Use of modern metegenomics approaches in evaluation of soil microbiota of West Kazakhstan area
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Keywords:
microbiome, soil microorganisms, PCR, sequencing, taxonomy, soil type, DNA, agroecology, monitoring, molecular biologyAbstract
This article describes a method to assess the soil microbiome with application of modern metagenomics. The method to clean soil DNA using adsorption on purified silicon. Identified data for optimization of PCR primer design. To assess the soil metagenome library composed of amplicons and held its initial analysis. Revealed the most prevalent phyla in these soil samples.References
1 Acinas, S.G. PCR-induced sequence artifacts and bias: insights from comparison of two 16S rRNA clone libraries
constructed from the same sample [Text] / R. Sarma-Rupavtarm, V. Klepac-Ceraj // Appl. Environ. Microbiol. – 2005. –V.12, № 71. – P. 8966 – 8969. – ISSN 1098-5336.
2 Bartram, A.K. Generation of Multimillion-Sequence 16S rRNA Gene Libraries from Complex Microbial Communities
by Assembling Paired-End Illumina Reads [Text] / M.D. Lynch, J.C. Stearns // Appl Environ Microbiol. – 2011.
– V.11, № 77. – P. 3846 – 3852. – ISSN 1098-5336.
3 Brajesh, K. Singh, Soil genomics [Text] / Colin D. Campbell, Soren J. Sorenson et al. // Nature Reviews Microbiology
– 2009. – V.7 – P. 756. – ISSN 1740-1526.
4 Gerlach, W. WebCARMA: a web application for the functional and taxonomic classification of unassembled
metagenomic reads [Text] / S. Jünemann, F. Tille // BMC Bioinformatics. – 2009. – V.10. – P.430. – ISSN 1471-2105.
5 Warnecke, F. Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite [Text]
/ P. Luginbühl, N. Ivanova // Nature. – 2007. – V.450. – P. 560 – 565. – ISSN 0028-0836.
constructed from the same sample [Text] / R. Sarma-Rupavtarm, V. Klepac-Ceraj // Appl. Environ. Microbiol. – 2005. –V.12, № 71. – P. 8966 – 8969. – ISSN 1098-5336.
2 Bartram, A.K. Generation of Multimillion-Sequence 16S rRNA Gene Libraries from Complex Microbial Communities
by Assembling Paired-End Illumina Reads [Text] / M.D. Lynch, J.C. Stearns // Appl Environ Microbiol. – 2011.
– V.11, № 77. – P. 3846 – 3852. – ISSN 1098-5336.
3 Brajesh, K. Singh, Soil genomics [Text] / Colin D. Campbell, Soren J. Sorenson et al. // Nature Reviews Microbiology
– 2009. – V.7 – P. 756. – ISSN 1740-1526.
4 Gerlach, W. WebCARMA: a web application for the functional and taxonomic classification of unassembled
metagenomic reads [Text] / S. Jünemann, F. Tille // BMC Bioinformatics. – 2009. – V.10. – P.430. – ISSN 1471-2105.
5 Warnecke, F. Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite [Text]
/ P. Luginbühl, N. Ivanova // Nature. – 2007. – V.450. – P. 560 – 565. – ISSN 0028-0836.
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Sergaliev, N. H., Andronov, E. E., Pinaev, A. G., Kakishev, M. G., & Zaharyan, R. A. (2015). Use of modern metegenomics approaches in evaluation of soil microbiota of West Kazakhstan area. Experimental Biology, 57(1), 133–138. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/223
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MICROBIOLOGY
