ИДЕНТИФИКАЦИЯ ТЕРМОФИЛЬНЫХ БАКТЕРИЙ ИЗ ГОРЯЧЕГО ИСТОЧНИКА КАЗАХСТАНА И ОЦЕНКА ИХ ФЕРМЕНТАТИВНОЙ АКТИВНОСТИ
DOI:
https://doi.org/10.26577/eb.2023.v95.i2.06Ключевые слова:
термофильные бактерии, идентификация, 16S рРНК, филогенетический анализ, гидролитические ферменты.Аннотация
Термофильные микроорганизмы являются менее изученной, но важной группой микроорганизмов за счёт их способности продуцировать уникальные по своим свойствам ферменты. В связи с этим требуется изучение ранее не исследованных термальных источников на территории Казахстана для поиска новых теплолюбивых микроорганизмов с биотехнологическим потенциалом. Для этого в данной работе впервые были выделены и охарактеризованы термофильные бактерии из геотермального источника, Алматинская область, Республика Казахстан на предмет их способности продуцировать внеклеточные гидролитические ферменты (амилаза, целлюлаза, протеаза, и липаза). По результатам проведенных исследований было определено, что три из пяти изолятов показали высокую ферментативную активность на все четыре типа фермента и остальные продуцировали, по крайней мере, несколько внеклеточных гидролитических фермента. Филогенетический анализ по универсальному бактериальному гену 16s рРНК и результаты биохимической характеристики (на полосках API 50CHE и API Zym) показали, что все выделенные изоляты из термального источника отличаются от ныне известных видов рода Geobacillus, что в свою очередь указывает на то, что данный геотермальный источник является богатым местом обитания новых неизученных видов микроорганизмов с уникальными ферментами, более того один из пяти изолятов показал самую высокую оптимальную температуру роста при 75°С среди представителей данного рода.
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