Transient expression of reporter GFP gene in tissues of Kazakh cotton varieties.
Keywords:
cotton, leaves, cotyledons, GFP-gene, transient expression.Abstract
Transient expression of GFP gene in cotton tissues (cotyledons and leaves) were shown. The micrographs showing the transient expression of GFP expression were obtained using the confocal microscope. The green-fluorescent protein (GFP) of the jellyfish Aequoreavictoria has recently been used as a universal reporter in a broad range of heterologous living cells and organisms and GFP is a simple and economical new tool for the direct visualization of promoter activities with a broad range of strength and cell specificity. It can be used to measure dynamic responses of signal transduction pathways, transfection efficiency, and subcellular localization of chimeric proteins, and should be suitable for many other applications in genetically modified living cells and tissues of higher plants.References
Литература/References
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1.D.C Prasher, V.K Eckenrode, W.W Ward, F.G Prendergast, M.J Cormier.Primary structure of the Aequoravictoria green-fluorescent protein //Gene., 1992, –P. 229–233.
2.A.B Cubitt, R Heim, SR Adams, AE Boyd, LA Gross, RY Understanding, improving and using green fluorescent proteins// Trends Biochem Sci.,1995.– Vol. 20. – P. 448-455.
3.R. Heim, D.C Prasher, R.Y Tsien. Wavelength mutations and posttranslational autoxidation of green fluorescent protein// ProcNatlAcad Sci., 1994. – P. 12501–12504.
4. S. Wang, T.Hazelrigg.Implications for bcd mRAN localization from spatial distribution of exu protein in Drosophila oogenesis// Nature., 1994.– P. 400-403.
5.R.Rizzuto, M.Brini, P.Pizzo, M.Murgia, T.Pozzan. Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells// Curr Biol., 1995.– Vol. 15. – P. 635-642.
6. Hellens R., Mullineaux P., Klee H. A guide to Agrobacterium binary Ti vectors // Trends Plant Sci. 2000. V. 5. P. 446–451.
7. Chakrabarty R., Banerjee R., Chung S.M. et al. PSITE vectors for stable integration or transient expression of autofluorescent protein fusions in plants: probing Nicotianabenthamianavirus interactions // Mol. PlantMicrobe Interact. 2007. V. 20. № 7. P. 740–750.
How to Cite
Yertayeva, B. E., & Amitava Mitra, A. (2016). Transient expression of reporter GFP gene in tissues of Kazakh cotton varieties. Experimental Biology, 63(1), 60–63. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/1003
Issue
Section
Experimental biology. Biological bases of genofonds and the development of scientific research in the field of plant
