Optimization of Agrobacterium– mediated transformation conditions for cotton
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Keywords:
the cotton, the Agrobacterium-mediated transformation,Abstract
In this article are provided a data on optimization of Agrobacterium–mediated transformation conditions for cotton domestic varieties. The optimal concentration of acetosyringone (100 μM), the optical density of a suspension of bacterial cells (0,6) and co-cultivations time (48 hours) with A.tumefaciens strain LBA4404 for the Agrobacteriummediated transformation of cotton explants are revealed. The results will be used for creation of transgenic plants ofdomestic cotton varieties resistant to herbicide Basta.References
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2 Herouet C., Esdaile D.J., Mallyon B.A. et al. Safety evaluation of the phosphinothricinacetyltransferase proteins encoded by the pat and bar sequences that confer tolerance to glufosinate-ammonium herbicide in transgenic plants // Regulatory Toxicology and Pharmacology. – 2005. – Vol. 41. – P. 134–149.
3 Brimner T.A., Gallivan G.J., Stephenson G.R. Influence of herbicide-resistant canola on the environmental impact of weed management // Pest Management Science. – 2005. – Vol. 61. – P. 47-52.
4 Murashige T., Skoog F. A revised medium for rapid growth and biosynthesis with tobacco tissue culture //Plant Physiology.- 1962. – Vol. 15. – P. 473-497.
5 Jefferson R. A., Kavanagh T.A., Bevan M. W. GUS fusions: β-B-glucuronidase as a sensitive and versatile gene fusion marker in higher plants // The EMBO Journal. – 1987. – Vol. 6. – P.3901 -3907.
6 Sunilkumar G., Rathore K. S. Transgenic cotton: factors influencing Agrobacterium-mediated transformation and regeneration // Molecular Breeding. – 2001. – Vol. 8. –P. 37–52.
7 Zapata C., Park S.H., El-Zik K.M. and Smith R.H. Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex //Theoretical and Applied Genetics. – 1999.- Vol. 98.– P. 252–256.
8 Khan T., Reddy V. S., Leelavathi S. High-frequency regeneration via somatic embryogenesis of an elite recalcitrant cotton genotype (GossypiumhirsutumL.) and efficient Agrobacterium-mediated transformation // Plant Cell Tissue and Organ Culture. – 2010. –Vol. 101. – P. 323-330.
9 Khan T., Reddy V. S., Leelavathi S. High-frequency regeneration via somatic embryogenesis of an elite recalcitrant cotton genotype (Gossypium hirsutum L.) and efficient Agrobacterium-mediated transformation // Plant Cell Tissue and Organ Culture. – 2010. –Vol. 101. – P. 323-330.
10 Rashid H., Yokoi S., Toriyama K. and Hinata K. Trans-genic plant production mediated by Agrobacterium in Indicarice //Plant Cell Reports. – 1996. – Vol. 15. – P. 727–730.
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Manabayeva., S. A., & Rahimzhanova, A. O. (2015). Optimization of Agrobacterium– mediated transformation conditions for cotton. Experimental Biology, 60(1), 200–203. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/94
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Bioengineering of Plants
