Effect of temperature on the biomass yield, the synthesis of lipids and fatty acid composition of some bacteria
Keywords:
polyunsaturated fatty acids, microorganisms, culture conditions, total lipids, bacteria,Abstract
The influence of temperature on the cultivation of biomass yield, total lipid content and fatty acid composition of bacteria Pseudomonas peli, Flavobacterium saliperosum, Bacillus pumilus was studied. It was shown that the temperature reduction during cultivation of the bacteria leads to a decrease in the yield of lipids, but increases the concentration of unsaturated FA at the studied isolates.References
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2 Работнова И.Л., Позмогова И.Н. Хемостатное культивирование и ингибирование роста микроорганизмов. -М.: Наука, 1979. – 207 с.
3 Ratledge C. Fatty acid biosynthesis in microorganisms being used for Single Cell Oil production // Biochimie. –2004. – Vol. 8. – P. 807–815.
4 Егоров Н.С. Практикум по микробиологии. – М.: Изд-во МГУ, 1976. - С. 56 – 124.
5 Практикум по микробиологии / Под. ред. А.Н. Нетрусова. - М.: Academia. 2005. – С. 448- 597.
6 Кейтс М. Техника липидологии. - М.: Мир, 1975. – 234 с.
7 Folch J. A simple metod for the isolation and purification of total lipids from animals tissues // J. Biol. Chem. –1957. - № 226. – P. 497-509.
8 Garbus J., Deluca H.F., Loomans M.E., Strong F.M. 1963. Rapid incorporation of phosphate into mitochondrial lipids // J.Biol.Chem. – 1963. - Vol. 238. – P. 59-63.
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10 Дедюхина Э.Г., Ерошин В.К. Незаменимые химические элементы в регуляции метаболизма микроорганизмов // Успехи микробиологии. – 1992. – Т. 25. - С. 126-142.
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12 Cullen J., Phittlips M. C. Shipley G. G. The Effects of Temperature on the Composition and Physical Properties of the Lipids of Pseudomonas fluorescens // Biochem. J. – 1971. - № 125. – P. 733-742.
13 Ho S.Y., Chen F. Lipid characterization of Mortierella alpina grown at different NaCl concentrations // J. Agric. Food Chem. - 2008. – Vol. 56. № 17. - P. 7903–7909.
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