Influenza virus circulation in swine populations in various regions of the Republic of Kazakhstan (2018 - 2019)

Authors

  • N. S. Ongarbayeva al-Farabi Kazakh National University, Kazakhstan, Almaty
  • N. G. Klivleyeva LLP Scientific Production Center for Microbiology and Virology, Almaty, Kazakhstan
  • N. T. Saktaganov LLP Scientific Production Center for Microbiology and Virology, Almaty, Kazakhstan
  • M. K. Kalkozhayeva LLP Scientific Production Center for Microbiology and Virology, Almaty, Kazakhstan
  • T. I. Glebova LLP Scientific Production Center for Microbiology and Virology, Almaty, Kazakhstan
  • G. V. Lukmanova LLP Scientific Production Center for Microbiology and Virology, Almaty, Kazakhstan
  • A. M. Baimukhametova LLP Scientific Production Center for Microbiology and Virology, Almaty, Kazakhstan
  • M. K. Mustafin A. Baytursynov Kostanay State University, Kostanay
  • Sh. G. Yerdenov Kostanay Research Veterinary Station, Kostanay
  • B. M. Mustafin Kostanay Research Veterinary Station, Kostanay
  • G. Kh. Оspanov Government agency "Abay District Territorial Inspection of the Committee of Veterinary Control and Supervision of the Ministry of Agriculture of the Republic of Kazakhstan"
  • R. J. Webby St Jude Children’s Research Hospital, Memphis, USA

DOI:

https://doi.org/10.26577/eb-2019-3-b7
        95 134

Abstract

In 2018 – 2019, 1104 nasopharyngeal swabs and 39 blood serums were collected from 2-6- month-old swine in peasant livestock farms located in seven regions of the Republic of Kazakhstan.

Virological examination of 1104 samples in the polymerase chain reaction has resulted in the discovery of the genetic material of influenza A viruses in 26 biological samples (2.36% of the total number of examined swine). When subtyping RNA, influenza A/H1N1 virus was identified in 1.45% of samples, A/H3N2 in 0.54% of cases, А/H1sw2009 in 0,36% of samples.

As a result of virological studies, three isolates from the North Kazakhstan oblast have been obtained from biological samples in chicken embryos that were identified as influenza A/H1N1 viruses in RT-PCR, HAI and NAI assays.

Serological studies of 39 blood serums in the hemagglutination   inhibition assay have established that one sample (2.56% of the total number of examined serums) was positive for influenza A swine/Iova/15/30 (HSW1N1) virus, the antibody titer was 1:40. In three animal serums (7.69%), antibodies in the titers of 1:20-1:40 were detected against influenza A/California/ 04/09 virus (A/H1N1pdm). Nine serums (23.08%) were positive for A/Panama/2007/99(H3N2) virus with anti-hemagglutinin titers of 1:20-1:40.

An enzyme immunoassay of 39 serums in six samples (15.38% of cases from the total number of examined samples) has revealed antibodies against A/H3N2 influenza virus.

The results obtained during screening of biosamples in the polymerase chain reaction, hemagglutination inhibition assay, and enzyme immunoassay indicate the co-circulation of influenza viruses A/H1N, A/H3N2 and А/H1sw2009 in swine populations in Kazakhstan and the need for further monitoring studies of pathogens in order to timely detect the etiological agent of animal epizootic in livestock farms.

 Key words: virus, influenza, antigen, serum, antibodies, monitoring, circulation

References

1. Brown I.H. (2000) The epidemiology and evolution of influenza viruses in pigs. Vet. Microbiol, vol. 74, рр. 29-46.
2. Deeva Je.G. (2008) Gripp. Na poroge pandemii [Influenza. On the verge of a pandemic]. – M.: GEOTAR-Media. pp. 16-44.
3. Douwdal W.A., Kendal A., Noble G.R. (1979) Influenza virus: Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infection. Washington, pp. 585-609.
4. Gendon Ju.Z. (2010) Svinoj gripp N1N1/Californija – strasti i fakty [H1N1 Swine Influenza / California - Passions and Facts]. Journal of Microbiology Epidemiology and Immunobiology, no 4, pp. 105-114.
5. Hause B. M., Oleson T. A., Bey R. F. et al. (2010) Antigenic categorization of contemporary H3N2 swine influenza virus isolates using a high-throughput serum neutralization assay. J. Vet. Invest, vol. 22, pp. 352-359.
6. Hoffmann E, Stech J, Guan Y et. al. (2001) Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol.- 146 (12), pp. 2275-89.
7. Ishmuhametova N.G. (2013) «Svinoi gripp» A(N1N1) i ego rasprostranenie sredi ljudej [«Swine influenza» A (H1N1) and its distribution among men]. Reports of National Academy of Sciences of the Republic of Kazakhstan, no 4, pp. 96-101.
8. Informacionnyj bjulleten' INFOSAN No. 2/2009 – “A/H1N1 Influenza: characteristics of the transmission from animal to human” http://www.who.int/foodsafety/fs_management/No_02_influenza_Apr09_ru_rev1.pdf.
9. Ikranbegijn R., Kuznetsova T.V., Grudinin M.P. et.al. (2012) Molekuljarno-geneticheskie svojstva pandemicheskogo virusa N1N1v, cirkulirovavshego na territorii Kazahstana (2009-2010) [Molecular genetic properties of pandemic H1N1v virus circulating in the territory of Kazakhstan (2009-2010)]. Vestnik NGU, vol. 10 (3), pp. 80-86.
10. Jong J.C., Paccaud M.H., Ronde-Veploop J.M et.al. (1988) “Isolation of swine – like influenza A(N1N1) viruses from man in Switzerland and Netherlands. Ann. Inst. Pasteur Virol, vol. 139, no 4, pp. 429-437.
11. Kaverin N.V., Smirnov Yu. A. (2003) Mezhvidovaya transmissiya virusov grippa A i problema pandemiy [An interspecies transmission of influenza A viruses and pandemics]. Problems of virology, no 3, pp. 4-10.
12. Kuznetsova T.V., Shamenova M.G., Baymakhanova B.B. et.al. (2009) Monitoring cirkuljacii virusa grippa A sredi svinej v Vostochnom Kazahstane [Monitoring of circulation of influenza A virus among pigs in East and Northern Kazakhstan]. Veterinarija, no5(9), pp. 52-54.
13. Kuznetsova T.V., Shamenova M.G., Glebova T.I. et al. (2014) Serodiagnostic testing of influenza virus in pigs in Northern Kazakhstan. Journal of Central Asian Нealth Service Research, no 1, pp. 22-26.
14. Klivleyeva N.G., Saktaganov N.T., Glebova T.I. et.al. (2017) Obnaruzhenie virusov grippa A(N1N1) u ljudej i svinej v regione severnogo Kazahstana v 2014-2016 gg. [Detection of influenza A(H1N1) viruses in humans and pigs in the northern Kazakhstan during 2014–2016]. News of the National Academy of Sciences of the Republic of Kazakhstan, no 5 (323), pp. 106-114.
15. Klivleyeva N.G., Glebova T.I., Baiseiit S.B. et.al. (2018) Vyjavlenie virusov grippa, cirkulirujushhih sredi svinej v razlichnyh regionah Kazahstana v vesennij period 2018 g. [Detection of influenza viruses circulating among swine in different regions of Kazakhstan during 2018 spring season]. Mikrobiologija zhәne virusologija, no 3(22), pp. 89-96.
16. Klivleyeva N.G., Glebova T.I., Kuznetsova T.V. et al. (2014) Virological monitoring of influenza virus circulation in swine population in the Republic of Kazakhstan. World Congress on Controversies, Debates & Consensus in Veterinary Medicine, Prague, pp. 79-80.
17. Kukushkin S.A., Baibikov T.Z., Kan'shina A.V., Chelysheva M.V. (2009) Gripp svinej (epizootologiya, diagnostika, mery bor'by i profilaktiki) [Swine influenza (epizootology, diagnosis, control and prevention measures)]. Veterinariya, no 9, pp. 3-7.
18. Laptev S.V., Jamnikova S.S., Sayatov M.Kh. et.al. (1987) Izuchenie biologicheskih i antigennyh svojstv virusov grippa A (N1N1), vydelennyh ot svinej v Vostochnom Kazahstane [Study on biological and antigenic properties of influenza A (H1N1) viruses isolated from swine in the East Kazakhstan]. Izvestija AN KazSSR, no 2, pp. 55-58.
19. Lvov D.K. (1987) Influenza A viruses a sum of populations with a common protected gene pool. Sov. Med. Rev. Virol., vol. 2, pp. 15-37.
20. Leuwerke B., Kitikoon P., Evans R., Thacker E. (2008) Comparison of three serological assays to determine the cross-reactivity of antibodies from eight genetically diverse U.S. Swine influenza viruses. J. Vet. Diagn. Invest., vol. 20. pp. 426-432.
21. Lukmanova G.V., Klivleyeva N.G., Shamenova M.G. et al. (2016) Serological analysis of circulation of influenza viruses among swines in Kazakhstan in 2013-2014. Veterinariya, no 1 (45) pp. 61-63.
22. Lukmanova G.V., Klivleyeva N.G., Shamenova M.G. et al. (2015) Serological analysis of circulation of influenza viruses А among swines in Kazakhstan in 2013-2014. International scientific journal, no 6, pp. 9.
23. Lager K. (2010) Aktual'nye virusnye bolezni svinej. Materialy seminara «Problemy infekcionnoj patologii svinej»: XVIII Moskovskij Mezhdunar. Veterinarnyj Kongress.- Moskva, рр. 12-18.
24. Mejer, D. (2007) Veterinarnaja laboratornaja medicina. Interpretacija i diagnostika [Veterinary laboratory medicine. Interpretation and diagnosis] / D. Mejer, Dzh. Harvi // Perevod s angl. – M.: Sofion, p. 456.
25. (2009) Metodicheskie ukazanija po obnaruzheniju virusa grippa svinej tipa A metodom polimeraznoj cepnoj reakcii v rezhime real'nogo vremeni. Vladimir: LLC «Tranzit-IKS»
26. Neumann N., Noda T., Kawaoka Y. (2009) Emergence and pandemic potential of swine-origin H1N1 influenza virus. Nature, vol. 459, pp. 931–939.
27. Ongarbayeva N.S., Saktaganov N.T., Kalkozhayeva M.K et.al. (2018) Cirkuljacija virusa grippa A/H1N1 sredi ljudej i svinej v severnom i zapadnom Kazahstane v 2017-2018 gg. [Circulation of A/H1N1 influenza virus among humans and swine in northern and western Kazakhstan during 2017-2018]. The Central Asian Scientific-practical Journal on Public Health, no 2 (59), pp. 112-115.
28. Reed L.J., Muench H.A. (1938) A simple method of estimation fifty percent andpoints. American Journal of tropical medicine and Hygiene, vol. 27, pp. 493-497.
29. Solov'ev B.V. (2006) Gripp: sovremennye predstavlenija. Vet. zhizn'. No 7-8 (April), pp. 14-15.
30. Swine influenza in pigs: Facts http://www.eurolab.ua/flu/2126/15843/. 03.06.2019
31. Tran G.M.K., Gerbaud L., Caprani A.C. 66. Scorpion model of Influenza A(H1N1). ISHEID Conf 2010, Toulon France.Poster P168, Internet.
32. Webster R.G., Bean W.J., Gorman O.T. et al. (1992) Evolution of ecology of influenza A viruses. Microbiol. Rev., vol. 56, pp. 152-179.

Downloads

How to Cite

Ongarbayeva, N. S., Klivleyeva, N. G., Saktaganov, N. T., Kalkozhayeva, M. K., Glebova, T. I., Lukmanova, G. V., Baimukhametova, A. M., Mustafin, M. K., Yerdenov, S. G., Mustafin, B. M., Оspanov G. K., & Webby, R. J. (2020). Influenza virus circulation in swine populations in various regions of the Republic of Kazakhstan (2018 - 2019). Experimental Biology, 80(3), 70–80. https://doi.org/10.26577/eb-2019-3-b7

Issue

Vol. 80 No. 3 (2019): Experimental Biology

Section

VIROLOGY