Influence of microalgae on the microbial composition of fishery wastewater

Authors

  • N. R. Akmukhanova al-Farabi Kazakh National University, Kazakhstan, Almaty
  • A. K. Sadvakasova al-Farabi Kazakh National University, Kazakhstan, Almaty
  • M. M. Torekhanova al-Farabi Kazakh National University, Kazakhstan, Almaty
  • M. O. Bauyenova al-Farabi Kazakh National University, Kazakhstan, Almaty
  • A. Adak al-Farabi Kazakh National University, Kazakhstan, Almaty
  • A. Karabekova al-Farabi Kazakh National University, Kazakhstan, Almaty
  • B. K. Zayadan al-Farabi Kazakh National University, Kazakhstan, Almaty

DOI:

https://doi.org/10.26577//eb.2020.v83.i2.06
        145 144

Abstract

The article presents the results of a study that examined the effect of the green microalgae strain Chlorella vulgaris Z-1 on the sanitary-microbiological state of wastewater from trout and sturgeon ponds of the Saymasay fishery. The wastewater of both ponds of fisheries was characterized by a high content of organo-mineral substances. According to the results of sanitary-bacteriological studies, the total microbial number (TMN) of water in the ponds amounted to 5,6- 6,4x 105KOE / cm3 of water, at the water quality index of 10, and the presence of aeromonads and pseudomonads was also noted. It was found that after the cultivation of the microalgae Chl vulgaris Z-1 in the wastewater of the sturgeon and trout pond, the microbiological characteristic improved significantly, the TMN decreased by 70-75%, and the water of the Saymasay fishery, with index 5, considered to be clean by evaluating the degree of pollution. It was found in the morphological composition of the microflora of both ponds that there was a noticeable decrease in the groups of rod-shaped microorganisms, an increase in the content of cocciform bacteria and the absence of aeromonads and pseudomonads. A change in the ratio of physiological groups of microorganisms in purified water after microalgae cultivation was revealed. The number of ammonifying and denitrifying bacteria noticeably decreased, while the number of nitrifying microorganisms increased significantly, which indicates the content of semi-decomposed organic residues involved in the ammonification process.

Key words: Chlorella vulgaris Z-1, TMN (total microbial number), sanitary and microbiological condition, fishery waste water.

References

1. Abedin RM, Taha HM. Antibacterial and antifungal activity of cyanobacteria and green microalgae. Evaluation of medium components by Plackett-Burman design for antimicrobial activity of Spirulina platensis. Global Journal of Biotechnology and Biochemistry. 2008;3(1):22-31.
2. Arapova A. B. Biologicheskoye udaleniye azota i fosfora iz gorodskikh stochnykh vod: dis. kand. tekhn. nauk: 03.00.16 : zashchishchena 24.12.04 // Arapova A. B. - M.2004. - 192 s.
3. Braginskiy L.P., Kraynyukova A.N. Metody otsenki toksichnosti stochnykh vod i perspektivy ikh ispol'zovanii v kontrole prirodnykh vod // Metody bioindikatsii i biotestirovaniya prirodnykh vod. L.:Gidrometeoizdat, 1989.- S.194-203.
4. Bukholder, P.R., L.M. Burkholder & I.R. Almodovar. 1960. Antibiotic activity of some marine algae of Puerto Rico. Bot. Mar. 2: 149-156.
5. Berdzhi. Opredelitel' bakteriy. M.: Mir, 1997. - 799 s.
6. Dzyuban A.N. Otsenka ekologicheskogo sostoyaniya vodokhranilishch po kriteriyam bakterio- bentosa // Gidrobiol. zhurn. – 2004. – №4. – S. 32-33.
7. Gol'din Ye.B., Gol'dina V.G. Antibakterial'nyye svoystva metabolitov vodorosley v model'nykh eksperimentakh. Al'gologiya. 1999. 2: 34.
8. GOST ISO 7218-2015 Mikrobiologiya pishchevykh produktov i kormov dlya zhivotnykh. Obshchiye trebovaniya i rekomendatsii po mikrobiologicheskim issledovaniyam
9. Guo Z, Liu Y, Guo H, Yan S and Mu J, Microalgae cultivation using anaquaculture wastewater as growth medium for biomass and biofuel production. JEnvironSci 25:S85–S88 (2013).
10. Gorobets O.B., Blinkova L.P., Baturo A.P. Vliyaniye mikrovodorosley na zhiznesposobnost' mikroorganizmov v yestestvennoy i iskusstvennoy srede obitaniya. Zhurn. mikrobiol. 2001. 1: 104-108
11. Hend A. and Perveen K. Antibacterial activity and morphological changes in human pathogenic bacteria caused by Chlorella vulgaris extracts. Biomed Res- India. 2017. (28)4. 1610-1614
12. Ignatenko M.Ye., Nemtseva N.V.. Mekhanizmy vzaimodeystviya avtotrofnogo i geterotrofnogo komponentov v al'gobakterial'nykh soobshchestvakh. Byulleten' Orenburgskogo nauchnogo tsentra UrO RAN (elektronnyy zhurnal). 2012. №3
13. Ignatenko M.Ye. Kharakteristika simbioticheskikh svyazey mikroorganizmov v al'gobakterial'nykh soobshchestvakh prirodnykh vodoyemov. Avtoref. diss. … kand. biol. nauk. Orenburg, 2008. S. 19.
14. Jaki, B., J. Helimann & O. Sticher. 2000. New antimicrobial metabolites from the cyanobacterium Nostoc commune. J. Nat. Prod. 63: 1283.
15. Metodicheskiye ukazaniya po sanitarno- bakteriologicheskoy otsenke rybokhozyaystvennykh vodoyemov №13-4-2-/1738, utverzhdennyye 27 sentyabrya 1999 g // Sbornik instruktsiy po bor'be s boleznyami ryb. M.: Otdel marketinga AMB – agro. 1999. CH. 2. C. 161–177
16. Nemtseva N.V., Selivanova Ye.A., Plotnikov A.O. Rol' simbioticheskikh vzaimodeystviy v vyzhivanii mikroorganizmov v gipergalinnykh vodoyemakh. Zhurn. mikrobiol. 2006. 4: 117120.
17. Pradhan J., Das S. and Das B. K. Antibacterial activity of freshwater microalgae: A review. African Journal of Pharmacy and Pharmacology. 2014. 8(32). 809-818
18. Pratt R., Daniel T.C., Eier J.B., Gunnison J.B., Kumler W.D., Oneto J.F., Strait L.A., Spoehr H.A., Hardin G.J, Milner H.W, Smith H., Strain H.H. Chlorellin. An antibacterial substance from chlorella. Science. 1944. 99. 351-352
19. Rekomendatsii po metodam proizvodstva analizov na sooruzheniyakh biokhimicheskoy ochistki promyshlennykh stochnykh vod. - M., 1970.
20. Syed Sh., Arasu A. and Ponnuswamy I. The Uses of Chlorella Vulgaris as Antimicrobial Agent and as a Diet: the Presence of Bioactive Compounds which caters the Vitamins, Minerals in General. International Journal of Bio-Science and Bio-Technology. 2015. 7(1). 185-190.
21. Scott A.C. Laboratory control of antimicrobial therapy. In: Mackie & McCartney Practical medical microbiology (Edited by, Collee JG, Duguid JP, Fraser AG and Marmion BP) 13th Edn. Vol 2. United Kingdom-Edinburgh: Churchill Livingstone. 1989. 161-181.
22. Zayadan B.K., Sadvakasova A.K., Akmukhanova N.R. Biotechnology of phototrophic microorganisms. Almaty: "Kazakh University", 2019. -350.
23. Zayadan B.K., Sadvakasova A.K., Akmukhanova N.R. Laboratornaya praktika po biotekhnologii fototrofnykh mikroorganizmov // Uchebnik Kazakhskogo universiteta, 2018, -262 s. ISBN 978-601-04-3133-1
24. B.K. Zayadan, A.K. Sadvakasova, D.K. Kirbayeva, K. Bolatkhan,M. Salekh, M. Bauyenova. Bezotkhodnaya tekhnologiya biologicheskoy ochistki stochnykh vod s pomoshch'yu mikrovodorosley// Kaznu Bulletin. Ecology series. №2/2 (38). 2013
25. Zayadan B.K., Userbayeva A., Bolatkhan K., Sarsekeyeva F., Sadvakasova A.K. Bezotkhodnaya tekhnologiya ochistki stochnykh vod s polucheniyem biodizel'nogo topliva na osnove shtamma mikrovodorosli - produtsenta masla. Vestnik KazNU. Seriya biologicheskaya. №3/1(59). 2013. C. 100–103
26. Zenova G.M., Shtina E.A., Dedysh S.N. i dr. Ekologicheskiye svyazi vodorosley v biotsenozakh. Mikrobiologiya. 1995. 2: 149 - 164.

Downloads

How to Cite

Akmukhanova, N. R., Sadvakasova, A. K., Torekhanova, M. M., Bauyenova, M. O., Adak, A., Karabekova, A., & Zayadan, B. K. (2020). Influence of microalgae on the microbial composition of fishery wastewater. Experimental Biology, 83(2), 53–63. https://doi.org/10.26577//eb.2020.v83.i2.06

Issue

Vol. 83 No. 2 (2020): Вестник КазНУ. Серия биологическая

Section

BIOTECHNOLOGY

Most read articles by the same author(s)

1 2 > >>