Screening of micromycete strains that are promising for crop growth promotion
DOI:
https://doi.org/10.26577/eb-2019-3-b4Abstract
One of the current trends in the development of sustainable agriculture is the microbial biotechnology, contributing to the greening of agricultural production. Microorganisms are able to promote the growth and development of plants through various direct and indirect mechanisms. The interest in such microorganisms is associated with the possibility of using them to increase the yield of agricultural crops, improve the quality of agricultural products, restore soil fertility, etc. In the present study we examined the biotechnologically valuable properties of 848 micromycete strains (653 filamentous fungi and 195 yeasts) isolated from the agrocenoses of grain, forage and oilseeds of Kazakhstan. The strains capable of producing phyothhormones auxins and hydrolytic enzymes, the isolates with phosphate-mobilizing ability, cellulolytic and antagonistic activities, as well as tolerant to heavy metals, were identified. As a result of the screening, 44 strains that demonstrated the highest activity and possess several valuable properties were selected. Seed pre-treatment with these strains (in 35 cases out of 45) led to the promoting effect expressed in enhancing seed germination and increasing morphometric parameters of 14-day-old barley seedlings. As a result, 35 strains were selected for further detailed study of their properties and the possibility of application them for crop growth promotion.
Key words: micromycetes, plant growth promoting activity, phyothhormones, phosphate-mobilization, antagonism
References
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2. Dixon G.R., Tilston E.L. Soil microbiology and sustainable crop production. Netherlands: Springer; 2010.
3. Kim Y.C., Leveau J., McSpadden Gardener B.B., Pierson E.A. (2011) The multifactorial basis for plant health promotion by plant-associated bacteria. Appl Environ Microbiol., vol. 77(5), pp. 1548–55.
4. Vessey J.K. (2003) Plant growth promoting rhizobacteria as biofertilizers. Plant Soil, vol. 255, pp.571–86.
5. Martínez-Viveros O., Jorquera M.A., Crowley D.E., Gajardo G., Mora M.L. (2010) Mechanisms and practical considerations involved in plant growth promotion by rhizobacteria. J. Soil Sci Plant Nutr., vol. 10(3), pp. 293–319.
6. Vessey J.K. (2003) Plant growth promoting rhizobacteria as biofertilizers. Plant Soil, vol. 255, pp. 571–86.
7. Spaepen S. (2015) Plant Hormones Produced by Microbes. In: Lugtenberg B. (eds) Principles of Plant-Microbe Interactions. Springer, Cham
8. Shi T.Q., Peng H., Zeng S.Y., Ji R.Y., Shi K., Huang H., Ji X.J. (2017) Microbial production of plant hormones: Opportunities and challenges. Bioengineered., vol. 8(2), pp. 124–128
9. Adesemoye A., Torbert H., Kloepper, J. (2008). Enhanced plant nutrient use efficiency with PGPR and AMF in an integrated nutrient management system. Can. J. Microbiol., vol. 54, pp. 876–86.
10. Richardson A.E., Barea J.M., McNeill A.M., Prigent-Combaret C. (2009) Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant Soil, vol. 321, pp. 305–339.
11. Budania K., Yadav J. (2014) Effects of PGPR blended biochar and different levels of phosphorus on yield and nutrient uptake by chickpea. Ann. Agric. Bio Res., vol. 19, pp. 408–412.
12. Satyavir S.S., Phour M., Choudhary S.R.,Chaudhary D. (2014) Phosphorus cycling prospects of using rhizosphere microorganisms for improving phosphorus nutrition of plants. Geomicrobiol. Biogeochem., vol. 39, pp.199-237
13. Johri A.K., Oelmüller R., Dua M., Yadav V., Kumar M., Tuteja, N., et.al. (2015) Fungal association and utilization of phosphate by plants: success, limitations, and future prospects. Frontiers in microbiology, vol. 6, pp. 984.
14. Meena K.K., Sorty A.M., Bitla U.M., Choudhary K., Gupta P., Pareek, A. et.al. (2017) Abiotic Stress Responses and Microbe-Mediated Mitigation in Plants: The Omics Strategies. Frontiers in plant science, vol. 8, pp. 172.
15. Enebe M.C., Babalola O.O. (2019) The impact of microbes in the orchestration of plants’ resistance to biotic stress: a disease management approach. Appl Microbiol Biotechnol., vol. 103, pp. 9.
16. Rajkumar M, Sandhya S, Prasad M.NV, Freitas H. (2012) Perspectives of plant-associated microbes in heavy metal phytoremediation. Biotechnol Adv, vol. 30, pp.1562–1574
17. Compant S, Duffy B, Nowak J, Clément C. (2005) Use of plant growth-promoting bacteriafor biocontrol of plant diseases: principles, mechanisms of action, and futureprospects. Appl Environ Microbiol., vol. 71(9), pp. 4951–9.
18. Narayanasamy P. (2013) Mechanisms of Action of Fungal Biological Control Agents. In: Biological Management of Diseases of Crops. Progress in Biological Control, vol 15. Springer, Dordrecht
19. Whipps J.M. (2001) Microbial interactions and biocontrol in the rhizosphere. J Exp Bot., vol.52, pp.487- 511.
20. Choudhary D.K., Prakash A., Johri, B.N. (2008). Induced systemic resistance (ISR) in plants: mechanism of action. Indian journal of microbiology, vol. 47(4), pp. 289–297.
21. Pieterse C.M., Zamioudis C., Berendsen R.L., Weller D.M., Van Wees S.C., Bakker P.A. (2014) Induced systemic resistance by beneficial microbes. Annu Rev Phytopathol., vol. 52, pp. 347-75.
22. Glickmann E., Dessaux Y. (1995) A critical examination of the specificity of the Salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Applied and environmental microbiology, vol. 61(2), pp. 793–796.
23. Jayadi M., Baharuddin, Ibrahim B. (2013) In vitro selection of rock phosphate solubility by microorganism from Ultisols in South Sulawesi, Indonesia. American Journal of Agriculture and Forestry, vol. 1(4), pp. 68-73
24. Yoon J.H., ParkтJ.E., Suh D.Y., Hong S.B., Ko S.J., Kim S.H. (2007) Comparison of dyes for easy detection of extracellular cellulases in fungi. Mycobiology, vol. 35(1), pp. 21–24
25. Oladipo O.G., Awotoye O.O., Olayinka A., Bezuidenhout C.C., Maboeta M.S. (2018) Heavy metal tolerance traits of filamentous fungi isolated from gold and gemstone mining sites. Braz J Microbiol., vol. 49(1), pp. 29-37.
26. Collins C., Grange J., Lyne P. Microbiological Methods. – 8th Edition. - London:Hodder Arnold, 2004. – 480 p.
27. Ariffin Z.Z., Ahmad M.S., Pepi R., Noor Z.M. (2016) Proteolytic fungi from virgin forest. Jurnal Teknologi, vol. 78(6-7), pp. 37-41
28. Saryono, Piska F, Sari N., Pratiwi N.W., Ardhi A. (2018) Morphological identification and hydrolytic enzyme producing abilities of fungi associated with wilting banana plants (Musa sp.). Res J Chem Environ., pp. 79-86.
29. Spaepen S., Vanderleyden J., Remans R. (2007) Indole-3-acetic acid in microbial andmicroorganism-plant signaling. FEMS Microbiol., vol. 31, pp. 425–48.
30. Schulz S., Brankatschk R., Dumig A., Kogel-Knabner I., Schloter M., Zeyer J. (2013) The role of microorganisms at different stages of ecosystem development for soil formation. Biogeosciences, vol.10, pp. 3983–9
31. Khalid M., Yang W.J., Kishwar N., Rajput Z. I., Arijo, A. G. (2006). Study of cellulolytic soil fungi and two nova species and new medium. Journal of Zhejiang University. Science. B, vol. 7(6), pp. 459–466.
32. Gadd, G.M. (1992) Metals and microorganisms: A problem of definition. FEMS Microbiol Lett., vol.100 (1-3), pp. 197-204.
33. Savary S., Ficke A., Aubertot J., Hollier C. (2012). Crop losses due to diseases and their implications for global food production losses and food security. Food Security, vol. 4(4), pp. 519-537.
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How to Cite
Brazhnikova, Y. V., Mukasheva, T. D., & Ignatova, L. V. (2020). Screening of micromycete strains that are promising for crop growth promotion. Experimental Biology, 80(3), 40–47. https://doi.org/10.26577/eb-2019-3-b4
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