Genotyping of perspective in Kazakhstan grape varieties by SSR microsatellite markers
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Keywords:
grape, genotyping, ssr markers, DNA, PCR,Abstract
In this paper, applied to grapes cost-effective method of genotyping 6 microsatellite markers using the corresponding pairs of unlabeled specific primers and three universal oligonucleotides labeled with different fluorescent dyes. This technique is used for genotyping of several promising Kazakhstan varieties and their parental forms . Analysis conducted by researchers grapes used set of 6 markers and mikrosattelite: VVS2, VVMD5, VVMD7, VVMD27, ssrVrZAG62 and ssrVrZAG79 confirmed for Kazakhstan’s varieties parent-offspring relationship." The method will be used for genetic certification of grapevine varieties in Kazakhstan.References
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2 Miedaner T., Korzun V. Marker-assisted selection for disease resistance in wheat and barley breeding. Phytopathology. – 2012. –V. 102. – P. 560-566.
3 Paux E., Sourdille P., Mackay I., Feuillet C. Sequence-based marker development in wheat: advances and applications to breeding //Biotechnol Adv. – 2012. –V. 30. –P. 1071-1088.
4 Powel W., Machray G.C., Provan J. Polymorphism revealed by simple sequence repeats // Trends Plant Sci.–1996. –V. 1.–P. 215-222.
5 Doyle J.J. and Doyle JL. Isolation of plant DNA from fresh tissue // Focus. –1990.–Vol.12.–P.13-15
6 AubakirovaK., OmashevaM., RyabushkinaN., Tazhibaev T., Kampitova G., GaliakparovN. Evaluation of five protocols for DNA extraction from leaves of Malussieversii, Vitis vinifera, and Armeniaca vulgaris // GMR. –accepted.
7 This P., Jung A., Boccacci P., Borrego J., Botta R., Costantini L., Crespan M.,. Dangl . C. Eisenheld F. Ferreira-Monteiro . S. Grando . J. Iba.n˜ ez T. Lacombe . V. Laucou . R. Magalha˜ es G.S., Meredith C.P., Milani N., Peterlunger E., Regner F., Zulini L., Maul E. Development of a standard set of microsatellite reference alleles for identification of grape cultivars // Theor Appl Genet.– 2004. –Vol.109. –P.1448-1458 doi: 10.1007/s00122-004-1760-3 PMID: 15565426
8 Аубакирова К.П., Омашева М.Е., Рябушкина Н.А., Береснева Л.В.,ГалиакпаровН.Н. Использование универсальных флуоресцентно-меченых праймеров в генотипировании казахстанских сортов винограда по микросателлитным маркерам// Биотехнология теория и практика.-2013. – №2.- С.35-41.
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Aubakirova, K. P., Omasheva M. Е., Ryabushkina N. А., Tazhibaev Т. S., Suyunbaeva G. М., & Galiakparov, N. N. (2015). Genotyping of perspective in Kazakhstan grape varieties by SSR microsatellite markers. Experimental Biology, 60(1), 186–190. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/91
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Bioengineering of Plants
