. et al. A genomics approach towards salt stress tolerance // Plant Physiol. Biochem. – 2001. - №39. – P.295-311.
5 Chen W. Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses // Plant Cell. – 2002. - №14. - № – P. 559-74.
6 Fowler S. Arabidopsis transcriptome pro-filing indicates that multiple regulatory pathways are activated during cold
acclimation in addition to the CBF cold response pathway // Plant Cell. – 2002. - №14. – P. 1675-90.
7 Abdelaty S. Plant AP2/ERF transcription factors // Genetika. – 2003. - Vol. 35. - №1. – P. 37-50.
8 Qiang L. Two Transcription Factors, DREB1 and DREB2, with an EREBP/AP2 DNA Binding Domain Separate
Two Cellular Signal Transduction Pathways in Drought- and Low-Temperature-Responsive Gene Expression, Respectively, in Arabidopsis // The Plant Cell. – 1998. – Vol. 10. – P. 1391–1406.
9 Hsieh T.H. Heterology expression of the Arabidopsis C-repeat/ dehydration response element binding factor 1 gene
confers elevated tolerance to chilling and oxidative stresses in transgenic tomato // Plant Physiol. – 2002. - №129. – P.
1086-94.
10 Agarwal P.K.et al. Role of DREB transcription factors in abiotic and biotic stress tolerance in plants // Plant Cell. –
2006. - №25. – P. 1263–1274.
11 Shi-Qing Gao et al. A cotton (Gossypium hirsutum) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat // Plant Cell Rep. – 2009. - №28. - P.301–311.
12 Sakuma Y. et al. Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in droughtresponsive
gene expression // Plant Cell. – 2006. - №18. – P. 1292–1309.
13 Qin F. Arabidopsis DREB2A-interacting proteins function as RING E3 ligases and negatively regulate plant drought
stress-responsive gene expression // Plant Cell. – 2008. – Vol. 20. – P. 1693-1707.
14 Agarwal P. et al. Stress-inducible DREB2A transcription factor from Pennisetum glaucum is a phosphoprotein and
its phosphorylation negatively regulates its DNA-binding activity // Mol. Genet. Genomics. – 2007. – Vol. 277 – P.
189–198.
15 Писаренко, Наргилова и др. Получение растений картофеля, экспрессирующих транскрипционный фактор
AtDREB2A //Вестник КазНУ. Серия биологическая. – 2013. - №3/1 (59). – С. 164-168
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Pisarenko, A., Nargilova, R. M., Karpova, O. V., & Iskakov, B. K. (2015). Obtaining transgenic tobacco plants expressed transcription factor AtDREB2A gene. Experimental Biology, 60(2), 320–323. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/962
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