Extraction of resveratrol with the enzyme preparation Pectinol F-RKM 0719 produced by the strain Aspergillus awamori F-RKM 0719
DOI:
https://doi.org/10.26577/eb-2018-4-1367Abstract
Resveratrol is natural phytoalexin with pronounced therapeutic and prophylactic properties, it is part of the grape skin composition. However, due to the fact that it is in a bound state, the use of it by organism significantly reduced. Therefore, for the extraction of resveratrol is a need to use the enzyme preparations. The purpose of this study is maximize the recovery of resveratrol from the grape skin.
During ripening of grape, the mass concentration of resveratrol increases, and the maximum value is reached in the Caberno Sauvignan variety - 467 mg / dm3. In all experiments on the extraction of resveratrol from grape squeeze by the strain Aspergillus awamori F-RKM 0719 and the enzyme preparation Pectinol F-RKM 0719 an increase in the quantity of resveratrol is observed. In experiments with Caberno Sauvignan, the amount of resveratrol was maximal and increased from 392.66 control sample, when using the strain Aspergillus awamori F-RKM 0719 - 497.66, and when resveratrol was extracted by the enzyme preparation Pectinol F-RKM 0719 - 699.62 mg / dm3 .
Studies have shown the possibility of using the Caberno Sauvienne grape variety and the enzyme preparation Pectinol F-RKM 0719, which significantly increases the amount of extracted resveratrol.
Keywords: resveratrol, enzyme preparation Pectinol F-RKM 0719, strain Aspergillus awamori F-RKM 0719, fermented juice.
References
2 Бекетов А. Н. Виноградная лоза // Энциклопедический словарь Брокгауза и Ефрона : в 86 т. (82 т. и 4 доп.). — 2002. — С. 20—31. — 222 с.
3 Блиева, Р.К. Новый метод длительного культивирования и селекции продуцентов ферментов // Микробные биотехнологии: фундаментальные и прикладные аспекты: сб. науч. раб. – Минск: Изд-во «Белорусская наука»,- 2013. – С. 29-39.
4 Pedrolli D.B., Monteiro A.C., Gomes E., Carmona E.C. Pectin and pectinases: production, characterization and industrial application of microbial pectinolytic enzymes // Open Biotechnology Journal. - 2009. - Vol.3. - P. 9-18.
5 Донченко Л.В., Фирсов Г.Г. Пектин: основные свойства, производство и применение: монография. - Москва: ДеЛи принт, - 2007. - 276 с.
6 Мурадов М.С., Даудова Т.Н., Рамазанова Л.А. Экстракция красящих веществ из растительного сырья // Хранение и переработка сельхозсырья. - 2000. №4. - С.21-27.
7 Toaldo I.M., Gois J.S., Fogolari O., Hamann D., Borges D.L.G., Bordignon-Luiz M.T. Phytochemical polyphenol extraction and elemental composition of Vitis labrusca L. grape juices through optimization of pectinolytic activity // Food and Bioprocess Technol. –2014. –V. 7. №9. –P. 2581-2594.
8 Sandri I.G., Fontana R.C., Barfknechk D.M., Silveira M.M. Clarification of fruit juices by fungal pectinases // Food Science and Technology. - 2011. – V. 44. – P. 2217-2222.
9 Mieszczakowska-Frąс M., Markowski J., Zbrzeźniak M., Płocharski W. Impact of enzyme on quality of blackcurrant and plum juices // Food Science and Technology. - 2012. – V. 49. – P. 251-256.
10 Мехузла Н.Н., Щербаков С.С. Использование новых ферментных препаратов гидролитического действия при производстве природно-полусладких вин // Материалы III Международной научно-технической конференции, посвященной 80-летию ГОУВПО «Воронежская государственная технологическая академия» - Воронеж: изд-во ВГТА. – 2009. – С. 464-466.
11 Агеева Н.М., Маркосов В.А. Процианидины красных сортов винограда и красных столовых вин // Научные труды ГНУ СКЗНИИСиВ. – 2013. – Т. 4. – С. 195-202.
12 Ducasse M.A., Williams P., Canal-Llauberes R.M., Mazerolles G., Cheynier V., Doco Th. Effect of macerating enzymes on the oligosaccharide prefiles of Merlot red wines // J. Agr. Food Chem. – 2011.- Vol. 59. № 12. - Р.6558-6567.
13 Гнетько Л.В., Белявцева Т.А., Агеева Н.М. Влияние ферментных препаратов на ароматический комплекс яблочных сброженно-спиртованных соков // Научные труды Государственного научного учреждения Северо-Кавказского зонального научно-исследовательского института садоводства и виноградарства Российской академии сельскохозяйственных наук.- 2013. - Т. 4. - С. 85-91.
14 Чернович Ф.Л. Влияние комплексов эндогенных ферментов на время контактирования виноградного сусла с мезгой // Прикл. биохим. и микробиол. – 2007. –.№ 2. - С. 115-120.
15 Мартисян Е.А., Жуков Н.А. Оптимизация стадии ферментативной обработки плодово-ягодного сырья при получении спиртованных соков // Всероссийская научно-техническая конференция «Наука – производство – технология - экология».- 2005. –Т. 2, Киров, 2005. - С.194
16 Dzhakasheva M., Kedelbaev B., Peter Lieberzeit, Mutalieva B., Elemanova Zh., Esimova A., Abubakirova A. Selection and characteristic of the Aspergillus аwamori mutant strain – pectinase producers // ICITE – 2016: III international conference «Industrial technologies and engineering». - Shymkent, 2016.- P. 256-260
17 Мультиэнзимная композиция для виноделия: пат. на полезн. мод. РК: МПК C12N9/14 Джакашева М.А.; Кедельбаев Б.Ш.; Сапарбекова А.А.; Ашир А.К.; заявитель и патентообладатель ЮКГУ им. М. Ауэзова. - № 016/0147.2; заявл. 18.03.2016;
18 Pedrolli D.B., Monteiro A.C., Gomes E., Carmona E.C. Pectin and pectinases: production, characterization and industrial application of microbial pectinolytic enzymes // Open Biotechnology Journal. - 2009. - Vol.3. - P. 9-18.
19 Горышкина Т. К. Бахчевые культуры // Т. К. Горышкина, М. Е. Игнатьева. – Киев : Аграрная наука, 2000.– 149 с.
20 БopиcoвaA. Г. Poд 921. Гpaнaт — Punica // ФлopaCCCP. В 30 т.— М.:-1999. — Т. XV. — C. 553. — 742 c.
21 Шобингер У. (ред.). Фруктовые и овощные соки: научные основы и технологии / пер. с нем. под общ. науч. ред. А.Ю. Колеснова, Н.Ф. Берестеня и А.В. Орещенко. - СПб.: Профессия, - 2004. – 640с.
22 Сарафанова Л.А. Применение пищевых добавок в индустрии напитков. - СПб.: Профессия, - 2007. – 240с.
23 Пaнкoвa Н.В. (peд.) Иннoвaциoнныe тexнoлoгии в oблacти пищeвыx пpoдyктoв и пpoдyкции oбщecтвeннoгo питaния фyнкциoнaльнoгo и cпeциaлизиpoвaннoгo нaзнaчeния. Кoллeктивнaя мoнoгpaфия. — CПб.:Изд-вo «ЛEМA», - 2012. — 314 c.
24 Oлeфиpoвa, A.П. Opгaнoлeптичecкaя oцeнкa пищeвыx пpoдyктoв: yчeбнo-пpaктич. Пocoбиe. - Улaн-Удe: Изд-вoВCГТY, - 2010. - 192c.
25 Гopбyнoвa, В.П. Пopядoк и cpoки пpиeмки тoвapoв пo кoличecтвy и кaчecтвy. - М.:Экcпepтнoe бюpo, - 2008. - 98 c.
References:
Ageeva N.M., Markosov V.A. (2013) Procyanidi krasnyh sortov vinograda e krasnyh stolovyh vin [Procyanidins of red grape varieties and red table wines] Scientific works of the GNU SKZNIISiV. vol 4, pp. 195-202.
Azad, A.K. M., Ali, M. A., Akter, M.S., Rahman,M.J., Maruf Ahmed, M. (2014). Isolation and Characterization of Pectin Extracted from Lemon Pomace during Ripening. Journal of Food and Nutrition Sciences. Vol. 2, No. 2, pp. 30-35.
Beketov A.N. (2002)Vinogradnaya loza [Grapevine]. Encyclopedic Dictionary of Brockhaus and Efron: vol. 86, pp. 20–31.
Blieva R.K. (2013) Novye metod dlitelnogo kultivirovaniya e selekcii producenton fermentov [A new method of long-term cultivation and breeding of enzyme producers] Microbial biotechnologies: fundamental and applied aspects: Coll. scientific works. Minsk. Publishing House "Belarusian Science". pp. 29-39/
Borisova A.G. (1999) Rod 921. Granat-- Punica [Granat – Punica] Flora of USSR vol. 30 , pp 553 - 742 .
Chernovich F.L. (2007) Vliyanie kompleksov endogennyh fermentov ns vremi kontaktirovania vinogradnogo susla s mezgoy [Influence of complexes of endogenous enzymes on the time of contact of grape must with pulp] Application.biochemical and microbiol. vol. 2, pp. 115-120.
Dzhakasheva, M., Kedelbaev, B., Peter Lieberzeit, Mutalieva, B., Elemanova, Zh., Esimova, A., Abubakirova, A. (2016) Aspergillus awamori mutant strain pectinase producers, III international conference Industrial technologies and engineering. Shymkent, pp. 256-260.
Donchenko L.V., Firsov G.G. (2007) Pektin: osnovnye svoystva, proisvodstvo e primenenie [Pectin: basic properties, production and use] monograph. -Moscow: DeLi Print, pp. 276.
Ducasse M.A., Williams P., Canal-Llauberes R.M., Mazerolles G., Cheynier V., Doco Th.
(2011) Effect of macerating enzymes on the oligosaccharide prefiles of Merlot red wines // J. Agr. Food Chem. vol. 59, no 12, pp. 6558-6567.
Gnetko L.V., Belyavtseva T.A., Ageeva N.M. (2013) Vlianie fermentnyh preparatov na aromaticheski kompleks sbrosheno spirtovyh sokov [The influence of enzyme preparations on the aromatic complex of apple fermented-alcoholized juices] Scientific works of the State Scientific Institution of the North Caucasus Zonal Scientific Research Institute of Horticulture and Viticulture of the Russian Academy of Agricultural Sciences. vol. 4, pp. 85-91.
Gobunova, V.P. (2008) Poraydok e sroki priemki tovarov po kolichestvu e kachestvu [The requirement and time of the good traces in quality and quantity]. The EXPERT BUREAU, pp. 98.
Goryshkina, T. K. (2009) Bahchevye kultury [Watermelon culture] Kiev. Agrarian science, pp. 149.
Kedelbayev B., Saparbekov A.A . Ashir A.K. Dzhakasheva M.A. (2016) Multienzyme composition for winemaking: Pat. on useful Maud. RK: IPC C12N9 / 14 MA. applicant and patent holder M. Auezov SKSU no 016 / 0147.
Martisyan E.A., Zhukov N.A. ( 2005) Optimizacia stadiya fermentnoy obrabotki plodovo yagodnogo Syria pri poluchenii spirtovonyh sokov [Optimization of the stage of enzymatic processing of fruit and berry raw materials in the production of alcoholized juices]All-Russian Scientific and Technical Conference "Science - production - technology - Maznev N.I. (2004) Arbuz syedobnyi [Edible watermelon]. Encyclopedia of medicinal plants. 3rd ed., Corr. and add. Martin, 496 pp.
Mekhuzla N.N., Shcherbakov S.S. (2009) Ispolzovanie novyh fermentnyh preparatov gidroliticheskogo deystvya pri proizvodstve prirodno polusladkih vin. [The use of new enzymatic preparations of hydrolytic action in the production of naturally semi-sweet wines] Proceedings of the III International Scientific and Technical Conference dedicated to the 80th anniversary of the Voronezh State Technological Academy, Voronezh: VGTA publishing house.pp. 464-466.
Mieszczakowska-Frąs M., Markowski J., Zbrzeźniak M., Płocharski W. (2012) Impact of the quality of the blackcurrant and plum juices. Food Science and Technology. vol. 49. pp. 251-256.
Muradov MS, Daudova T.N., Ramazanova L.A. (2000) Exstrakciia krasashih veshestv iz rastitelnogo syrya [Extraction of coloring matter from vegetable raw materials] Storage and processing of agricultural raw materials. vol. 4, pp.21-27.
Pankova N.V. (ed.) (2012) Inovacionye technologie v oblasti pishevyh produktov e produkcii obshestvennogo pitania funkcionalnogo e specializirovannogo naznachenia [Innovative technologies in the field of foodstuffs and products of general nutrition of functional and specialty equipment]. Collective monograph. St. Petersburg: Publishing House "LEMA", pp. 314.
Oleframes A.P. (2010) Organolepticheskaya ocenka pishevyh productov [Organizational estimate of food products]: practical book. Ulan-Ude, pp.192.
Pedrolli D.B., Monteiro A.C., Gomes E., Carmona E.C. (2009) Pectin and pectinases: production, characterization and industrial application of microbial pectinolytic enzymes // Open Biotechnology Journal. vol.3, pp 9-18.
Sandri I.G., Fontana R.C., Barfknechk D.M., Silveira M.M. (2011) Clarification of fruit juices by fungal pectinases // Food Science and Technology. vol. 44, pp. 2217-2222.
Sarafanova L.A. (2007) Primenenie pishevyh dobavok v industrii napitkov [The use of food additives in the beverage industry] SPb, Profession, pp. 240.
Schobinger U. (ed.). (2004) Fruktovye i ovoshnye soki [Fruit and vegetable juices] scientific foundations and technologies / transl. from German by total scientific ed. A.Yu. Kolesnova, N.F. Berestenya and A.V. Oreschenko. SPb . Profession, pp. 640.
Toaldo, I.M., Gois, J.S., Fogolari, O., Hamann, D., Borges, D.L.G., Bordignon-Luiz, M.T. (2014) Phytochemical polyphenol extraction and elemental composition of the Vitis labrusca L. grape juices through pectinolytic activity // Food and Bioprocess Technol. vol. 7, no 9. pp. 2581-2594.