Biochemical and immunological properties of excretory-secretory antigen Opisthorchis felineus
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Keywords:
immunology, Opisthorchis felineus, a marita, excretory-secretory antigen, electrophoresis, immunogenicity.Abstract
The main component at statement of serological reactions is an antigen, which should have the necessary biochemical and immunological properties, high specificity and activity. The article presents data on the production of excretory-secretory antigens of the Opisthorchis felineus pathogen. Describes a scheme for obtaining an antigenic product which is obtained by culturing Opisthorchis pathogens in vitro in the RPMI-1640 synthetic medium and further purified by centrifugation. Comparable experiments for the detection of protein antigen components show advantage silver staining method over the standard method, which is based on using Coomassie solution. Considering this fact, by its author has been proven that in the excretory-secretory antigen includes up to 21 of the protein fraction. The article presents data on the study of activity, specificity and immunogenicity of excretory-secretory antigen. Using the antigen in indirect ELISA version with sera of infected dogs showed its high activity and the possibility of applying for the detection of specific antibodies against the opisthorchiasis pathogen. In addition, the use of said antigen for immunization of laboratory mice and Syrian hamsters showed high immunological activity of antigen and ability to induce an immune response in the organism of laboratory animals.
References
Литература
1. Wongratanacheewin S., Sermswan R.W., Sirisinha S. Immunology and molecular biology of Opisthorchis viverrini infection // Acta Trop. – 2003. – Vol. 88(3). – P. 195-207. DOI:10.1016/j.actatropica.2003.02.002
2. Sun T., Gibson J.B. Antigens of Clonorchis sinensis in experimental and human infections // Am. J. Trop. Med. Hyg. – 1969. – Vol. 18. – P. 241-252.
3. Sun T., Gibson J.B. Metabolic products of adult Clonorchis sinensis: their composition and antigenic potential // J. Helminthol. – 1969. – Vol. 43. P. 395-402.
4. Kojima S., Yokogawa M., Tada T. Production and properties of reaginic antibodies in rabbits infected with Clonorchis sinensis or Schistosoma japonicum // Exp. Parasitol. – 1974. – Vol. 35. – P. 141-149.
5. Chen C.Y., Hsieh W.C., Shih H.H., Chen S.N. Immunodiagnosis of clonorchiasis by enzyme-linked immunosorbent assay // Southeast Asian J. Trop. Med. Publ. Health. – 1988. – Vol. 19. – P. 117-121.
6. Klebanovskaia I.A. Indirect hemagglutination reaction in the diagnosis of the early phase of opisthorchiasis. I. A method of isolating erythrocyte samples and the results of their diagnostic use in experimental opisthorchiasis in golden hamsters // Med. Parazitol. (Mosk). – 1981. – Vol. 50. – P. 20-23.
7. Klebanovskaia I.A. Indirect hemagglutination reaction in the diagnosis of the early phase of opisthorchiasis. 3. An economic method of obtaining a dried, erythrocyte opisthorchid antigenic diagnostic agent // Med. Parazitol. (Mosk). – 1985. – Vol. 54. – P. 25-27.
8. Glupov V.V., Khokhlova N.I., Khvoshchevskaia M.F., Vodianitskaia S.N., Iurlova, N.I. The use of immunoblotting for studying Opisthorchis felineus (Rivolta, 1884) antigens // Med. Parazitol. (Mosk). – 1997. – Vol. 66. – P. 17-19.
9. Kotelkin A.T., Razumov I.A., Pokrovskii I.V., Loktev V.B. A comparative study of the somatic, excretory-secretory and egg antigens of Opisthorchis felineus // Med. Parazitol. (Mosk). – 1997. – Vol. 66. – P. 12-16.
10. Meniavtseva T.A., Ratner G.M., Struchkova S.V., Kolmakova M.V., Stepanova T.F., Lepekhin A.V., Maier V.A., Postnikova T.F. Immunoenzyme analysis in the diagnosis of opisthorchiasis. I. The development of an immunoenzyme method for determining IgM antibodies to the Opisthorchis antigen // Med. Parazitol. (Mosk). – 1996. – Vol. 65. – P. 41-43.
11. Wongratanacheewin S., Sirisinha S. Analysis of Opisthorchis viverrini antigens: physicochemical characterization and antigen localization // Southeast Asian J. Trop. Med. Publ. Health. – 1987. – Vol. 18. – P. 511-520.
12. Wongratanacheewin S., Chawengkirttikul R., Bunnag D., Sirisinha S. Analysis of Opisthorchis viverrini antigens by immunoprecipitation and polyacrylamide gel electrophoresis // Parasitology. – 1988. – Vol. 96. – P. 119-128.
13. Боровиков С.Н., Куйбагаров М.А., Сураншиев Ж.А., Баешева Д.А., Атыгаева С.К., Халикова А.С. Получение и изучение иммунохимических свойств антигенов описторхов // Биотехнология. Теория и практика. – 2010. – №4. – С. 70-74.
14. Lvova M.N., Tangkawattana S., Balthaisong S., Katokhin A.V., Mordvinov V.A., Sripa B. Comparative histopathology of Opisthorchis felineus and Opisthorchis viverrini in a hamster model: an implication of high pathogenicity of the European liver fluke // Parasitol Int. – 2012. – Vol. 61(1). – P. 167-172. DOI: 10.1016/j.parint.2011.08.005.
15. Фридлянская И.И. Получение поликлональных антител // Методы культивирования клеток: Сб. науч. трудов, Л.: Наука. – 1988. – С. 194-205.
16. Киян В.С., Токпан С.С., Байболин Ж.К., Тетерин А.В., Мертасов А.Г. Гематологические показатели собак при искусственном заражении описторхозом // Вестник ПГУ. Серия Химико-биологическая, Павлодар. – 2015. – №3. – С. 12-20.
References
1. Wongratanacheewin S, Sermswan RW, Sirisinha S (2003) Immunology and molecular biology of Opisthorchis viverrini infection, Acta Trop, 88:195-207. DOI:10.1016/j.actatropica.2003.02.002
2. Sun T, Gibson JB (1969) Antigens of Clonorchis sinensis in experimental and human infections, Am. J. Trop. Med. Hyg., 18:241-252.
3. Sun T, Gibson JB (1969) Metabolic products of adult Clonorchis sinensis: their composition and antigenic potential, J. Helminthol., 43:395-402.
4. Kojima S, Yokogawa M, Tada T (1974) Production and properties of reaginic antibodies in rabbits infected with Clonorchis sinensis or Schistosoma japonicum, Exp. Parasitol., 35:141-149.
5. Chen CY, Hsieh WC, Shih HH, Chen SN (1988) Immunodiagnosis of clonorchiasis by enzyme-linked immunosorbent assay, Southeast Asian J. Trop. Med. Publ. Health., 19:117-121.
6. Klebanovskaia I.A. (1981) Indirect hemagglutination reaction in the diagnosis of the early phase of opisthorchiasis. I. A method of isolating erythrocyte samples and the results of their diagnostic use in experimental opisthorchiasis in golden hamsters, Med. Parazitol. (Mosk)., 50:20-23.
7. Klebanovskaia IA (1985) Indirect hemagglutination reaction in the diagnosis of the early phase of opisthorchiasis. 3. An economic method of obtaining a dried, erythrocyte opisthorchid antigenic diagnostic agent, Med. Parazitol. (Mosk)., 54:25-27.
8. Glupov VV, Khokhlova NI, Khvoshchevskaia MF, Vodianitskaia SN, Iurlova NI (1997) The use of immunoblotting for studying Opisthorchis felineus (Rivolta, 1884) antigens, Med. Parazitol. (Mosk)., 66:17-19.
9. Kotelkin AT, Razumov IA, Pokrovskii IV, Loktev VB (1997) A comparative study of the somatic, excretory-secretory and egg antigens of Opisthorchis felineus, Med. Parazitol. (Mosk)., 66:12-16.
10. Meniavtseva TA, Ratner GM, Struchkova SV, Kolmakova MV, Stepanova TF, Lepekhin AV, Maier VA, Postnikova TF (1996) Immunoenzyme analysis in the diagnosis of opisthorchiasis. I. The development of an immunoenzyme method for determining IgM antibodies to the Opisthorchis antigen, Med. Parazitol. (Mosk)., 65:41-43.
11. Wongratanacheewin S, Sirisinha S (1987) Analysis of Opisthorchis viverrini antigens: physicochemical characterization and antigen localization, Southeast Asian J. Trop. Med. Publ. Health., 18:511-520.
12. Wongratanacheewin S, Chawengkirttikul R, Bunnag D, Sirisinha S (1988) Analysis of Opisthorchis viverrini antigens by immunoprecipitation and polyacrylamide gel electrophoresis, Parasitology, 96:119-128.
13. Borovikov SN, Kuibagarov MA, Suranshiev ZhA, Baesheva DA, Atygaeva SK, Khalikova AS (2010) Preparation and study immunochemical properties of the opistorchis antigens [Poluchenie i izuchenie immunokhimicheskikh svoistv antigenov opistorkhov], Biothechnology. Theory and practice, 4:70-74. (In Russian)
14. Lvova MN, Tangkawattana S, Balthaisong S, Katokhin AV, Mordvinov VA, Spira B (2012) Comparative histopathology of Opisthorchis felineus and Opisthorchis viverrini in a hamster model: an implication of high pathogenicity of the European liver fluke, Parasitol Int., 61:167-172. DOI: 10.1016/j.parint.2011.08.005.
15. Fridlianskaia II (1988) Preparation the polyclonal antibody. Cell culture techniques [Poluchenie poliklonal'nykh antitel. Metody kul'tivirovaniia kletok], Bullet of scientific papers, Nauka, Leningrad, 194-205. (In Russian)
16. Kiyan VS, Tokpan SS, Baibolin ZhK, Teterin AV, Mertasov AG (2015) Hematologic indicators of the dogs under artificial infection by opisthorchiasis [Gematologicheskie pokazateli sobak pri iskusstvennom zarazhenii opistorkhozom], Bulletin of PSU. A series of chemical and biological, Pavlodar, 3:12-20. (In Russian)
1. Wongratanacheewin S., Sermswan R.W., Sirisinha S. Immunology and molecular biology of Opisthorchis viverrini infection // Acta Trop. – 2003. – Vol. 88(3). – P. 195-207. DOI:10.1016/j.actatropica.2003.02.002
2. Sun T., Gibson J.B. Antigens of Clonorchis sinensis in experimental and human infections // Am. J. Trop. Med. Hyg. – 1969. – Vol. 18. – P. 241-252.
3. Sun T., Gibson J.B. Metabolic products of adult Clonorchis sinensis: their composition and antigenic potential // J. Helminthol. – 1969. – Vol. 43. P. 395-402.
4. Kojima S., Yokogawa M., Tada T. Production and properties of reaginic antibodies in rabbits infected with Clonorchis sinensis or Schistosoma japonicum // Exp. Parasitol. – 1974. – Vol. 35. – P. 141-149.
5. Chen C.Y., Hsieh W.C., Shih H.H., Chen S.N. Immunodiagnosis of clonorchiasis by enzyme-linked immunosorbent assay // Southeast Asian J. Trop. Med. Publ. Health. – 1988. – Vol. 19. – P. 117-121.
6. Klebanovskaia I.A. Indirect hemagglutination reaction in the diagnosis of the early phase of opisthorchiasis. I. A method of isolating erythrocyte samples and the results of their diagnostic use in experimental opisthorchiasis in golden hamsters // Med. Parazitol. (Mosk). – 1981. – Vol. 50. – P. 20-23.
7. Klebanovskaia I.A. Indirect hemagglutination reaction in the diagnosis of the early phase of opisthorchiasis. 3. An economic method of obtaining a dried, erythrocyte opisthorchid antigenic diagnostic agent // Med. Parazitol. (Mosk). – 1985. – Vol. 54. – P. 25-27.
8. Glupov V.V., Khokhlova N.I., Khvoshchevskaia M.F., Vodianitskaia S.N., Iurlova, N.I. The use of immunoblotting for studying Opisthorchis felineus (Rivolta, 1884) antigens // Med. Parazitol. (Mosk). – 1997. – Vol. 66. – P. 17-19.
9. Kotelkin A.T., Razumov I.A., Pokrovskii I.V., Loktev V.B. A comparative study of the somatic, excretory-secretory and egg antigens of Opisthorchis felineus // Med. Parazitol. (Mosk). – 1997. – Vol. 66. – P. 12-16.
10. Meniavtseva T.A., Ratner G.M., Struchkova S.V., Kolmakova M.V., Stepanova T.F., Lepekhin A.V., Maier V.A., Postnikova T.F. Immunoenzyme analysis in the diagnosis of opisthorchiasis. I. The development of an immunoenzyme method for determining IgM antibodies to the Opisthorchis antigen // Med. Parazitol. (Mosk). – 1996. – Vol. 65. – P. 41-43.
11. Wongratanacheewin S., Sirisinha S. Analysis of Opisthorchis viverrini antigens: physicochemical characterization and antigen localization // Southeast Asian J. Trop. Med. Publ. Health. – 1987. – Vol. 18. – P. 511-520.
12. Wongratanacheewin S., Chawengkirttikul R., Bunnag D., Sirisinha S. Analysis of Opisthorchis viverrini antigens by immunoprecipitation and polyacrylamide gel electrophoresis // Parasitology. – 1988. – Vol. 96. – P. 119-128.
13. Боровиков С.Н., Куйбагаров М.А., Сураншиев Ж.А., Баешева Д.А., Атыгаева С.К., Халикова А.С. Получение и изучение иммунохимических свойств антигенов описторхов // Биотехнология. Теория и практика. – 2010. – №4. – С. 70-74.
14. Lvova M.N., Tangkawattana S., Balthaisong S., Katokhin A.V., Mordvinov V.A., Sripa B. Comparative histopathology of Opisthorchis felineus and Opisthorchis viverrini in a hamster model: an implication of high pathogenicity of the European liver fluke // Parasitol Int. – 2012. – Vol. 61(1). – P. 167-172. DOI: 10.1016/j.parint.2011.08.005.
15. Фридлянская И.И. Получение поликлональных антител // Методы культивирования клеток: Сб. науч. трудов, Л.: Наука. – 1988. – С. 194-205.
16. Киян В.С., Токпан С.С., Байболин Ж.К., Тетерин А.В., Мертасов А.Г. Гематологические показатели собак при искусственном заражении описторхозом // Вестник ПГУ. Серия Химико-биологическая, Павлодар. – 2015. – №3. – С. 12-20.
References
1. Wongratanacheewin S, Sermswan RW, Sirisinha S (2003) Immunology and molecular biology of Opisthorchis viverrini infection, Acta Trop, 88:195-207. DOI:10.1016/j.actatropica.2003.02.002
2. Sun T, Gibson JB (1969) Antigens of Clonorchis sinensis in experimental and human infections, Am. J. Trop. Med. Hyg., 18:241-252.
3. Sun T, Gibson JB (1969) Metabolic products of adult Clonorchis sinensis: their composition and antigenic potential, J. Helminthol., 43:395-402.
4. Kojima S, Yokogawa M, Tada T (1974) Production and properties of reaginic antibodies in rabbits infected with Clonorchis sinensis or Schistosoma japonicum, Exp. Parasitol., 35:141-149.
5. Chen CY, Hsieh WC, Shih HH, Chen SN (1988) Immunodiagnosis of clonorchiasis by enzyme-linked immunosorbent assay, Southeast Asian J. Trop. Med. Publ. Health., 19:117-121.
6. Klebanovskaia I.A. (1981) Indirect hemagglutination reaction in the diagnosis of the early phase of opisthorchiasis. I. A method of isolating erythrocyte samples and the results of their diagnostic use in experimental opisthorchiasis in golden hamsters, Med. Parazitol. (Mosk)., 50:20-23.
7. Klebanovskaia IA (1985) Indirect hemagglutination reaction in the diagnosis of the early phase of opisthorchiasis. 3. An economic method of obtaining a dried, erythrocyte opisthorchid antigenic diagnostic agent, Med. Parazitol. (Mosk)., 54:25-27.
8. Glupov VV, Khokhlova NI, Khvoshchevskaia MF, Vodianitskaia SN, Iurlova NI (1997) The use of immunoblotting for studying Opisthorchis felineus (Rivolta, 1884) antigens, Med. Parazitol. (Mosk)., 66:17-19.
9. Kotelkin AT, Razumov IA, Pokrovskii IV, Loktev VB (1997) A comparative study of the somatic, excretory-secretory and egg antigens of Opisthorchis felineus, Med. Parazitol. (Mosk)., 66:12-16.
10. Meniavtseva TA, Ratner GM, Struchkova SV, Kolmakova MV, Stepanova TF, Lepekhin AV, Maier VA, Postnikova TF (1996) Immunoenzyme analysis in the diagnosis of opisthorchiasis. I. The development of an immunoenzyme method for determining IgM antibodies to the Opisthorchis antigen, Med. Parazitol. (Mosk)., 65:41-43.
11. Wongratanacheewin S, Sirisinha S (1987) Analysis of Opisthorchis viverrini antigens: physicochemical characterization and antigen localization, Southeast Asian J. Trop. Med. Publ. Health., 18:511-520.
12. Wongratanacheewin S, Chawengkirttikul R, Bunnag D, Sirisinha S (1988) Analysis of Opisthorchis viverrini antigens by immunoprecipitation and polyacrylamide gel electrophoresis, Parasitology, 96:119-128.
13. Borovikov SN, Kuibagarov MA, Suranshiev ZhA, Baesheva DA, Atygaeva SK, Khalikova AS (2010) Preparation and study immunochemical properties of the opistorchis antigens [Poluchenie i izuchenie immunokhimicheskikh svoistv antigenov opistorkhov], Biothechnology. Theory and practice, 4:70-74. (In Russian)
14. Lvova MN, Tangkawattana S, Balthaisong S, Katokhin AV, Mordvinov VA, Spira B (2012) Comparative histopathology of Opisthorchis felineus and Opisthorchis viverrini in a hamster model: an implication of high pathogenicity of the European liver fluke, Parasitol Int., 61:167-172. DOI: 10.1016/j.parint.2011.08.005.
15. Fridlianskaia II (1988) Preparation the polyclonal antibody. Cell culture techniques [Poluchenie poliklonal'nykh antitel. Metody kul'tivirovaniia kletok], Bullet of scientific papers, Nauka, Leningrad, 194-205. (In Russian)
16. Kiyan VS, Tokpan SS, Baibolin ZhK, Teterin AV, Mertasov AG (2015) Hematologic indicators of the dogs under artificial infection by opisthorchiasis [Gematologicheskie pokazateli sobak pri iskusstvennom zarazhenii opistorkhozom], Bulletin of PSU. A series of chemical and biological, Pavlodar, 3:12-20. (In Russian)
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Kiyan, V. S. (2016). Biochemical and immunological properties of excretory-secretory antigen Opisthorchis felineus. Experimental Biology, 67(2), 188–195. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/1194
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IMMUNOLOGY
