The activity of detoxifying enzymes in larvae of the locusts locusta migratoria l. the development of fungal infections of metarhizium anisopliae
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Keywords:
Locusta migratoria L., Metarhizium anisopliae, nonspecific esterase, glutathione-S-transferase, activity,Abstract
The analysis of the activity of nonspecific esterases and glutathione-S-transferase in homogenates of the whole body, lymph and fat body in larvae of the locusts Locusta migratoria L. the development of fungal infections of Metarhizium anisopliae (Metsch.) Sorokin. It is established that exposure to a lethal dose of the fungus (LC80), followed by stimulation of detoxifying enzymes in the homogenate of the whole body of larvae of L. migratoria on the third day after infection. It is shown that there is an increase of activity of nonspecific esterases and glutathione-S-transferase in the lymph and fat body of infected insects on day 3 of infection. By the sixth day of mycosis in the “hot” during the infection process in insects a decrease in enzyme activity to control values. Activation of components of the detoxification system in the initial phase of acute fungal infections may indicate involvement of detoxifying enzymes in the defense reactions of insects against fungal infection.References
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26 Habig W.H., Pabst M.J., Jakoby W.B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. // J.Biol. Chem. – 1974. – V.249. – P. 7130-7139.
27 Gillespie J.P., Burnett C., Charnley A.K. The immune response of the desert locust Schistocerca gregaria during mycosis of the entomopathogenic fungus Metarhizium anisopliae var acridum // J. Insect Physiol. – 2000. – V.46. – Р. 429-437.
2 Leger R.J.St., Cooper R.M., Charnley A.K. The effect of melanization of Manduca sexta cuticle on growth and infection by Metarhizium anisopliae // J. Invertebr. Pathol. – 1988. – V. 52. – P. 459-470.
3 Серебров В.В., Алексеев А.А., Глупов В.В. Изменение активности и спектра эстераз гемолимфы гусениц вощиной моли galleria mellonella L. (Lepidoptera; Pyralidae) при микрозах // Известия РАН. Сер. биол. – 2001. – № 5. – С. 588-592.
4 Серебров В.В., Киселев А.А., Глупов В.В. Изучение некоторых факторов синергизма между энтомопатогенными грибами и химическими инсектицидами // Микология и фитопатология. – 2003. – Т.1. – Вып. 37. – С. 76-82.
5 Серебров В.В., Гербер О.Н., Малярчук А.А., Мартемьянов В.В., Алексеев А.А., Глупов В.В. Влияние энтомопатогенных грибов на активность детоксицирующих ферментов гусениц пчелиной огневки Galleria mellonella L. (Lepidoptera, Pyralidae) и роль детоксицирующих ферментов при формировании резистентности насекомых к энтомопатогенным грибам. // Известия РАН. Сер. биол. – 2006. – №6. – С. 581-586.
6 Vilcinskas A., Jegorov A., Landa Z. et al. Effect of beauverolide L and cyclosporin A on humoral and cellular immune response of the greater wax moth, Galleria mellonella // Comp. Biochem. Physiol. – 1999. – V.122. – P. 83-92.
7 Hajek A.E., Leger R.J.St. Interactions Between Fungal Pathogens and Insect Hosts // Annu. Rev. Entomol. – 1994. – V.39. – P. 293-322.
8 James P.J., Charnley A.K., Reynold S.E. The effect destruxins on the structure and function of insect malpighia tubus // IOBC WPRS Bulletin. – 1994. – V. 17. – P. 218-221.
9 Charnley A.K. Fungal pathogens of insects: cuticle degrading enzymes and toxins // Advances in Botanical Research. – 2003. – Р. 241-321.
10 Li X., Schuler M.A., Berenbaum M.R. Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics. // Annu. Rev. Entomol. – 2007. – V. 52 – P. 231–253.
11 Рославцева С.А., Баканова Е.И., Еремина О.Ю. Эстеразы членистоногих и их роль в механизмах детоксикации инсектоакарицидов // Известия РАН. Сер. биол. – 1993. –№ 3. – С. 368-375.
12 Terriere L.C. Induction of detoxication enzymes in insects // Ann. Rev. Entomol. – 1984. – V. 29. – P. 71-88.
13 Papadopoulos A.I., Boukouvala E., Kakaliouras G., Kostaropoulos J., Papadopoulou-Mourkidou E. Effect of organophosphate and pyrethroid insecticides on the expression of GSTs from Tenebrio molitor pupae // Pesticide Biochem. Physiol. – 2000. – V. 68. – P.26-33.
14 Колесниченко Л.С., Кулинский В.И. Глутатионтрансферазы // Усп. совр. биол. – 1989. – Т.107. – Вып.2. – С. 179-194.
15 Баканова Е.И., Еремина О.Ю., Рославцева С.А. Свойства и функции глутатион-З-трансферазы членистоногих // Известия РАН. Сер. биол. – 1992. –№ 4. – С. 537-545.
16 Small G.J., Hemingway J. Molecular characterization of the amplified carboxylesterase gene associated with organophosphorus insecticide resistance in the brown planthopper, Nilaparvata lugens // Insect Mol. Biol. – 2000. – V. 9. – P. 647-653.
17 Pasteur N., Nance E., Bons N. Tissue localization of overproduced esterases in the mosquito Culex pipiens (Diptera: Culicidae) // J. Med. Entomol. – 2001. – V.38. – P. 791-801.
18 Field LM, Devonshire AL. Evidence that the E4 and FE4 esterase genes responsible for insecticide resistance in the aphid Myzus persicae (Sulzer) are part of a gene family // Biochem. J. – 1998. – V.330. – P. 169–173.
19 Hemingway J, Hawkes N, Prapanthadara L, Jayawardenal KGI, Ranson H. The role of gene splicing, gene amplification and regulation in mosquito insecticide resistance. // Philos. Trans. R. Soc. London B. – 1998. – V. 353. – № 1376. – P.1695–1699.
20 Field LM. Methylation and expression of amplified esterase genes in the aphid Myzus persicae (Sulzer) // Biochem. J. – 2000. – V. 349. – P.863–868.
21 Hawkes N.J., Hemingway J. Analysis of the promoters for the -esterase genes associated with insecticide resistance in the mosquito Culex quinquefasciatus // Biochim. Biophys. Acta. – 2002. – V. 1574. – P.51-62.
22 Воронцова Я.Л., Ершов Н.И., Глупов В.В. Влияние микроспоридии Vairimorpha ephestiae (Microsporidia: Burenellidae) на активность и спектр неспецифических эстераз различных тканей личинок большой пчелиной огневки Galleria mellonella (Lepidoptera: Pyralidae) // Паразитология. – 2006. – Т. 40. – № 1. – С. 74-84.
23 Shiotsuki T., Kato Y. Induction of carboxylesterase isozymes in Bombyx mori by E. coli infection //Biochemistry and Molecular Biology. – 1999. – V. 29. – Р. 731-736.
24 Xia Y., Dean P., Judge A.J., Gillespie J.P., Clarkson J.M., Charnley A.K. Acid phosphatases in the haemolymph of the desert locust Schistocerca gregaria, infected with the entomopathogenic fungus Metarhizium anisopliae // J. Insect Physiol. – 2000. – V. 46. – Р. 1249-1257.
25 Asperen K. Van. A study of housefly esterase by means of a sensitive colorimetric method // J. Insect Physiol. – 1962. – V. 8. – P. 401-416.
26 Habig W.H., Pabst M.J., Jakoby W.B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. // J.Biol. Chem. – 1974. – V.249. – P. 7130-7139.
27 Gillespie J.P., Burnett C., Charnley A.K. The immune response of the desert locust Schistocerca gregaria during mycosis of the entomopathogenic fungus Metarhizium anisopliae var acridum // J. Insect Physiol. – 2000. – V.46. – Р. 429-437.
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Slyamova, N. D., Dubovskiy, I. M., Belgibaeva, A. B., & Duisembekov, B. A. (2015). The activity of detoxifying enzymes in larvae of the locusts locusta migratoria l. the development of fungal infections of metarhizium anisopliae. Experimental Biology, 55(3), 99–105. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/286
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