Оптимизация условий культивирования штаммов рода Streptomyces, составляющих биопрепарат для растениеводства
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Ключевые слова:
стрептомицет, антифунгальная активность, ростстимулирующее действие, зерновые культурыАннотация
Изучен рост биомассы штаммов Streptomyces candidus ИМВ 37 и Streptomyces canofumeus ИМВ 541, составляющих препарат для растениеводства, и биосинтез ими биологически активных веществ на 10 органических и синтетических средах. Показано значительное влияние состава питательных сред на рост штаммов стрептомицетов и уровень образования биологически активных веществ. Оптимальный рост биомассы штаммов стрептомицетов обеспечивали органические среды с соевой мукой. Среда № 8 с овсяной мукой является наиболее оптимальной для синтеза как антифунгального антибиотика, так и веществ, стимулирующих рост растений. Среда № 8 обеспечивает высокий уровень накопления биомассы (45,8 г/л) штамма Streptomyces canofumeus ИМВ 541, а также высокую антифунгальную активность культуральной жидкости (диаметр зоны подавления роста тест-организмов 31-32 мм) и экстрактов из биомассы (36 мм). Культуральная жидкость штамма Streptomyces candidus ИМВ 37, полученная на среде № 8, увеличивает энергию прорастания семян пшеницы на 26,6%, лабораторную всхожесть на 20,0 %, длину проростков в 2,2 раза, длину корня в 2,0 раза, сырую массу растений в 1,9 раза.
Библиографические ссылки
Литературa
1 Joo G.J. Production of an antifungal substance for biological control of Phytophthora capsici causing phytophthora blight in red-peppers by Streptomyces halstedii // Biotechnol. Lett. – 2005. – Vol. 27. – P. 201–205.
2 Aryantha P.G., Guest I.D. Mycoparasitic and antagonistic inhibition on Phytophtora cinnamomi Rands by microbial agents isolated from manure composts // Plant Pathology. – 2006. – Vol. 5. – P. 291-298.
3 Propagdee B., Kuekulvong C., Mongkolsuk S. Antifungal potential of extracellular metabolites producer by Streptomyces hygroscopicus againts phytopathogenic fungi // Int. J. Biol. Sci. – 2008. – Vol. 4. – P. 330-337.
4 Дегтярева Е.А., Виноградова К.А., Александрова А.В., Филоненко В.А., Кожевин П.А. Почвенные актиномицеты как потенциальные биофунгициды // Вестник Московского университета, сер. почвоведение. - 2009. - № 2. - С. 22-26.
5 Snehal J., Shinde S.K., Prashanthi P.U., Krishnaraj J. Identification and utilization of actinobacteria for biocontrol of rice sheath blight pathogen Rhizoctonia solani // Asian Journal of Bio Science. – 2014. – Vol. 9, N 2. – P. 227-233.
6 Bouras N., Meklat A., Toumatia O., Mokrane S., Holtz M.D., Strelkov S.E., Sabaou N. Bioactive potential of a new strain of Streptomyces sp. PP14 isolated from Canadian soil // Afr. J. Microbiol. Res. – 2013. - Vol. 7. – N 25. – P. 3199-3208.
7 Широких И.Г. и др. Эффекты интродукции Streptomices hygroscopicus А4 в фитосферу голозерного овса // Зерновое хозяйство России. - 2013. - № 3. - С. 52-56.
8 Brown M.E. Plant growth substances produced by microorganisms of soil and rhizosphere // Journal of Applied Bacteriology. – 1972. – Vol. 35. – P. 443-451.
9 Martens D.A., Frankenberger W.T. Assimilation of exogenous 2'-14C-indole acetic acid and 3'-14C-tryptophan exposed to the roots of three wheat varieties // Plant and Soil. – 1994. – Vol. 166. – P. 281-290.
10 Tokala R.K., Strap J.L., Jung C.M., Crawford D.L., Salove M.H., Deobald L.A., Bailey J.F., Morra M.J. Novel Plant-Microbe Rhizosphere Interaction Involving Streptomyces lydicus WYEC108 nd the Pea Plant (Pisum sativum) // Applied and Environmental Microbiology. – 2002. – Vol. 68. – P. 2161-2171.
11 Waksman S.A. Classification, Identification and Description of Genera and Species. The Actinomycetes. - Baltimore: Waverly Press, 1961. – Vol. 2. – 962 p.
12 Ilić S., Konstantinović S., Veljković B., Savić S., Gojgić-Cvijović G. The impact of different carbon and nitrogen sources on antibiotic production by Streptomyces hygroscopicus CH-7 // Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. - 2010. – Vol. 2. – P. 1337-1342.
13 Reddy N.G., Ramakrishna D., Rajagopal S. Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites // Egyptian Journal of Biology. – 2011. – Vol. 13. – P. 21-29.
14 Егоров Н.С. Основы учения об антибиотиках. - М.: МГУ, Наука, 2004. - 528 с.
15 Locci R. Streptomyces and Related Genera. Bergey’s Manual of Systematic Bacteriology.
- Baltimore: Williams Co, 1989. - Vol. 4. – P. 2451–2508.
16 Salha H.M., Al-Zahrani. Studies on the Antimicrobial Activity of Streptomyces // J. KAU Sci. – 2007. - Vol. 19. – P. 127-138.
17 Gupte M.D., Kulkarni P.R. A study of antifungal antibiotic production by Streptomyces chattanougensis MTCC 3423 using full factorial design // Lett. Appld. Microbiol. – 2002. – Vol. 35, № 1. – P. 22-26.
18 Vieira M.F., Sousa Q., Lopes C.E., Pereira N. J. Production of Actinomycin D by Streptomyces parvulus // Braz. Arch. Biol. Technol. - 2001. – Vol. 44, № 3. – P. 14-22.
19 ГОСТ 12038-84. Семена сельскохозяйственных культур. Методы определения всхожести. Изменение №2 к ГОСТ 12038-84 от 01.06.1995.
20 Урбах В.Ю. Статистический анализ в биологических и медицинских исследованиях. - М.: Медицина, 1975. - 295 с.
References
1 Joo GJ (2005) Production of an antifungal substance for biological control of Phytophthora capsici causing phytophthora blight in red-peppers by Streptomyces halstedii. Biotechnol Lett, 27:201–205.
2 Aryantha PG, Guest ID (2006) Mycoparasitic and antagonistic inhibition on Phytophtora cinnamomi Rands by microbial agents isolated from manure composts. Plant Pathology, 5:291-298.
3 Propagdee B, Kuekulvong C, Mongkolsuk S (2008) Antifungal potential of extracellular metabolites producer by Streptomyces hygroscopicus againts phytopathogenic fungi. Int J Biol Sci, 4:330-337.
4 Degtjareva EA, Vinogradova KA, Aleksandrova AV, Filonenko VA, Kozhevin PA (2009) Soil actinomycetes as potential fungicides. News of Moscow University, ser. soil science [Pochvennye aktinomicety kak potencial'nye biofungicidy. Vestnik Moskovskogo universiteta, ser. pochvovedenie] 2:22-26. (In Russian)
5 Snehal J, Shinde SK, Prashanthi PU, Krishnaraj J (2014) Identification and utilization of actinobacteria for biocontrol of rice sheath blight pathogen Rhizoctonia solani. Asian Journal of Bio Science, 9:227-233.
6 Bouras N, Meklat A, Toumatia O, Mokrane S, Holtz MD, Strelkov SE, Sabaou N (2013) Bioactive potential of a new strain of Streptomyces sp. PP14 isolated from Canadian soil. Afr J Microbiol Res, 7:3199-3208.
7 Shirokih IG e a (2013) The effects of the introduction of Streptomyces hygroscopicus A4 in phytosphere naked oats. Grain farm in Russia [Jeffekty introdukcii Streptomices hygroscopicus A4 v fitosferu golozernogo ovsa. Zernovoe hozjajstvo Rossii] 3:52-56. (In Russian)
8 Brown ME (1972) Plant growth substances produced by microorganisms of soil and rhizosphere. Journal of Applied Bacteriology, 35:443-451.
9 Martens DA, Frankenberger WT (1994) Assimilation of exogenous 2'-14C-indole acetic acid and 3'-14C-tryptophan exposed to the roots of three wheat varieties. Plant and Soil, 166:281-290.
10 Tokala RK, Strap JL, Jung CM, Crawford DL, Salove MH, Deobald LA, Bailey JF, Morra MJ (2002) Novel Plant-Microbe Rhizosphere Interaction Involving Streptomyces lydicus WYEC108 nd the Pea Plant (Pisum sativum). Applied and Environmental Microbiology, 68:2161-2171.
11 Waksman SA (1961) Classification, Identification and Description of Genera and Species. The Actinomycetes. Baltimore, Waverly Press.
12 Ilić S, Konstantinović S, Veljković B, Savić S, Gojgić-Cvijović G (2010) The impact of different carbon and nitrogen sources on antibiotic production by Streptomyces hygroscopicus CH-7. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, 2:1337-1342.
13 Reddy NG, Ramakrishna D, Rajagopal S (2011) Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites. Egyptian Journal of Biology, 13:21-29.
14 Egorov NS (2004) Fundamentals of theory of antibiotics [Osnovy uchenija ob antibiotikah]. Moscow State University, Moscow, Russia (In Russian)
15 Locci R (1989) Streptomyces and Related Genera. Bergey’s Manual of Systematic Bacteriology. Baltimore: Williams Co, 4:2451–2508.
16 Salha HM, Al-Zahrani (2007) Studies on the Antimicrobial Activity of Streptomyces. J KAU Sci, 19:127-138.
17 Gupte MD, Kulkarni PR (2002) A study of antifungal antibiotic production by Streptomyces chattanougensis MTCC 3423 using full factorial design. Lett Appld Microbiol, 35:22-26.
18 Vieira MF, Sousa Q, Lopes CE, Pereira NJ (2001) Production of Actinomycin D by Streptomyces parvulus. Braz Arch Biol Technol, 44:14-22.
19 GOST 12038-84. Agricultural seeds. Methods for determining the germination. Change №2 to GOST 12038-84 from 01.06.1995 [GOST 12038-84. Semena sel'skohozjajstvennyh kul'tur. Metody opredelenija vshozhesti. Izmenenie #2 k GOST 12038-84 ot 01.06.1995] (In Russian)
20 Urbach VY (1975) Statistical analysis in biological and medical research [Statisticheskij analiz v biologicheskih i medicinskih issledovanijah]. Medicine, Moscow, Russia (In Russian)
1 Joo G.J. Production of an antifungal substance for biological control of Phytophthora capsici causing phytophthora blight in red-peppers by Streptomyces halstedii // Biotechnol. Lett. – 2005. – Vol. 27. – P. 201–205.
2 Aryantha P.G., Guest I.D. Mycoparasitic and antagonistic inhibition on Phytophtora cinnamomi Rands by microbial agents isolated from manure composts // Plant Pathology. – 2006. – Vol. 5. – P. 291-298.
3 Propagdee B., Kuekulvong C., Mongkolsuk S. Antifungal potential of extracellular metabolites producer by Streptomyces hygroscopicus againts phytopathogenic fungi // Int. J. Biol. Sci. – 2008. – Vol. 4. – P. 330-337.
4 Дегтярева Е.А., Виноградова К.А., Александрова А.В., Филоненко В.А., Кожевин П.А. Почвенные актиномицеты как потенциальные биофунгициды // Вестник Московского университета, сер. почвоведение. - 2009. - № 2. - С. 22-26.
5 Snehal J., Shinde S.K., Prashanthi P.U., Krishnaraj J. Identification and utilization of actinobacteria for biocontrol of rice sheath blight pathogen Rhizoctonia solani // Asian Journal of Bio Science. – 2014. – Vol. 9, N 2. – P. 227-233.
6 Bouras N., Meklat A., Toumatia O., Mokrane S., Holtz M.D., Strelkov S.E., Sabaou N. Bioactive potential of a new strain of Streptomyces sp. PP14 isolated from Canadian soil // Afr. J. Microbiol. Res. – 2013. - Vol. 7. – N 25. – P. 3199-3208.
7 Широких И.Г. и др. Эффекты интродукции Streptomices hygroscopicus А4 в фитосферу голозерного овса // Зерновое хозяйство России. - 2013. - № 3. - С. 52-56.
8 Brown M.E. Plant growth substances produced by microorganisms of soil and rhizosphere // Journal of Applied Bacteriology. – 1972. – Vol. 35. – P. 443-451.
9 Martens D.A., Frankenberger W.T. Assimilation of exogenous 2'-14C-indole acetic acid and 3'-14C-tryptophan exposed to the roots of three wheat varieties // Plant and Soil. – 1994. – Vol. 166. – P. 281-290.
10 Tokala R.K., Strap J.L., Jung C.M., Crawford D.L., Salove M.H., Deobald L.A., Bailey J.F., Morra M.J. Novel Plant-Microbe Rhizosphere Interaction Involving Streptomyces lydicus WYEC108 nd the Pea Plant (Pisum sativum) // Applied and Environmental Microbiology. – 2002. – Vol. 68. – P. 2161-2171.
11 Waksman S.A. Classification, Identification and Description of Genera and Species. The Actinomycetes. - Baltimore: Waverly Press, 1961. – Vol. 2. – 962 p.
12 Ilić S., Konstantinović S., Veljković B., Savić S., Gojgić-Cvijović G. The impact of different carbon and nitrogen sources on antibiotic production by Streptomyces hygroscopicus CH-7 // Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. - 2010. – Vol. 2. – P. 1337-1342.
13 Reddy N.G., Ramakrishna D., Rajagopal S. Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites // Egyptian Journal of Biology. – 2011. – Vol. 13. – P. 21-29.
14 Егоров Н.С. Основы учения об антибиотиках. - М.: МГУ, Наука, 2004. - 528 с.
15 Locci R. Streptomyces and Related Genera. Bergey’s Manual of Systematic Bacteriology.
- Baltimore: Williams Co, 1989. - Vol. 4. – P. 2451–2508.
16 Salha H.M., Al-Zahrani. Studies on the Antimicrobial Activity of Streptomyces // J. KAU Sci. – 2007. - Vol. 19. – P. 127-138.
17 Gupte M.D., Kulkarni P.R. A study of antifungal antibiotic production by Streptomyces chattanougensis MTCC 3423 using full factorial design // Lett. Appld. Microbiol. – 2002. – Vol. 35, № 1. – P. 22-26.
18 Vieira M.F., Sousa Q., Lopes C.E., Pereira N. J. Production of Actinomycin D by Streptomyces parvulus // Braz. Arch. Biol. Technol. - 2001. – Vol. 44, № 3. – P. 14-22.
19 ГОСТ 12038-84. Семена сельскохозяйственных культур. Методы определения всхожести. Изменение №2 к ГОСТ 12038-84 от 01.06.1995.
20 Урбах В.Ю. Статистический анализ в биологических и медицинских исследованиях. - М.: Медицина, 1975. - 295 с.
References
1 Joo GJ (2005) Production of an antifungal substance for biological control of Phytophthora capsici causing phytophthora blight in red-peppers by Streptomyces halstedii. Biotechnol Lett, 27:201–205.
2 Aryantha PG, Guest ID (2006) Mycoparasitic and antagonistic inhibition on Phytophtora cinnamomi Rands by microbial agents isolated from manure composts. Plant Pathology, 5:291-298.
3 Propagdee B, Kuekulvong C, Mongkolsuk S (2008) Antifungal potential of extracellular metabolites producer by Streptomyces hygroscopicus againts phytopathogenic fungi. Int J Biol Sci, 4:330-337.
4 Degtjareva EA, Vinogradova KA, Aleksandrova AV, Filonenko VA, Kozhevin PA (2009) Soil actinomycetes as potential fungicides. News of Moscow University, ser. soil science [Pochvennye aktinomicety kak potencial'nye biofungicidy. Vestnik Moskovskogo universiteta, ser. pochvovedenie] 2:22-26. (In Russian)
5 Snehal J, Shinde SK, Prashanthi PU, Krishnaraj J (2014) Identification and utilization of actinobacteria for biocontrol of rice sheath blight pathogen Rhizoctonia solani. Asian Journal of Bio Science, 9:227-233.
6 Bouras N, Meklat A, Toumatia O, Mokrane S, Holtz MD, Strelkov SE, Sabaou N (2013) Bioactive potential of a new strain of Streptomyces sp. PP14 isolated from Canadian soil. Afr J Microbiol Res, 7:3199-3208.
7 Shirokih IG e a (2013) The effects of the introduction of Streptomyces hygroscopicus A4 in phytosphere naked oats. Grain farm in Russia [Jeffekty introdukcii Streptomices hygroscopicus A4 v fitosferu golozernogo ovsa. Zernovoe hozjajstvo Rossii] 3:52-56. (In Russian)
8 Brown ME (1972) Plant growth substances produced by microorganisms of soil and rhizosphere. Journal of Applied Bacteriology, 35:443-451.
9 Martens DA, Frankenberger WT (1994) Assimilation of exogenous 2'-14C-indole acetic acid and 3'-14C-tryptophan exposed to the roots of three wheat varieties. Plant and Soil, 166:281-290.
10 Tokala RK, Strap JL, Jung CM, Crawford DL, Salove MH, Deobald LA, Bailey JF, Morra MJ (2002) Novel Plant-Microbe Rhizosphere Interaction Involving Streptomyces lydicus WYEC108 nd the Pea Plant (Pisum sativum). Applied and Environmental Microbiology, 68:2161-2171.
11 Waksman SA (1961) Classification, Identification and Description of Genera and Species. The Actinomycetes. Baltimore, Waverly Press.
12 Ilić S, Konstantinović S, Veljković B, Savić S, Gojgić-Cvijović G (2010) The impact of different carbon and nitrogen sources on antibiotic production by Streptomyces hygroscopicus CH-7. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, 2:1337-1342.
13 Reddy NG, Ramakrishna D, Rajagopal S (2011) Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites. Egyptian Journal of Biology, 13:21-29.
14 Egorov NS (2004) Fundamentals of theory of antibiotics [Osnovy uchenija ob antibiotikah]. Moscow State University, Moscow, Russia (In Russian)
15 Locci R (1989) Streptomyces and Related Genera. Bergey’s Manual of Systematic Bacteriology. Baltimore: Williams Co, 4:2451–2508.
16 Salha HM, Al-Zahrani (2007) Studies on the Antimicrobial Activity of Streptomyces. J KAU Sci, 19:127-138.
17 Gupte MD, Kulkarni PR (2002) A study of antifungal antibiotic production by Streptomyces chattanougensis MTCC 3423 using full factorial design. Lett Appld Microbiol, 35:22-26.
18 Vieira MF, Sousa Q, Lopes CE, Pereira NJ (2001) Production of Actinomycin D by Streptomyces parvulus. Braz Arch Biol Technol, 44:14-22.
19 GOST 12038-84. Agricultural seeds. Methods for determining the germination. Change №2 to GOST 12038-84 from 01.06.1995 [GOST 12038-84. Semena sel'skohozjajstvennyh kul'tur. Metody opredelenija vshozhesti. Izmenenie #2 k GOST 12038-84 ot 01.06.1995] (In Russian)
20 Urbach VY (1975) Statistical analysis in biological and medical research [Statisticheskij analiz v biologicheskih i medicinskih issledovanijah]. Medicine, Moscow, Russia (In Russian)
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Trenozhnikova, L. P., Ultanbekova, G. D., Balgimbaeva, A. S., Galimbaeva, R. S., & Masirbaeva, A. (2016). Оптимизация условий культивирования штаммов рода Streptomyces, составляющих биопрепарат для растениеводства. Вестник КазНУ. Серия биологическая, 67(2), 168–175. извлечено от https://bb.kaznu.kz/index.php/biology/article/view/1192
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