Research results of artificial water systems of hotel complexes in almaty for contamination of Legionella Pneumophila
DOI:
https://doi.org/10.26577//eb.2020.v83.i2.07Abstract
Legionellosis is a sapronous acute infectious disease, with a mortality rate of about 10% in the pulmonary form, caused by various types of microorganisms belonging to the genus Legionella. According to current data, about 90% of legionellosis is associated with the species L. pneumophila. The main habitat of Legionella is abiotic environmental objects. Legionella in the majority of cases is sown from respiratory therapy equipment, air conditioning fluids, home and industrial cooling systems, shower and boiler installations. Observations have shown that the incidence of legionellosis in travelers is slightly higher than in people who do not change their location.
The purpose of this work was to study the contamination of L. pneumophila in artificial water systems of four hotels in Almaty. Flushes from various objects of water systems and water samples were selected as the material for analysis. Bacteriological studies of the samples did not yield positive results. A PCR study of 30 samples revealed 4 positive samples in one of the hotels. It was found that the water temperature in the hot water system does not exceed 40-60°C all year round. The optimal temperature in water use systems for bacterial growth is 25-60°C.
Key words: Legionellosis, Legionella, PCR, gene sections, bacteriology, molecular genetic method.
References
1. Ryan K.J., Ray C.G. «Sherris Medical Microbiology» // McGraw Hill, 2003. – С. 992. – ISBN 0-838585-29-9;
2. Swanson M., Heuner K. «Legionella: Molecular Microbiology» // Caister Academic Pr, 2008. – С. 249. – ISBN 1-904455-26-3;
3. Chee C.E., Baddour L.M. «Legionella maceachernii soft tissue infection» // Lippincott Williams & Wilkins the American journal of the medical sciences. 2007. – Vol. 334, no. 5. – P. 410-413;
4. Kubota M., Tomii K., Tachikawa R., Harada Y., Seo R., Kaji R., Takeshima Y., Hayashi M., Nishimura T., Ishihara K. «Legionella longbeachae pneumonia infection from home garden soil» / Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society. – Japan, 2007. – Vol. 45, no. 9. – P. 698-703;
5. Phares C.R., Wangroongsarb P., Chantra S., Paveenkitiporn W., Tondella M.L., Benson R.F., Thacker W.L., Fields B.S., Moore M.R., Fischer J., Dowell S.F., Olsen S.J. «Epidemiology of severe pneumonia caused by Legionella longbeachae, Mycoplasma pneumoniae, and Chlamydia pneumonia: population-based surveillance for severe pneumonia in Thailand» // The University of Chicago Press Clinical infectious diseases: an official publication of the Infectious Diseases Society of America. – USA, 2007. – No. 45. – P. 147-155;
6. Mathys W., Stanke J., Harmuth M., Junge-Mathys E. «Occurrence of Legionella in hot water systems of single-family residences in suburbs of two German cities with special reference to solar and district heating» // International journal of hygiene and environmental health. – Germany, 2008. – Vol. 211, no. 1-2. – P. 179-185;
7. Pozdeyev O.K. Glava 20. «Prikhotlivyye aerobnyye gramotritsatelnyye palochki i kokkobakterii» // Meditsinskaya mikrobiologiya: uchebnoye posobiye / pod red. V.I. Pokrovskogo. – 4-e izd. – M.: GEOTAR-Media. 2006. – S. 400. – 768 s. – 3000 ekz. – ISBN 5-9704-0132-3;
8. Osterholm M.T., Chin T.D., Osborne D.O., Dull H.B., Dean A.G., Fraser D.W., Hayes P.S., Hall W.N. A 1957 outbreak of Legionnaires' disease associated with a meat packing plant // Oxford University Press American journal of epidemiology. – USA, 1983. – No. 117. – P. 60-67;
9. Thacker S.B., Bennett J.V., Tsai T.F., Fraser D.W., McDade J.E., Shepard C.C., Williams K.H., Stuart W.H., Dull H.B., Eickhoff T.C. An outbreak in 1965 of severe respiratory illness caused by the Legionnaires' disease bacterium. // University of Chicago Press The Journal of infectious diseases. – USA, 1978. – Vol. 138, no. 4. – P. 512-519;
10. Terranova W., Cohen M.L., Fraser D.W. 1974 outbreak of Legionnaires' Disease diagnosed in 1977. Clinical and epidemiological features / Lancet Publishing Group Lancet. – Great Britain, 1978. – Vol. 2, no. 8081. – P. 122-124;
11. Tartakovskiy I.S., Sinopalnikov A.I. Legionellez: rol v infektsionnoy patologii cheloveka (rus.) // Mezhregionalnaya assotsiatsiya po klinicheskoy mikrobiologii i antimikrobnoy khimioterapii i Nauchno-issledovatelskiy institut antimikrobnoy khimioterapii SGMA Klinicheskaya mikrobiologiya i antimikrobnaya khimioterapiya. – M.: Izdatelskiy dom «M-Vesti». 2001. – T. 3. № 1;
12. Pancer K., Stypulkowska-Misiurewicz H. Pontiac fever – non-pneumonic legionellosis. // Panstwowy Zaklad Wydawnictw Lekarskich Przegla̧d epidemiologiczny. – Poland, 2003. – T. 57, nr 4. – S. 607-612;
13. Chuchalin A.G., Sinopalnikov A.I., Tartakovskiy I.S., Karpova T.I., Dronina Yu.E., Sadretdinova O.V., Kozlov R.S., Bobyleva Z.D., Leshchenko I.V., Mikhaylova D.O., Rachina S.A. Prakticheskiy rekomendatsii po diagnostike i lecheniyu legionellezoy infektsii. vyzyvayemoy Legionella pneumophila serogruppy 1. – Moskva: Rossiyskoye respiratornoy obshchestvo. Mezhregionelnaya assotsiatsiya po klinicheskoy mikrobiologii i antimikrobnoy khimioterapii. 2010. – S. 5;
14. Qasem J.A., Mustafa A.S., Khan Z.U. Legionella in clinical specimens and hospital water supply facilities: molecular detection and genotyping of the isolates // Karger Medical principles and practice: international journal of the Kuwait University, Health Science Centre. – Switzerland, 2008. – Vol. 17, no. 1. – P. 49-55;
15. Zuyeva L.P., Yafayev R.Kh. Glava 21. «Obshchaya kharakteristika sapronozov». // Epidemiologiya. – SPb.: Foliant. 2005. – S. 556. – 752 s. – 3000 ekz. – ISBN 5-93929-111-2;
16. Lisukova T., Chekalina K. «Legionellez» // Sestrinskoye delo. 2000. – № 6;
17. de Olalla P.G., Gracia J., Rius C., Caylà J.A., Pañella H., Villabí J.R., Guix J., Pellicer T., Ferrer D., Cusi M., Pelaz C., Sabrià M.; «Grupo de trabajo del brote de Vallcarca. Community outbreak of pneumonia due to Legionella pneumophila: importance of monitoring hospital cooling towers = La infectología en Europa y América». // Enfermedades infecciosas y microbiología clínica. 2008. – V. 26, no 1. – P. 15-22;
18. «Surveillance for Travel-Associated Legionnaires Disease» – United States, 2005-2006. // Centers for Disease Control and Prevention. 2007. – Vol. 56, no. 48. – P. 1261-1263;
19. Berdal B.P., editor. Legionella infections and atypical pneumonias. Proceedings of the 11th Meeting of the European Working Group on Legionella infections; 1996. – P. 87-92;
20. Rota M.C., Cano Portero R., Che D., Caporali M.G., Hernando V., Campese C. Clusters of travel-associated Legionnaires disease in Italy // European Centre for Disease Prevention and Control Euro surveillance: bulletin européen sur les maladies transmissibles = European communicable disease bulletin. – Sweden, 2007. – Vol. 12, no. 11. – P. 3-4;
21. Joseph C.A., Yadav R., Ricketts K.D.; European Working Group for Legionella Infections. Travel-associated Legionnaires disease in Europe in 2007. // European Centre for Disease Prevention and Control Euro surveillance: bulletin européen sur les maladies transmissibles = European communicable disease bulletin. – Sweden, 2009. – Vol. 14, no. 18. – P. 1-5;
22. Finley Bret. Boyevyye iskusstva bakteriy (rus.) // V mire nauki. – Rossiya: ZAO «V mire nauki». 2010. – № 4. – S. 48-49. – ISSN 0208-0621;
23. Hawn T.R., Berrington W.R., Smith I.A., Uematsu S., Akira S., Aderem A., Smith K.D., Skerrett S.J. Altered inflammatory responses in TLR5-deficient mice infected with Legionella pneumophila. // The Journal of Immunology. – USA, 2007. – Vol. 179, no. 10. – P. 6981-6987. – ISSN 0022-1767;
24. «Epidemiologicheskiy nadzor za legionelleznoy infektsiyey: Metodicheskiye ukazaniya» – M.: Federalnyy tsentr gigiyeny i epidemiologii Rospotrebnadzora. 2009. – S. 35;
25. «Profilaktika legionelleza: Sanitarno-epidemiologicheskiye pravila» – M.: Federalnyy tsentr gigiyeny i epidemiologii Rospotrebnadzora. 2010. – S. 22.
2. Swanson M., Heuner K. «Legionella: Molecular Microbiology» // Caister Academic Pr, 2008. – С. 249. – ISBN 1-904455-26-3;
3. Chee C.E., Baddour L.M. «Legionella maceachernii soft tissue infection» // Lippincott Williams & Wilkins the American journal of the medical sciences. 2007. – Vol. 334, no. 5. – P. 410-413;
4. Kubota M., Tomii K., Tachikawa R., Harada Y., Seo R., Kaji R., Takeshima Y., Hayashi M., Nishimura T., Ishihara K. «Legionella longbeachae pneumonia infection from home garden soil» / Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society. – Japan, 2007. – Vol. 45, no. 9. – P. 698-703;
5. Phares C.R., Wangroongsarb P., Chantra S., Paveenkitiporn W., Tondella M.L., Benson R.F., Thacker W.L., Fields B.S., Moore M.R., Fischer J., Dowell S.F., Olsen S.J. «Epidemiology of severe pneumonia caused by Legionella longbeachae, Mycoplasma pneumoniae, and Chlamydia pneumonia: population-based surveillance for severe pneumonia in Thailand» // The University of Chicago Press Clinical infectious diseases: an official publication of the Infectious Diseases Society of America. – USA, 2007. – No. 45. – P. 147-155;
6. Mathys W., Stanke J., Harmuth M., Junge-Mathys E. «Occurrence of Legionella in hot water systems of single-family residences in suburbs of two German cities with special reference to solar and district heating» // International journal of hygiene and environmental health. – Germany, 2008. – Vol. 211, no. 1-2. – P. 179-185;
7. Pozdeyev O.K. Glava 20. «Prikhotlivyye aerobnyye gramotritsatelnyye palochki i kokkobakterii» // Meditsinskaya mikrobiologiya: uchebnoye posobiye / pod red. V.I. Pokrovskogo. – 4-e izd. – M.: GEOTAR-Media. 2006. – S. 400. – 768 s. – 3000 ekz. – ISBN 5-9704-0132-3;
8. Osterholm M.T., Chin T.D., Osborne D.O., Dull H.B., Dean A.G., Fraser D.W., Hayes P.S., Hall W.N. A 1957 outbreak of Legionnaires' disease associated with a meat packing plant // Oxford University Press American journal of epidemiology. – USA, 1983. – No. 117. – P. 60-67;
9. Thacker S.B., Bennett J.V., Tsai T.F., Fraser D.W., McDade J.E., Shepard C.C., Williams K.H., Stuart W.H., Dull H.B., Eickhoff T.C. An outbreak in 1965 of severe respiratory illness caused by the Legionnaires' disease bacterium. // University of Chicago Press The Journal of infectious diseases. – USA, 1978. – Vol. 138, no. 4. – P. 512-519;
10. Terranova W., Cohen M.L., Fraser D.W. 1974 outbreak of Legionnaires' Disease diagnosed in 1977. Clinical and epidemiological features / Lancet Publishing Group Lancet. – Great Britain, 1978. – Vol. 2, no. 8081. – P. 122-124;
11. Tartakovskiy I.S., Sinopalnikov A.I. Legionellez: rol v infektsionnoy patologii cheloveka (rus.) // Mezhregionalnaya assotsiatsiya po klinicheskoy mikrobiologii i antimikrobnoy khimioterapii i Nauchno-issledovatelskiy institut antimikrobnoy khimioterapii SGMA Klinicheskaya mikrobiologiya i antimikrobnaya khimioterapiya. – M.: Izdatelskiy dom «M-Vesti». 2001. – T. 3. № 1;
12. Pancer K., Stypulkowska-Misiurewicz H. Pontiac fever – non-pneumonic legionellosis. // Panstwowy Zaklad Wydawnictw Lekarskich Przegla̧d epidemiologiczny. – Poland, 2003. – T. 57, nr 4. – S. 607-612;
13. Chuchalin A.G., Sinopalnikov A.I., Tartakovskiy I.S., Karpova T.I., Dronina Yu.E., Sadretdinova O.V., Kozlov R.S., Bobyleva Z.D., Leshchenko I.V., Mikhaylova D.O., Rachina S.A. Prakticheskiy rekomendatsii po diagnostike i lecheniyu legionellezoy infektsii. vyzyvayemoy Legionella pneumophila serogruppy 1. – Moskva: Rossiyskoye respiratornoy obshchestvo. Mezhregionelnaya assotsiatsiya po klinicheskoy mikrobiologii i antimikrobnoy khimioterapii. 2010. – S. 5;
14. Qasem J.A., Mustafa A.S., Khan Z.U. Legionella in clinical specimens and hospital water supply facilities: molecular detection and genotyping of the isolates // Karger Medical principles and practice: international journal of the Kuwait University, Health Science Centre. – Switzerland, 2008. – Vol. 17, no. 1. – P. 49-55;
15. Zuyeva L.P., Yafayev R.Kh. Glava 21. «Obshchaya kharakteristika sapronozov». // Epidemiologiya. – SPb.: Foliant. 2005. – S. 556. – 752 s. – 3000 ekz. – ISBN 5-93929-111-2;
16. Lisukova T., Chekalina K. «Legionellez» // Sestrinskoye delo. 2000. – № 6;
17. de Olalla P.G., Gracia J., Rius C., Caylà J.A., Pañella H., Villabí J.R., Guix J., Pellicer T., Ferrer D., Cusi M., Pelaz C., Sabrià M.; «Grupo de trabajo del brote de Vallcarca. Community outbreak of pneumonia due to Legionella pneumophila: importance of monitoring hospital cooling towers = La infectología en Europa y América». // Enfermedades infecciosas y microbiología clínica. 2008. – V. 26, no 1. – P. 15-22;
18. «Surveillance for Travel-Associated Legionnaires Disease» – United States, 2005-2006. // Centers for Disease Control and Prevention. 2007. – Vol. 56, no. 48. – P. 1261-1263;
19. Berdal B.P., editor. Legionella infections and atypical pneumonias. Proceedings of the 11th Meeting of the European Working Group on Legionella infections; 1996. – P. 87-92;
20. Rota M.C., Cano Portero R., Che D., Caporali M.G., Hernando V., Campese C. Clusters of travel-associated Legionnaires disease in Italy // European Centre for Disease Prevention and Control Euro surveillance: bulletin européen sur les maladies transmissibles = European communicable disease bulletin. – Sweden, 2007. – Vol. 12, no. 11. – P. 3-4;
21. Joseph C.A., Yadav R., Ricketts K.D.; European Working Group for Legionella Infections. Travel-associated Legionnaires disease in Europe in 2007. // European Centre for Disease Prevention and Control Euro surveillance: bulletin européen sur les maladies transmissibles = European communicable disease bulletin. – Sweden, 2009. – Vol. 14, no. 18. – P. 1-5;
22. Finley Bret. Boyevyye iskusstva bakteriy (rus.) // V mire nauki. – Rossiya: ZAO «V mire nauki». 2010. – № 4. – S. 48-49. – ISSN 0208-0621;
23. Hawn T.R., Berrington W.R., Smith I.A., Uematsu S., Akira S., Aderem A., Smith K.D., Skerrett S.J. Altered inflammatory responses in TLR5-deficient mice infected with Legionella pneumophila. // The Journal of Immunology. – USA, 2007. – Vol. 179, no. 10. – P. 6981-6987. – ISSN 0022-1767;
24. «Epidemiologicheskiy nadzor za legionelleznoy infektsiyey: Metodicheskiye ukazaniya» – M.: Federalnyy tsentr gigiyeny i epidemiologii Rospotrebnadzora. 2009. – S. 35;
25. «Profilaktika legionelleza: Sanitarno-epidemiologicheskiye pravila» – M.: Federalnyy tsentr gigiyeny i epidemiologii Rospotrebnadzora. 2010. – S. 22.
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Abdeliyev, B. Z., Yessimseit, D. T., Abdirassilova, A. A., Kassenova, A. K., Abdel, Z. Z., Rysbekova, A. K., Atshabar, B. B., Mirzatayev, Z. K., & Chunetova, Z. Z. (2020). Research results of artificial water systems of hotel complexes in almaty for contamination of Legionella Pneumophila. Experimental Biology, 83(2), 64–71. https://doi.org/10.26577//eb.2020.v83.i2.07
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BIOTECHNOLOGY
