Selection of culture medium for phosphate solubilizing bacteria
Keywords:
phosphate mobilizing bacteria, activity, culture medium,Abstract
Culture medium for the newly isolated phosphatemobilizing bacteria – individual strains and associations – has been selected. Selection was carried out on the modifiedSzegimethodamong recommended for the cultivation of phosphatemobilizingmicroorganismsbasic and special culture media such as FPB, Muromtsevmediumand NBRIP (National Botanical Research Institute’s Phosphate growth medium). Pure liquid Muromtsevmediumwithout inoculum has been used as a control. Phosphatemobilizing bacterial activity was quantified by the diameter of the halo regions without deducting diameter wells and expressed in mm. Maximum biomass accumulation observed when ultivatedbacteria on FPB, the maximum activity for the mobilization of phosphates marked when cultivatedon NBRIP. NBRIPis recommendedfor the industrial production of biomass phosphate mobilizing bacteria.References
1 Lambers H., Shane M.W., Cramer M.D., Pearse S.J., Veneklaas E.J. Root structure andfunctioning for efficient acquisition of phosphorus: matching morphological and physiological traits // Ann. Bot. –2006. – Vol. 98. – P. 693-713.
2 Marschner H. Mineral nutrition of higher plants. London, Academic Press.- 1995. – 889 p.
3 Lambers H., Chapin F.S., Pons T.L. Plant Physiological Ecology. Second Edition. Springer. – 2008. –604 p.
4 Bieleski R.L. Phosphate pools, phosphate transport, and phosphate availability. // Ann. Rev.PlantPhysiol. –1973. – Vol.24 –P. 225–252.
5 DunajcevI.A. Vydelenie fosfatsoljubilizirujushhih mikroorganizmov i izuchenie vozmozhnosti ih ispol’zovanija v promyshlennosti i sel’skom hozjajstve: avtoref. … diss. kand. biol. nauk: 03.02.03;03.01.06. – Obolensk: Nauka, 2010. –29 p.
6 Thakuria D., Talukdar1 N.C., Goswami1 C., Hazarika1 S., Boro R.C., Khan M.R. Character-ization and screening of bacteria from rhizosphere of rice grown in acidic soils of Assam // Curr. Sci. –2004. – Vol. 86. – P.978-685.
7 Mehrvarz S., Chaichi M.R., AlikhaniH.A.Effects of Phosphate Solubilizing Microorganisms and Phosphorus Chemical Fertilizeron Yield and Yield Components of Barely (Hordeum vulgare L.) //American-Eurasian J. Agric. & Environ. Sci. – 2008. – Vol. 3(6). – P. 822-828.
8 Pérez-García A., Romero D., de Vicente A. Plant protection and growth stimulation by mi-croorganisms:biotechnological application of Bacillusin agriculture // Curr. Opin. Biotechnol. – 2011. – Vol. 22. – P. 1-7.
9 Yasmin H., Bano A. Isolation and characterization of phosphate solubilizing bacteria from rhizosphere soil of weeds of khewra salt range and attock // Pakistan Journal of Botany. – 2011. – №3. – P.1663-1668.
10 Khan A.A, Jilani G., Akhtar M.S., Naqvi S., Rasheed M. Phosphorus Solubilizing Bacteria: Occurrence, Mechanisms and their Role in Crop Production // J. Agric. Biol. Sci. 2009. –Vol.1(1). – P.
48-58.
11 Vahed H.S., Shahinrokhsar P., Heydarnezhad F. Performance of phosphate solubilizing bacteriafor improving growth and yield of rice (Oryza Sativa L.) in the presence of phosphorus fer-tilizer// Int. J. Agri. Crop Sci. – 2012. – Vol. 4(17). – P. 1228- 1232.
12 Nautiyal C.S. An efficient microbiological growth medium for screening phosphate solubil-izing microorganisms // FEMS Microbiol Lett. – 1999. – Vol. 170(1). – P. 265-270.
13 Sjegi J. Metody pochvennoj mikrobiologii. – 1983. – M.: Kolos. –162 p.
2 Marschner H. Mineral nutrition of higher plants. London, Academic Press.- 1995. – 889 p.
3 Lambers H., Chapin F.S., Pons T.L. Plant Physiological Ecology. Second Edition. Springer. – 2008. –604 p.
4 Bieleski R.L. Phosphate pools, phosphate transport, and phosphate availability. // Ann. Rev.PlantPhysiol. –1973. – Vol.24 –P. 225–252.
5 DunajcevI.A. Vydelenie fosfatsoljubilizirujushhih mikroorganizmov i izuchenie vozmozhnosti ih ispol’zovanija v promyshlennosti i sel’skom hozjajstve: avtoref. … diss. kand. biol. nauk: 03.02.03;03.01.06. – Obolensk: Nauka, 2010. –29 p.
6 Thakuria D., Talukdar1 N.C., Goswami1 C., Hazarika1 S., Boro R.C., Khan M.R. Character-ization and screening of bacteria from rhizosphere of rice grown in acidic soils of Assam // Curr. Sci. –2004. – Vol. 86. – P.978-685.
7 Mehrvarz S., Chaichi M.R., AlikhaniH.A.Effects of Phosphate Solubilizing Microorganisms and Phosphorus Chemical Fertilizeron Yield and Yield Components of Barely (Hordeum vulgare L.) //American-Eurasian J. Agric. & Environ. Sci. – 2008. – Vol. 3(6). – P. 822-828.
8 Pérez-García A., Romero D., de Vicente A. Plant protection and growth stimulation by mi-croorganisms:biotechnological application of Bacillusin agriculture // Curr. Opin. Biotechnol. – 2011. – Vol. 22. – P. 1-7.
9 Yasmin H., Bano A. Isolation and characterization of phosphate solubilizing bacteria from rhizosphere soil of weeds of khewra salt range and attock // Pakistan Journal of Botany. – 2011. – №3. – P.1663-1668.
10 Khan A.A, Jilani G., Akhtar M.S., Naqvi S., Rasheed M. Phosphorus Solubilizing Bacteria: Occurrence, Mechanisms and their Role in Crop Production // J. Agric. Biol. Sci. 2009. –Vol.1(1). – P.
48-58.
11 Vahed H.S., Shahinrokhsar P., Heydarnezhad F. Performance of phosphate solubilizing bacteriafor improving growth and yield of rice (Oryza Sativa L.) in the presence of phosphorus fer-tilizer// Int. J. Agri. Crop Sci. – 2012. – Vol. 4(17). – P. 1228- 1232.
12 Nautiyal C.S. An efficient microbiological growth medium for screening phosphate solubil-izing microorganisms // FEMS Microbiol Lett. – 1999. – Vol. 170(1). – P. 265-270.
13 Sjegi J. Metody pochvennoj mikrobiologii. – 1983. – M.: Kolos. –162 p.
Downloads
Issue
Section
MICROBIOLOGY
