Оптимизация условий агробактериальной трансформации пыльцы хлопчатника казахстанской селекции
Аннотация
Оптимизирован способ получения трансгенных растений отечественного хлопчатника при помощи метода агробактериальной трансформации пыльцы. Получены трансгенные растения хлопчатника с. Мактаарал 4005 с встроенными репортерным GUS- геном и селективным маркерным nptII- геном.
Библиографические ссылки
1 Ozyigit I.I., Kahraman M.V., Ercan O. Relation between Explant Age, Total Phenols and Regeneration Response in Tissue Cultured Cotton (Gossypium hirsutum L.)// African Journal. Biotechnolnology., 2007. – V.6. – P.3-8.
2 Умбетаев И.И. Технология возделывания отечественных сортов хлопчатника – Алматы. – 2005. – 235 с
3 Umbeck p., Johnson G., Barton K., Swain W. Genetically transformed cotton (Gossypium hirsutum L.) plants // Biotechnology, 1987. – V. 5. – P. 263-265.
4 Umbeck P., Swain W., Yang N.S. Inheritance and eхpression of genes for kanamycin and chloramphenicol resistance in transgenic cotton plants // Crop Science, 1989. – V. 29. – P. 196-201.
5 Firoozabady E., De Boer D.L., Merlo D.J., Halk E.L., Amerson L.N., Rashka K.E., Murray E.F. Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants // Plant Molecular Biology, 1987. – V. 10. – P. 105-116.
6 Cousins Y.L., Lyon B.R., Llewellyn D.J. Transformation of an Australian cotton cultivar: Prospects for cotton improvement through genetic engineering // Australian Journal Plant Physiology, 1991. – V. 18. – P. 481-494.
7 Mishra R., Wang H., Yadav N.R., Wilkins T. Development of a highly regenerable elite Acala cotton (Gossypium hirsutum cv. Maxxa) - a step towards genotype independent regeneration // Plant Cell Tissue Org. Cult., 2003. – V. 73. – P. 21-35.
8 Zapata C., Park S.H., El-Zik K.M., Smith R.H. Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex // Theoretical and Applied Genetics, 1999. – V. 98. – P. 252-256.
9 Li X., Wang X.D., Zhao X., Dutt Y. Improvement of cotton fiber quality by transforming the acsA and acsB genes into Gossypium hirsutum L. by means of vacuum infiltration //Plant Cell Report, 2004. – V. 22. – P. 691-697.
10 Chaudhary B., Kumar S., Prasad K.V.S., Oinam G.S., Burma P.K., Pental.D. Slow desiccation leads to high- frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L cv.Coker 310 FR). // Plant Cell Rep., 2003. – V. 21. – P. 955-960.
11 Jefferson R.A. Assaying chimeric genes in plants: the GUS gene fusion system // Plant Mol. Biol. Rep., 1987. – V.5. – P. 387-405
12 Chaudhary B., Kumar S., Prasad K.V.S., Oinam G.S., Burma P.K., Pental.D. Slow desiccation leads to high- frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L cv.Coker 310 FR). // Plant Cell Rep., 2003. – V. 21. – P. 955-960.
13 Маниатис Т., Фрич Э., Сэмбрук Дж. Методы генетической инженерии. – Москва «Мир», 1984.
2 Умбетаев И.И. Технология возделывания отечественных сортов хлопчатника – Алматы. – 2005. – 235 с
3 Umbeck p., Johnson G., Barton K., Swain W. Genetically transformed cotton (Gossypium hirsutum L.) plants // Biotechnology, 1987. – V. 5. – P. 263-265.
4 Umbeck P., Swain W., Yang N.S. Inheritance and eхpression of genes for kanamycin and chloramphenicol resistance in transgenic cotton plants // Crop Science, 1989. – V. 29. – P. 196-201.
5 Firoozabady E., De Boer D.L., Merlo D.J., Halk E.L., Amerson L.N., Rashka K.E., Murray E.F. Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants // Plant Molecular Biology, 1987. – V. 10. – P. 105-116.
6 Cousins Y.L., Lyon B.R., Llewellyn D.J. Transformation of an Australian cotton cultivar: Prospects for cotton improvement through genetic engineering // Australian Journal Plant Physiology, 1991. – V. 18. – P. 481-494.
7 Mishra R., Wang H., Yadav N.R., Wilkins T. Development of a highly regenerable elite Acala cotton (Gossypium hirsutum cv. Maxxa) - a step towards genotype independent regeneration // Plant Cell Tissue Org. Cult., 2003. – V. 73. – P. 21-35.
8 Zapata C., Park S.H., El-Zik K.M., Smith R.H. Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex // Theoretical and Applied Genetics, 1999. – V. 98. – P. 252-256.
9 Li X., Wang X.D., Zhao X., Dutt Y. Improvement of cotton fiber quality by transforming the acsA and acsB genes into Gossypium hirsutum L. by means of vacuum infiltration //Plant Cell Report, 2004. – V. 22. – P. 691-697.
10 Chaudhary B., Kumar S., Prasad K.V.S., Oinam G.S., Burma P.K., Pental.D. Slow desiccation leads to high- frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L cv.Coker 310 FR). // Plant Cell Rep., 2003. – V. 21. – P. 955-960.
11 Jefferson R.A. Assaying chimeric genes in plants: the GUS gene fusion system // Plant Mol. Biol. Rep., 1987. – V.5. – P. 387-405
12 Chaudhary B., Kumar S., Prasad K.V.S., Oinam G.S., Burma P.K., Pental.D. Slow desiccation leads to high- frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L cv.Coker 310 FR). // Plant Cell Rep., 2003. – V. 21. – P. 955-960.
13 Маниатис Т., Фрич Э., Сэмбрук Дж. Методы генетической инженерии. – Москва «Мир», 1984.
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Опубликован
2010-02-09
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БИОТЕХНОЛОГИЯ
