ТРАНСФОРМАЦИЯ КОНСТРУКЦИЙ, СОДЕРЖАЩИХ ГЕН β-ГЛЮКУРОНИДАЗЫ И РАЗЛИЧНЫЕ ЭНХАНСЕРНЫЕ ЭЛЕМЕНТЫ В Arabidopsis thaliana
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Keywords:
Floral Dip, DNA,Abstract
As the result of the experiment the translational enhancing elements containing DNA constructs were created and were transformed into Arabidopsis thaliana by “Floral Dip” protocol.References
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2.Gallie, D.R. Translational control of cellular and viral mRNAs//, Plant Mol. Biol. 1996, V.32, P.145-158.
3.Fuetterer, J. and Hohn, T. Translation in plants-rules and exceptions.// Plant Mol. Biol., 1996, V. 32, P.159-189.
4.Акбергенов Р.Ж. Исследование молекулярных механизмов инициации трансляции мРНК у растений(автореферат). – А: Домино, 2002, 26с.
5.R. Zh. Akbergenov, S. Sh. Zhanybekova, R. V. Kryldakov, A. Zhigailov, N. S. Polimbetova, T. Hohn and B. K. Iskakov. ARC-1, a sequence element complementary to an internal 18S rRNA segment, enhances translation efficiency in plants when present in the leader or intercistronic region of mRNAs // Nucleic Acids Research, 2004, Vol. 32, N. 1, P. 239-247.
6.Gallie, D.R., Sleat, D.E., Watts, J.W., Turner, P.C. and Wilson, T. M. A. The 5’-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo// Nucleic Acids Res. 1987, V. 15, P. 3257-3273.
7.Maniatis T. et al. Molecular cloning, book I, 1989.
8.Bent A. Arabidopsis thaliana floral dip transformation method // Methods Mol Biol. 2006, V. 343, P. 87-103.
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Ережепов, Д. А., Богуспаев, К. К., Искаков, Б. К., & Хеберле-Борс, Э. Э. (2009). ТРАНСФОРМАЦИЯ КОНСТРУКЦИЙ, СОДЕРЖАЩИХ ГЕН β-ГЛЮКУРОНИДАЗЫ И РАЗЛИЧНЫЕ ЭНХАНСЕРНЫЕ ЭЛЕМЕНТЫ В Arabidopsis thaliana. Experimental Biology, 40(1), 85–88. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/590
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BIOTECHNOLOGY, BIOCHEMISTRY AND PLANTS PHYSIOLOGY
