КЛОНИРОВАНИЕ И ЭКСПРЕССИЯ кДНК ЭНДО-Β-1,4-ГЛЮКАНАЗЫ ГРИБА ASPERGILLUS NIGER В E. coli И ХАРАКТЕРИСТИКА РЕКОМБИНАНТНОГО БЕЛКА

Abstract

EG enzymes are key components in fungal cellulase systems, and their functional activity is critical for hydrolysis of crystalline cellulose. The EG cDNA from A.niger was cloned into E. coli. The method of cloning consisted gene amplification with specific primers. The product of PCR was cloned into E. coli vector plasmid under control of T7 promoter. We showed expression of eng1 gene in E. coli recombinant strain and examined some properties of the recombinant protein. It showed maximal activity at 500C and pH 6.0