Agrobacterium-mediated transformation of rice the salt tolerance gene TaNHX1
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Keywords:
rice, agrotransformation, salt tolerance, gen, regenerant,Abstract
The rice variety Bakanas was co-transformed with the salt tolerance gene TaNHX1 and the HPT selective marker gene using Agrobacterium-mediated transformation. Salt-tolerant transgenic plants were obtained. The resulting salt-tolerant transgenic plants were tested by PCR analysis using Actin 1 promoter forward primer and NosTr reverse primer.References
1 Жимулев И.Ф. Общая и молекулярная генетика. – Новосибирск: Сибирское университетское издательство. – 2003. –С. 157-148.
2 Christie P.J., Atmakuri K., Krishnamoorthy V., Jakubowski S., Cascales E. Biogenesis, architecture and function of bacterial type IV secretion systems //Annu. Rev. Microbiol. – 2005. – Vol.59. – P.451–485.
3 Juhas M., Crook D.W. Hood D.W. Type IV secretion systems: tools of bacterial horizontal gene transfer and virulence // Cellular Microbiology. – 2008. – Vol.10. – P.2377–2386.
4 White C.E., Winans S.C. Сell communication in the plant pathogen Agrobacterium tumefaciens // Philos. Trans. R. Soc. Lond. B. Biol. Sci. – 2007. – Vol.362. – P.1135–1148.
5 Рябушкина Н.А., Галиакпаров Н.Н., Рахимбаев И.Р. Трансформация растений с помощью агробактерий. Вероятные последствия // Биотехнология. – 2009. – N 4. – С.29-37.
6 http://www.ru.wikipedia.org
2 Christie P.J., Atmakuri K., Krishnamoorthy V., Jakubowski S., Cascales E. Biogenesis, architecture and function of bacterial type IV secretion systems //Annu. Rev. Microbiol. – 2005. – Vol.59. – P.451–485.
3 Juhas M., Crook D.W. Hood D.W. Type IV secretion systems: tools of bacterial horizontal gene transfer and virulence // Cellular Microbiology. – 2008. – Vol.10. – P.2377–2386.
4 White C.E., Winans S.C. Сell communication in the plant pathogen Agrobacterium tumefaciens // Philos. Trans. R. Soc. Lond. B. Biol. Sci. – 2007. – Vol.362. – P.1135–1148.
5 Рябушкина Н.А., Галиакпаров Н.Н., Рахимбаев И.Р. Трансформация растений с помощью агробактерий. Вероятные последствия // Биотехнология. – 2009. – N 4. – С.29-37.
6 http://www.ru.wikipedia.org
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Iskakova, A. B., Uteulin, K. R., Bary, G., & Ismagul, A. Z. (2015). Agrobacterium-mediated transformation of rice the salt tolerance gene TaNHX1. Experimental Biology, 55(3), 63–67. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/280
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BIOTECHNOLOGY, BIOCHEMISTRY AND PLANTS PHYSIOLOGY
