Sheep pox viral envelope protein L1RΔ synthesis in plants

Abstract

Sheep pox viral gene SPPV-NISKHI-56 encodes ortholog of immunodominant protein L1R of vaccinia virus. Gene sequence that encodes hydrophilic part of the protein (L1RΔ) was artificially synthesized and cloned in pET-19b plasmid for expression in bacteria. Recombinant DNA – construct based on pCAMBIA2300 vector was designed for transformation of the tobacco plants (Nicotiana tabacum L. cv. Samsun-NN). This construct contained 35S promoter of cauliflower mosaic virus, 5´UTR of gRNA AMV, His-Tag, gene for L1RΔ protein and NOS terminator. Transformed regenerated plants that produced root on the medium with kanamycin were analyzed for the presence of necessary mRNA by RT-PCR. Recombinant protein was detected in several transgenic plants by specific antibodies for L1RΔ.