ТРАНСГЕННЫЕ РАСТЕНИЯ ДЛЯ ФИТОРЕМЕДИАЦИИ
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Keywords:
transgenic plants, glutathione,Abstract
It was reviewed the literature in recent years about the potential use of transgenic plants to phytoremmediation soils contaminated by heavy metals. The possibility of obtaining a number of plant transformants with increased ability to accumulate in intracellular structures (mainly in the vacuoles) and in the intercellular space of conjugates of endogenous compounds with toxicants was shown. Genetic engineering research works to improve the metal accumulation ability of plants are conducting intensively, especially in recent years. Manipulation of the expression of enzymes like as γ-glytamyl -Cys-synthetase involved in the synthesis of glutathione and phytochelatins can be an excellent approach for improving plant resistance, as phytochelatins synthase enzyme may not be a limiting factor for the synthesis of phytochelatins by constitutive expression in plants. Regulation of glutathione synthesis promotes the accumulation of heavy metals and increase the tolerance of the transgenic plants. The possibility of using gene of the enzyme glutathione-S-transferase to create transgenic plants it was shown.References
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3. Foyer C.H., Haliwell B. The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism // Planta. - 1976. – Vol. 133. – P. 21-25.
4. Marrs K. The functions and regulation of glutathione S-transferases in plants // Ann. Rev. Plant Physiol. Plant Mol. Biol. –1996. – Vol. 47. – P.127-158.
5. Zenk M.H. Heavy metal detoxification in higher plants: a review // Gene. – 1996. – Vol. 179. – P. 21-30.
6. Chen J., Zhou J., Goldsbrough P.B. Characterization of phytochelatin synthase from tomato // Physiol Plant. – 1997. –Vol. 101. – P. 165-172.
7. Steffens J.C. The heavy metal-binding peptides of plants // Ann. Rev. Plant Physiol. Plant Mol. Biol. – 1990. – V. 41. –Pp. 553-575.
8. Foyer C.H., Souriau N., Perret S., Lelandais M., Kunert K.J., Pruvost C., Jouanin L. Over-expression of glutathione reductase but not glutathione synthetase leads to increases in antioxidant capacity and resistance to photoinhibition in poplar trees // Plant Physiol. – 1995. – Vol. 109. – P. 1047-1057.
9. Schneider S., Bergmann L. Regulation of glutathione synthesis in suspension cultures of parsley and tobacco // Bot Acta. – 1995. – Vol. 108. – P. 34-40.
10. Rauser W.E., Schupp R., Rennenberg H. Cysteine, γ-glutamylcysteine and glutathione levels in maize seedlings. Distribution and translocation in normal and cadmium-exposed plants // Plant Physiol. – 1991. – Vol. 97. – P. 128-138.
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13. Marchiol L., Leita L., Martin M., Peressotti A., Zerbi G. Physiological responses of two soybean cultivars to cadmium // J.Environ. Qual. – 1996. – Vol. 25. – P. 562-566.
14. Petit C.M., van de Geijn S.C. In vivo measurements of cadmium (115 mM Cd) transport and accumulation in steams of intact tomato plants (Lycopersicon esculentum Mill). I. Long distance transport and local accumulation // Planta. – 1978. – Vol. 138. –P. 137-143.
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Атабаева, С. Д., & Кенжебаева, С. С. (2017). ТРАНСГЕННЫЕ РАСТЕНИЯ ДЛЯ ФИТОРЕМЕДИАЦИИ. Experimental Biology, 56(4), 280–285. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/730
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Physical and chemical biology
