IN VITRO PROPAGATION FROM CULTURED APEX OF MODERN SHRUB ROSE CV. ALASKA
Keywords:
Alaska, several concentration,Abstract
A method for the microclonal propagation of modern shrub rose cv. ‘Alaska’ was developed. Apex explants from in vitro young shoots were excised and cultured on basal medium of Murashige and Skoog (1962, MS) containing several concentrations of BAP, NAA and kinetin. Multiple shoot formation of up to 3 shoots was obtained on MS medium supplemented with 0,5 mg/l BAP. Shoot readily rooted on ½ MS medium added with 0,5 mg/l IAA and 1,0 mg/l IBA.References
1. Elliot R..F. Axenic culture of meristem tips of Rosa multiflora // Planta, 1970, V.95. – P.183-186.
2. Jacob G., Allan P., Bornman C.N. Tissue culture studies on rose: use shoot tip explants // Agroplantae,1970, V.2. – P.25-28.
3. Rout G.R.., Mohapatra A.., Mohan J. S. Tissue culture of ornamental pot plant: A critical review on present scenario and future prospect // Biotechnology Advances, 2006, V.24. – P.531-560.
4. Drefahl A.., Quoirin M.G., Cuquel F.L. Micropropagation of Rosa × hybrida cv. Vegas via axillary buds // Acta Horticulturae, 2007, V. 751. – P. 407-411.
5. Previati A., Benelli C., Da Re F., Ozudogru A., Lambradi M. Micropropagation and in vitro conservation of
virus-free rose germplasm. Propagation of Ornamental Plants, 2008, V. 8. – P. 93-98.
6. Kanchanapoom K., Posayapisit N., Kanchanapoom K. In vitro flowering from cultured nodal explants of
rose (Rose hybrida L.) // Notulae Botanicae Horti Agrobotanici Cluj, 2009, V. 37. – P. 261-263.
7. Мухамбетжанов С.К., Нам С.В., Вечерко Н.А., Мурсалиева В.К. Факторы влияющие на рост и развитие роз in vitro // Биотехнология. Теория и практика. – 2010. - №1. – С. 41-52.
8. Сапаргали О., Нам С.В., Вечерко Н.А., Мухамбетжанов С.К., Мурсалиева В.К. Влияние генотипа на микроклональное размножение роз // Сб. тр. междунар. конф. «Актуальные проблемы ботанического
ресурсоведения», 12-13 мая, Алматы. – 2010. – С. 341-343.
Вестник КазНУ. Серия биологическая, №1 (47), 2011
33
9. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures // Physiologia Plantarum, 1962, V. 15. – P. 473-497.
10. Vu N.H., Anh P.H., Nhut, D.T. The role of sucrose and different cytokinins in the in vitro floral morphogenesis of rose (hybrid tea) cv. ‘First Prize’ // Plant Cell Tissue and Organ Culture, 2006, V. 87. – P. 315-320.
11. Wang G. Y, Yuan, M. F., Hong, Y. 2002. In vitro flower induction in roses. In Vitro Cellular and
Developmental Biology-Plant, 2002, V. 38. – P. 513-518.
12. Kumar A., Sood A., Plani U.T., Gupta A.K., Plani L.M.S. Micropropagation of Rosa damascene Mill. from mature bushes using thidiazuron // Journal of Horticultural Science and Biotechnology, 2001, V. 76. – P. 30-34.
13. Khosh-Khui M., Jabbarzadeh Z. Effects of several variables on in vitro culture of Damask Rose (Rosa damascena Mill.) // Acta Horticulturae, 2007, V. 751. – P. 389-393.
14. Douglas G.C., Rutledge C.B., Casey A.D., Richardson D.H.S. Micropropagation of floribunda ground cover and miniature roses // Plant Tissue Culture, 1989, V.19. – P. 55-64.
15. Ibrahim R., Debergh P.C. Factors controlling high efficiency of adventitious bud formation and plant regeneration from in vitro leaf explants of roses (Rosa hybrida) // Scientia Horticulturae, 2001, V. 88. – P. 41-57.
2. Jacob G., Allan P., Bornman C.N. Tissue culture studies on rose: use shoot tip explants // Agroplantae,1970, V.2. – P.25-28.
3. Rout G.R.., Mohapatra A.., Mohan J. S. Tissue culture of ornamental pot plant: A critical review on present scenario and future prospect // Biotechnology Advances, 2006, V.24. – P.531-560.
4. Drefahl A.., Quoirin M.G., Cuquel F.L. Micropropagation of Rosa × hybrida cv. Vegas via axillary buds // Acta Horticulturae, 2007, V. 751. – P. 407-411.
5. Previati A., Benelli C., Da Re F., Ozudogru A., Lambradi M. Micropropagation and in vitro conservation of
virus-free rose germplasm. Propagation of Ornamental Plants, 2008, V. 8. – P. 93-98.
6. Kanchanapoom K., Posayapisit N., Kanchanapoom K. In vitro flowering from cultured nodal explants of
rose (Rose hybrida L.) // Notulae Botanicae Horti Agrobotanici Cluj, 2009, V. 37. – P. 261-263.
7. Мухамбетжанов С.К., Нам С.В., Вечерко Н.А., Мурсалиева В.К. Факторы влияющие на рост и развитие роз in vitro // Биотехнология. Теория и практика. – 2010. - №1. – С. 41-52.
8. Сапаргали О., Нам С.В., Вечерко Н.А., Мухамбетжанов С.К., Мурсалиева В.К. Влияние генотипа на микроклональное размножение роз // Сб. тр. междунар. конф. «Актуальные проблемы ботанического
ресурсоведения», 12-13 мая, Алматы. – 2010. – С. 341-343.
Вестник КазНУ. Серия биологическая, №1 (47), 2011
33
9. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures // Physiologia Plantarum, 1962, V. 15. – P. 473-497.
10. Vu N.H., Anh P.H., Nhut, D.T. The role of sucrose and different cytokinins in the in vitro floral morphogenesis of rose (hybrid tea) cv. ‘First Prize’ // Plant Cell Tissue and Organ Culture, 2006, V. 87. – P. 315-320.
11. Wang G. Y, Yuan, M. F., Hong, Y. 2002. In vitro flower induction in roses. In Vitro Cellular and
Developmental Biology-Plant, 2002, V. 38. – P. 513-518.
12. Kumar A., Sood A., Plani U.T., Gupta A.K., Plani L.M.S. Micropropagation of Rosa damascene Mill. from mature bushes using thidiazuron // Journal of Horticultural Science and Biotechnology, 2001, V. 76. – P. 30-34.
13. Khosh-Khui M., Jabbarzadeh Z. Effects of several variables on in vitro culture of Damask Rose (Rosa damascena Mill.) // Acta Horticulturae, 2007, V. 751. – P. 389-393.
14. Douglas G.C., Rutledge C.B., Casey A.D., Richardson D.H.S. Micropropagation of floribunda ground cover and miniature roses // Plant Tissue Culture, 1989, V.19. – P. 55-64.
15. Ibrahim R., Debergh P.C. Factors controlling high efficiency of adventitious bud formation and plant regeneration from in vitro leaf explants of roses (Rosa hybrida) // Scientia Horticulturae, 2001, V. 88. – P. 41-57.
How to Cite
Nam, S. V., Vecherko, N. A., Mukhambetzhanov, S. K., & Mursalieva, V. K. (2009). IN VITRO PROPAGATION FROM CULTURED APEX OF MODERN SHRUB ROSE CV. ALASKA. Experimental Biology, 47(1), 30–32. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/324
Issue
Section
BIOTECHNOLOGY, BIOCHEMISTRY AND PLANTS PHYSIOLOGY
