Optimization of the sterilization conditions of tau-sagyz explants (Scorzonera tau-saghyz Lipsch. et Bosse)

Abstract

In the article have been discussed about optimization of sterilization leaf and root explants of tau-sagyz. It was observed that effective agents for sterilization are 0.1 % solution of hydrargium. The apply 0.1% hydrargium solution’s and 70% ethanol was optimal for sterilization of tau-sagyz explants. For introduction tau-sagyz plants in vitro culture were used Murashige-Skug (MS) and Gamborg-Evelegh B5 (B5) medium with different concentrations of phytohormones 2.4-D, kinetin, BAP and IAA.