ҚОЖАҚАТ МИКРОӨСІМДІКТЕРІНДЕГІ IN VITRO ЖАҒДАЙЫНДА ҚАРҚЫНДЫ ӨСУ ЖƏНЕ ҚАРТАЮ ПРОЦЕСТЕРІНЕ ƏСЕР ЕТУШІ КЕЙБІР ФИЗИОЛОГИЯЛЫҚ КӨРСЕТКІШТЕРДІҢ ДИНАМИКАСЫ
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Keywords:
biochemical, analys,Abstract
Some biochemical parameters of blackberry microplants stored under long-term in vitro preservation at +5 °С have been analysed. It is established, that during the long-term in vitro preservation in conditions at +5 °С the content of an ascorbic acid in leaves of a blackberry is sharply reduced (in 25 times within 8 months of preservation). The content of free proline increases in the beginning of preservation, and then is reduced. Level of Н2О2 in leaves of the blackberry, stored long time at low temperature, does not vary within the first 4 months and then increases. Peroxidase activity increases in 4-8 months of preservation and is decreased by 12 month of preservation. It is concluded that the most probable reason of ageing and destruction of microplants in conditions of long-term in vitro preservation is ncrease of a Н2О2 level.References
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2. Дунаева С. Е., Гавриленко Т. А. Коллекции плодовых и ягодных культур in vitro: стратегия создания и хранение // Труды по прикладной ботанике, генетике и селекции. 2007. Т. 161. С. 10–19.
3. Foyer C. H., Noctor G. Oxidant and antioxidant signalling in plants: a re-evaluationof the concept of oxidative stress in a physiological context // Plant, Cell and Environment. – 2005. – V. 28. – P. 1056–1071.
4. Pignocchi C., Foyer C. H. Apoplastic ascorbate metabolism and its role in the regulation of cell signaling // Current Opinion in Plant Biology. – 2003. – V. 6. – P. 379–389.
5. Fujiyama K., Intapruk C., Shinmyo A. Gene structures of peroxidases isoenzymes in horseradish and Arabidopsis thaliana and their expression // Biochem. Soc. Transact. – 1995. – V. 23. – P. 245–246.
6. Шарова Е. И. Клеточная стенка растений. – СПб.: Изд-во С.-Петербургского университета, 2004. –156 с.
7. Göring H. Proline accumulation under conditions of stress and deficiency of mineral nutrients // Miner. Nutr. Plants Proc. 1st Int. Symp. Plant Nutr., Varna, 1979, Sofia, 1979. – V. 1. – P. 103–117.
8. Rui H. T., Heinke F., Goring H. Zur Prolineakkumulation bei stress und Nahrstoffmangel // Wiss. S. Pad. Hochach. “Liselotte –Herrman” Gustrow. Math.-naturwiss. Fak. – 1980. – V. 18, N 1. – P. 162–166.
9. Roe J. H., Khuether C. A. Methods biochem. Anal. // J. Biol. Chem. 1948. Vol. 174. P. 201.
10. Bates S. L., Wilderen R. P., Teany I. D. Rapid determination of free proline for water-stress studies // Plant and Soil. 1973. Vol. 39. P. 205–207.
11. Jiang Z. Y., Woollard A. C. S., Wolff S. P. Hydrogen peroxide production during experimental protein glycation // FEBS. 1990. Vol. 268, N1. P. 69–71. Jiang Z. Y., Woollard A. C. S., Wolff S. P. Hydrogen peroxide production during experimental protein glycation // FEBS. 1990. Vol. 268, N1. P. 69–71.
12. Gay C., Gebicki J. M. A critical evaluation of the effect of sorbitol on the ferric-xylenol orange hydroperoxide assay // Anal. Biochem. 2000. Vol. 284. P. 217–220.
13. Lin C. C., Kao C. H. NaCl induced changes in ionically bound peroxidase activity in roots of rice seedlings // Pland and Soil. 1999. Vol. 216. P. 147–153.
14. Рокицкий П. Ф. Биологическая статистика. – Минск: Выс.шк., 1987. – 327с.
15. Chen Z., Yong T. E., Ling J., Chang S-C., Galie D. R. Increasing vitamin C content of plants through
anhanced ascorbate recycling // Proc. Natl. Acad. Sci. USA. – 2003. – V. 100. P. 3525–3530.
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Саматова, И. С. (2009). ҚОЖАҚАТ МИКРОӨСІМДІКТЕРІНДЕГІ IN VITRO ЖАҒДАЙЫНДА ҚАРҚЫНДЫ ӨСУ ЖƏНЕ ҚАРТАЮ ПРОЦЕСТЕРІНЕ ƏСЕР ЕТУШІ КЕЙБІР ФИЗИОЛОГИЯЛЫҚ КӨРСЕТКІШТЕРДІҢ ДИНАМИКАСЫ. Experimental Biology, 47(1), 33–38. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/327
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BIOTECHNOLOGY, BIOCHEMISTRY AND PLANTS PHYSIOLOGY
