Optimization of the conditions of ELISA for the diagnosis of FMD virus type A
DOI:
https://doi.org/10.26577/eb-2019-1-1411Abstract
By concentration of PEG-6000 and purification with chloroform, strain "Talasskiy" of FMD virus type A is prepared with purified antigens, the activity of which was in CBR 1:32-1:64. For diagnostic sera antigens were subjected to additional purification after 30% sucrose cushion at 35,000 rpm for 2 hours. After purification, the antigen activity was 1:16-1: 32. Using purified antigen of FMD virus type A antiserums on sheep, pigs and rabbits with activity 1:8-1:64 were obtained. From antisera by Kohn isolated immunoglobulin, whose activity in the DPR was 1: 32, and the protein concentration ranging from 41.84 mg / ml to 60.2 mg / ml.
Periodate using the method of M. B. Wilson, P. K. Nakane on the basis of specific porcine globulin conjugate prepared to optimize the conditions of production ELISA.
The prepared diagnostic preparations are used to optimize the conditions for the formulation of ELISA for the diagnosis of FMD type A.
The optimized version of ELISA proved to be sensitive and effective in the study of heterogeneous and homogeneous biomaterials.
Key words: FMD type A, immunoassay, immunoglobulin, immunization, antigen
References
1 Reid S.M., Ferris N.P., Hutchings G.H., Zhang Z., Belsham G.J., Alexandersen S. Detection of all seven serotypes of footand-mouth
disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay // J. Virol. Meth. – 2002.
– Vol. 105. – PР. 67-80.
2 Foot And Mouth Disease Virus/Manual of Standards for Diagnostic Tests and Vaccines – Paris: OIE.- 1996.- РР. 47 – 57.
3 Kitching R. P. Barnett P. V. Donaldson A. I. Mackay D. K. J. Foot and mouth disease. Manual of standards for diagnostic
tests and vaccines: Lists A and B diseases of mammals, birds and bees Paris: OIE.- 2001.- РР. 77-92.
4 Nagendrakumar S.B., Reddy G.S., Chandran D., Thiagarajan D., Rangarajan P.N., Srinivasan V.A. Molecular characterization
of foot-and-mouth disease virus type C of Indian origin // J. Clinical Microbiol. – 2005. – Vol. 43. N. 2. – PР. 966-969.
5 Have P., Lei J. C., Schjerning-Thiesen K. “An engyme linked immunosorbent assay (ELISA) for the primary diagnosis of
foot-and-mouth disease: characterization and comparison with complement fixation” // Acta Veterinaria Scandinavica. Supplementum.
– Vol. 25. – No. 2. – PP. 280–296. – 1984.
6 Ferris N. P., Dawson M. “Routine application of enzyme-linked immunosorbent assay in comparison with complement fixation
for the diagnosis of foot-and-mouth and swine vesicular diseases” // Veterinary Microbiology. – Vol. 16. – No. 3. – PP. 201–209.
– 1988.
7 Abu Elzein E. M. E., Crowther J. R. “Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus
research” // Journal of Hygiene. – Vol. 80. – No. 3. – PP. 391–399. – 1978.
8 Crowther J. R., Abu Elzein E. M. E. “Application of the enzyme linked immunosorbent assay to the detection and identification
of foot-and-mouth disease viruses” // Journal of Hygiene. – Vol. 83. – No. 3. – PP. 513–519. – 1979.
9 Ouldridge E. J., Barnett P., Rweyemamu M. M. “The relative efficiency of two ELISA techniques for the tritration of FMD
antigen” // Current Topics in Veterinary Medicine and Animal Science. – Vol. 22. – PP. 142–151. – 1982.
10 Abu Elzein E. M. E., J. R. Crowther. “The specific detection of foot-and-mouth disease virus whole particle antigen (140S)
by enzyme labelled immunosorbent assay” // Journal of Hygiene. – Vol. 83. – No. 1. – PP. 127–134. – 1979.
11 Rai A., Lahiri D. K. “A micro-enzyme-labelled immunosorbent assay (MICROELISA) for the detection of foot-and-mouth
disease virus antigen and antibody” // Acta Virologica. – Vol. 25. – No. 1. – PP. 49–52. – 1981.
12 Hamblin C., Armstrong R. M., Hedger R. S. “A rapid enzyme-linked immunosorbent assay for the detection of foot-andmouth
disease virus in epithelial tissues” // Veterinary Microbiology. – Vol. 9. – No. 5. – PP. 435–443. – 1984.
13 Ouldridge E. J., Barnett P. V., Hingley J., Rweyemamu M. M. “The differentiation of foot and mouth disease virus strains
using an indirect sandwich enzyme-linked immunosorbent assay saturation model” // Journal of Biological Standardization. – Vol.
12. – No. 4. – PP. 367–377. – 1984.
14 Roeder P. L., Le Blanc Smith P. M. “Detection and typing of foot-and-mouth disease virus by enzyme-linked immunosorbent
assay: a sensitive, rapid and reliable technique for primary diagnosis” // Research in Veterinary Science. – vol. 43. – No. 2. – PP.
225–232. – 1987.
15 Pattnaik B., Venkataramanan R. “Indirect enzyme linked immunosorbent assay for the detection of FMDV antigen” // Indian
Journal of Animal Sciences. – Vol. 59. – PP. 317–322. – 1989.
16 Pattnaik B., Venkataramanan R. “A sandwich enzyme linked immunosorbent assay for subtype analysis of foot-and-mouth
disease virus isolate” // Indian Journal of Animal Sciences. – Vol. 59. – PP. 1363–1368. – 1989.
17 Сюрин В.Н., Белоусова Р.В, Фомина Н.В. Диагностика вирусных болезней животных // М., "Агропромиздат"- 1991г.
– С. 434 – 463.
18 Paiba G. A., Anderson J., Paton D. J. “Validation of a foot-and-mouth disease antibody screening solid-phase competition
ELISA (SPCE)” // Journal of Virological Methods. – Vol. 115. – No. 2. – PP. 145–158. – 2004.
19 Bhattacharya S., Pattnaik B., Venkataramanan R. “Development and application of a sandwich enzyme-linked immunosorbent
assay (ELISA) for type identification of foot-and-mouth-disease (FMD) virus in direct field materials” // Indian Journal of
Animal Sciences. -Vol. 66. – No. 12. – PP. 1201–1209. – 1996.
20 Grubman M. J., Baxt B. “Foot-and-mouth disease” // Clinical Microbiology Reviews. – Vol. 17. – No. 2. – PP. 465–493. –
2004.
21 Мищенко В.А. Иммуноферментный метод в диагностике ящура // Вопр. вирусол. – 1991. – №4. – С. 341 -342.
22 Cohn E., Strong L., Hughes W. Fractionation of proteins from plasma //J. Amer. Chem. Soc.- 1946. – V.68. – 459 р.
23 Oncley J., Melin M., Richert D. The separation of the antibodies //J. Amer. Chem. Soc., 1949. – V.71. – PР. 541-550.
24 Kistler P., Nitschman H. Production of proteins from plasma // Vox. Sang., 1962. – V.7. – P. 414– 424.
25 Мищенко В.А., Шажко Ж.А., Базаров М.А., Конюшкина Т.Б., Смирнов А.Б., Атаев Э.А. Сравнительная оценка
методов конъюгации антител к вирусу ящура с пероксидазой хрена // Акт. пробл. вет. вирусологии. Тез. докл. науч. конф.
ВНИЯИ, – Владимир, 1987.- 2.- С. 49 – 50.
26 Щербаков А.В. Молекулярная эпизоотология ящура в России (филогенетический анализ российских изолятов
вируса ящура) // Ветеринария сегодня. 2015.-(3).- С. 30-36.
disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay // J. Virol. Meth. – 2002.
– Vol. 105. – PР. 67-80.
2 Foot And Mouth Disease Virus/Manual of Standards for Diagnostic Tests and Vaccines – Paris: OIE.- 1996.- РР. 47 – 57.
3 Kitching R. P. Barnett P. V. Donaldson A. I. Mackay D. K. J. Foot and mouth disease. Manual of standards for diagnostic
tests and vaccines: Lists A and B diseases of mammals, birds and bees Paris: OIE.- 2001.- РР. 77-92.
4 Nagendrakumar S.B., Reddy G.S., Chandran D., Thiagarajan D., Rangarajan P.N., Srinivasan V.A. Molecular characterization
of foot-and-mouth disease virus type C of Indian origin // J. Clinical Microbiol. – 2005. – Vol. 43. N. 2. – PР. 966-969.
5 Have P., Lei J. C., Schjerning-Thiesen K. “An engyme linked immunosorbent assay (ELISA) for the primary diagnosis of
foot-and-mouth disease: characterization and comparison with complement fixation” // Acta Veterinaria Scandinavica. Supplementum.
– Vol. 25. – No. 2. – PP. 280–296. – 1984.
6 Ferris N. P., Dawson M. “Routine application of enzyme-linked immunosorbent assay in comparison with complement fixation
for the diagnosis of foot-and-mouth and swine vesicular diseases” // Veterinary Microbiology. – Vol. 16. – No. 3. – PP. 201–209.
– 1988.
7 Abu Elzein E. M. E., Crowther J. R. “Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus
research” // Journal of Hygiene. – Vol. 80. – No. 3. – PP. 391–399. – 1978.
8 Crowther J. R., Abu Elzein E. M. E. “Application of the enzyme linked immunosorbent assay to the detection and identification
of foot-and-mouth disease viruses” // Journal of Hygiene. – Vol. 83. – No. 3. – PP. 513–519. – 1979.
9 Ouldridge E. J., Barnett P., Rweyemamu M. M. “The relative efficiency of two ELISA techniques for the tritration of FMD
antigen” // Current Topics in Veterinary Medicine and Animal Science. – Vol. 22. – PP. 142–151. – 1982.
10 Abu Elzein E. M. E., J. R. Crowther. “The specific detection of foot-and-mouth disease virus whole particle antigen (140S)
by enzyme labelled immunosorbent assay” // Journal of Hygiene. – Vol. 83. – No. 1. – PP. 127–134. – 1979.
11 Rai A., Lahiri D. K. “A micro-enzyme-labelled immunosorbent assay (MICROELISA) for the detection of foot-and-mouth
disease virus antigen and antibody” // Acta Virologica. – Vol. 25. – No. 1. – PP. 49–52. – 1981.
12 Hamblin C., Armstrong R. M., Hedger R. S. “A rapid enzyme-linked immunosorbent assay for the detection of foot-andmouth
disease virus in epithelial tissues” // Veterinary Microbiology. – Vol. 9. – No. 5. – PP. 435–443. – 1984.
13 Ouldridge E. J., Barnett P. V., Hingley J., Rweyemamu M. M. “The differentiation of foot and mouth disease virus strains
using an indirect sandwich enzyme-linked immunosorbent assay saturation model” // Journal of Biological Standardization. – Vol.
12. – No. 4. – PP. 367–377. – 1984.
14 Roeder P. L., Le Blanc Smith P. M. “Detection and typing of foot-and-mouth disease virus by enzyme-linked immunosorbent
assay: a sensitive, rapid and reliable technique for primary diagnosis” // Research in Veterinary Science. – vol. 43. – No. 2. – PP.
225–232. – 1987.
15 Pattnaik B., Venkataramanan R. “Indirect enzyme linked immunosorbent assay for the detection of FMDV antigen” // Indian
Journal of Animal Sciences. – Vol. 59. – PP. 317–322. – 1989.
16 Pattnaik B., Venkataramanan R. “A sandwich enzyme linked immunosorbent assay for subtype analysis of foot-and-mouth
disease virus isolate” // Indian Journal of Animal Sciences. – Vol. 59. – PP. 1363–1368. – 1989.
17 Сюрин В.Н., Белоусова Р.В, Фомина Н.В. Диагностика вирусных болезней животных // М., "Агропромиздат"- 1991г.
– С. 434 – 463.
18 Paiba G. A., Anderson J., Paton D. J. “Validation of a foot-and-mouth disease antibody screening solid-phase competition
ELISA (SPCE)” // Journal of Virological Methods. – Vol. 115. – No. 2. – PP. 145–158. – 2004.
19 Bhattacharya S., Pattnaik B., Venkataramanan R. “Development and application of a sandwich enzyme-linked immunosorbent
assay (ELISA) for type identification of foot-and-mouth-disease (FMD) virus in direct field materials” // Indian Journal of
Animal Sciences. -Vol. 66. – No. 12. – PP. 1201–1209. – 1996.
20 Grubman M. J., Baxt B. “Foot-and-mouth disease” // Clinical Microbiology Reviews. – Vol. 17. – No. 2. – PP. 465–493. –
2004.
21 Мищенко В.А. Иммуноферментный метод в диагностике ящура // Вопр. вирусол. – 1991. – №4. – С. 341 -342.
22 Cohn E., Strong L., Hughes W. Fractionation of proteins from plasma //J. Amer. Chem. Soc.- 1946. – V.68. – 459 р.
23 Oncley J., Melin M., Richert D. The separation of the antibodies //J. Amer. Chem. Soc., 1949. – V.71. – PР. 541-550.
24 Kistler P., Nitschman H. Production of proteins from plasma // Vox. Sang., 1962. – V.7. – P. 414– 424.
25 Мищенко В.А., Шажко Ж.А., Базаров М.А., Конюшкина Т.Б., Смирнов А.Б., Атаев Э.А. Сравнительная оценка
методов конъюгации антител к вирусу ящура с пероксидазой хрена // Акт. пробл. вет. вирусологии. Тез. докл. науч. конф.
ВНИЯИ, – Владимир, 1987.- 2.- С. 49 – 50.
26 Щербаков А.В. Молекулярная эпизоотология ящура в России (филогенетический анализ российских изолятов
вируса ящура) // Ветеринария сегодня. 2015.-(3).- С. 30-36.
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How to Cite
Nurabaev, S. S., Volgin, E. S., Orazaliev, D. M., Ismagambetov, B. M., Kondibaeva, Z. B., Orazymbetova, N. K., Sapargalieva, N. S., Zakar’ja, K. D., & Koshemetov, Z. K. (2019). Optimization of the conditions of ELISA for the diagnosis of FMD virus type A. Experimental Biology, 78(1), 76–86. https://doi.org/10.26577/eb-2019-1-1411
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