ПРИМЕНЕНИЕ МИКРООРГАНИЗМОВ-АНТАГОНИСТОВ ДЛЯ ЗАЩИТЫ ЯЧМЕНЯ ОТ ФИТОПАТОГЕНОВ

L.V.  Ignatova; E.V.  Brazhnikova; М.А.  Chervyakov; E.V.  Moskvina; A.P.  Chernykh; S.R.  Muradova; Р.К.  Sydykbekova

doi:10.26577/bb106120267

Authors

L.V. Ignatova Al-Farabi Kazakh National University, Almaty, Kazakhstan https://orcid.org/0000-0002-0811-6775
E.V. Brazhnikova Al-Farabi Kazakh National University, Almaty, Kazakhstan https://orcid.org/0000-0003-3807-6847
М.А. Chervyakov Al-Farabi Kazakh National University, Almaty, Kazakhstan https://orcid.org/0009-0007-9565-9164
E.V. Moskvina Al-Farabi Kazakh National University, Almaty, Kazakhstan https://orcid.org/0009-0009-2945-1220
A.P. Chernykh Al-Farabi Kazakh National University, Almaty, Kazakhstan https://orcid.org/0009-0002-7142-2684
S.R. Muradova Al-Farabi Kazakh National University, Almaty, Kazakhstan https://orcid.org/0009-0005-3058-4806
Р.К. Sydykbekova Al-Farabi Kazakh National University, Almaty, Kazakhstan https://orcid.org/0000-0002-7924-4977

DOI:

https://doi.org/10.26577/bb106120267

Keywords:

biopreparation, immobilization of microorganisms, bacterial association, potassium humate, phytopathogens.

Abstract

In order to enhance plant resistance to biotic and abiotic stresses, an association of agronomically valuable microorganisms based on rhizospheric and endophytic bacteria was developed. The use of microbial associations is considered a more effective approach compared with monocultures, since microbial consortia possess a broader spectrum of biological activity and are capable of synergistic interactions within agroecosystems.

For the formation of the association, the strains Pseudomonas flavescens D5, Serratia proteamaculans B5, and Pseudomonas fluorescens D7 were selected, characterized by plant growth-promoting and biocontrol properties. A comprehensive evaluation of the agronomically valuable properties of the association was carried out, including biosafety/biocompatibility, halotolerance, synthesis of indole-3-acetic acid, phosphate-mobilizing activity, and antifungal activity. It was found that the LYA23 association demonstrated increased IAA production compared with individual strains, reaching 63.4 μg/mL, pronounced phosphate-mobilizing activity (293 μg/mL), and maintained growth at NaCl concentrations up to 5%. The association exhibited high antagonistic activity against Fusarium graminearum and Fusarium oxysporum, forming growth inhibition zones up to 3.5 ± 0.1 cm. The polymer matrix composed of the biopolymers pullulan, pectin, and polyhydroxyalkanoate in a ratio of 2:1:0.1 was recognized as optimal for cell immobilization. The addition of potassium humate further enhanced the plant growth-promoting effect of the preparation. In vegetation experiments, treatment of barley seeds of the Arna cultivar increased germination up to 98.6% and significantly increased root and shoot biomass compared with the control.

Author Biographies

L.V. Ignatova, Al-Farabi Kazakh National University, Almaty, Kazakhstan

Ignatova Lyudmila Viktorovna – Professor of the Department of Biotechnology, Faculty of Biology and Biotechnology, NJSC Al-Farabi Kazakh National University (Almaty, Kazakhstan, email: lyudmila.ignatova@kaznu.kz)

E.V. Brazhnikova, Al-Farabi Kazakh National University, Almaty, Kazakhstan

Brazhnikova Elena Valerievna – PhD, Lecturer of the Department Biotechnology, Faculty of Biology and Biotechnology, NJSC Al-Farabi Kazakh National University (Almaty, Kazakhstan, email: polb_4@mail.ru)

М.А. Chervyakov, Al-Farabi Kazakh National University, Almaty, Kazakhstan

Chervyakov Mikhail Alexandrovich (corresponding author) – Researcher, Department of Biotechnology, Faculty of Biology and Biotechnology, NJSC Al-Farabi Kazakh National University (Almaty, Kazakhstan, email: mihach999@gmail.com)

E.V. Moskvina, Al-Farabi Kazakh National University, Almaty, Kazakhstan

Moskvina Ekaterina Vyacheslavovna – Research Assistant, Department of Biotechnology, Faculty of Biology and Biotechnology, NJSC Al-Farabi Kazakh National University (Almaty, Kazakhstan, email: katysha0205@gmail.com)

A.P. Chernykh, Al-Farabi Kazakh National University, Almaty, Kazakhstan

Chernykh Angelina Petrovna – Research Assistant, Department of Biotechnology, Faculty of Biology and Biotechnology, NJSC Al-Farabi Kazakh National University (Almaty, Kazakhstan, email: Angelina_gizbreht@mail.ru)

S.R. Muradova, Al-Farabi Kazakh National University, Almaty, Kazakhstan

Muradova Susana Rustamkyzy – Researcher, Department of Biotechnology, Faculty of Biology and Biotechnology, NJSC Al-Farabi Kazakh National University (Almaty, Kazakhstan, email: muradova.susana@mail.ru)

Р.К. Sydykbekova, Al-Farabi Kazakh National University, Almaty, Kazakhstan

Sydykbekova Raikhan Konaevna – Associate Professor at the Department Biotechnology, Faculty of Biology and Biotechnology, NJSC Al-Farabi Kazakh National University (Almaty, Kazakhstan, email: konaevna@gmail.com)