Investigation of ability of the grapevine virus A to infect Nicotiana Benthamiana by using Agrobacterium infiltration
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Keywords:
Grapevine virus A genome, a viral vector, a binary vector, agroinfiltration, heterologous gene,Abstract
In this work was created binary vector carrying the complete genome of an infectious clone of the virus A grape with flanking regulatory sequences (35S- promoter , 35S- terminator ). Agrobacterium was transformed by this vector for delivery viral genome into plants by using agroinfiltration . Agroinfiltration was carried by bacteria of different optical density, in order to identify the optimum density for tobacco. Early signs of infection were noticed at 7-10 days after agroinfiltration in plants, which confirms the continuation of infectivity of the virus.References
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2 Yuri Gleba, Sylvestre Marillonnet,Victor Klimyuk. Engineering viral expression vectors for plants: the ‘full virus’ and the ‘deconstructed virus’ strategies // Current Opinion in Plant Biology.- 2004.- Vol.7.-P. 182-188.
3 Sabrina Haviv , Nurbol Galiakparov , Dariusz E. Goszczynskib,Ozgur Batumana, Henryk Czosnek, Munir Mawassi. Engineering the genome of Grapevine virus A into a vector for expression of proteins in herbaceous plants // Journal of Virological Methods.-2006,- Vol. 132/- P. 227-231.
4 H.Inoue, H.Nojima, H.Okayama. High efficiency transformation of Escherichia coli with plasmids // Gene.-1990.- 96.- P.23-8.
5 Imogen A Sparkes, John Runions, Anne Kearns. Chris Hawes. Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stablytransformed plants // Nature protocols.-2006.-Vol.1, № 4.- P. 2021- 2025.
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Kopytina, D. A., & Galiakparov, N. N. (2015). Investigation of ability of the grapevine virus A to infect Nicotiana Benthamiana by using Agrobacterium infiltration. Experimental Biology, 60(1), 195–199. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/93
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Bioengineering of Plants
