Microclonal propagation in vitro of commercial varieties of rose
DOI:
https://doi.org/10.26577/EB-2017-4-1300Keywords:
роза (Rosa L.), микроклональное размножение, in vitro, регуляторы ростаAbstract
To propagate the seedling of hybrid and commercial varieties roses of Kazakhstan breeding, adapted to the arid regions of Western Kazakhstan, the microclonal propagation in vitro technology was used. For introduce hybrid roses in in vitro culture, the nodal segments with axillary buds, as well as the internode segments taken from plants actively growing in Mangishlak experimental botanic garden, were isolated in aseptic conditions. Sterilization by treating the explants with a 10% solution of hydrogen peroxide for 8 minutes was the most effective, than its treatment by 70% ethanol (20 sec.) and subsequent 5-minute exposure in 5% sodium hypochlorite. Due to this method of disinfection, the yield of viable explants was maximal - 68.4%. Cloning of roses was carried out on different variants of Murashige-Skug medium modified by phytohormones. The greatest number of microclones was obtained on MS medium containing 3 mg/l BAP and 0.5 mg/l NAA. Ascorbic acid (0.5 mg/l) was added into MC medium as an antioxidant. For rooting was used MC medium with 2 mg/l IAA. The multiplication rate in this case was 4. This optimized protocol is recommended for the introduction of commercial varieties of roses, as well as for gardening of the Caspian region.
References
1. Бессчетнова М.В., Михнева Т.Н. Розы. – Алма-Ата: Кайнар. -1979. - 120 с.
2. Candi F.A., Kazaz О. Biotechnology of roses: progress and future peospects // Suleyman Dermirel Univer. Orman Fakultesi Dergisi (Seri:A). -2009. - Vol.1. -P.167-183.
3. Das P. Mass cloning of Rose and Mussaenda, popular garden plants, via somatic embryogenesis // Hort.Sci. -2010. – Vol. 37. –P. 70-78.
4. Зонтиков Д.Н., Зонтикова С.А. Особенности клонального микроразмножения декоративных сортов Rosa hybrid // Вестник КГУ им.Н.А.Некрасова – 2011. – № 5. –P. 56-63
5. Khosravi P., Kermani M.J., Nematzadeh G.A., Bihamta M.R. A protocol for mass production of Rosa hybridacv. Iceberg through in vitro propagation // Iranian J. of Biotechnology. – 2007. - Vol. 5. –P. 100-104.
6. Kim S.W., Oh M.J., Liu J.R. Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose // Plant Biotechnology Reports. -2009. - Vol. 3. - P. 199-203.
7. Krosh-Khui M., De Silva J.A.T. In vitro culture of the Rosa species // Floriculture, Ornamental and Plant Biotechnology- 2006. - Vol. 2. – P.514-527.
8. Malabadi R.B., Van Staden J. Role of antioxidants and amino acids on somatic embryogenesis of Pinus patula. // In Vitro Cellular and Developmental Biology: Plant. – 2005. - Vol. 41. – P. 181–186.
9. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. // Physiologia Plantarum. – 1962. – Vol. 15. – P. 473-497.
10. Мухамбетжанов С.К., Мурсалиева В.К., Вечерко Н.А., Нам С.В. Оптимизация протоколов питательных сред для микроклонального размножения роз // Вестник КазНУ. Серия биологическая. – 2011. -№ 4. – C. 45-48.
11. Мухамбетжанов С.К., Нам С.В., Вечерко Н.А., Мурсалиева В.К. Факторы влияющие на рост и развитие роз in vitro // Биотехнология. Теория и практика. – 2010. - № 1. – C. 41-52.
12. Nak-Udom N., Kanchanapoom K., Kanchanapoom K. Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’) // Songklanakarin J. Sci. Technol. – 2009. – Vol. 31. –P. 583-586.
13. Nitish Kumar, Reddy M. In vitro Plant Propagation: a review // Journal of Forest Science. – 2011. - Vol. 27. – P. 61-72.
14. Pratar Kumar Pati, Siba Prasad Rath, Madhu Sharma, Anil Sood, Paramvir Singh Ahuja. In vitro propagation of rose - a review // Biotechnology Advances. – 2006. – Vol. 24. – P. 94-114.
15. Roy P.K., Mamum A.N.K., Ahmed G. In vitro plantlets regeneration of rose // Plant Cell, Tissue and Organ Culture. – 2004. - Vol.14. - P.149-154.
16. Ruzic D., Lazic T. Micropropagation as means of rapid multiplication of newly developed blackberry and black currant cultivars // Agriculturae Conspectus Scientificus. – 2006. – Vol. 71. – P. 149-153.
17. Сапаргали О., Нам С.В., Вечерко Н.А., Мухамбетжанов С.К., Мурсалиева В.К. Влияние генотипа на микроклональное размножение роз // Сб. тр. междунар.конф. «Актуальные проблемы ботанического ресурсоведения», Алматы. - 2010. - С.341-343
18. Saxena G., Banerjee S., Rahman L., Mallavarapu G.R., Sharma S., Kumar S. An efficient in vitro procedure for micropropagation and generatio of somaclones of rose scented Pelargonium // Plant Science. – 2000. – Vol. 29. –P. 133-140.
19. Smith R.H. Plant Tissue Culture: Techniques and Experiments. Academic Press. – 2000. - P. 31-46.
20. Stasolla C., Lam M.S.W., Yeung E.C. Exogenous applications of ascorbic acid enhance shoot apical meristem growth and induce shoot organogenesis in germinating white spruce Picea glauca somatic embryos // International Journal of Plant Sciences. – 2006. - № 167. – P. 429–436.
References
1. Besschetnova M.V., Mikhneva T.N. (1979) Rosi [Roses]. - Alma-Ata: Kainar, 120 p.
2. Candi F.A., Kazaz О. (2009) Biotechnology of roses: progress and future peospects. Suleyman Dermirel Univer. Orman Fakultesi Dergisi (Seri:A, vol.1, pp. 167-183.
3. Das P (2010) Mass cloning of Rose and Mussaenda, popular garden plants, via somatic embryogenesis. Hort.Sci., vol. 37, pp. 70-78.
4. Zontikov D.H., Zontikova S.A. (2011) Osobennosti klonalnogo microrazmnozhenia dekorativnih sortov Rosa hybrid [Features of clonal micropropagation ornamental varities of Rosa hybrid]. Byulleten KGU imeni N.A.Nekrasova, vol. 5, pp. 56-63.
5. Khosravi P., Kermani M.J., Nematzadeh G.A., Bihamta M.R. (2007) A protocol for mass production of Rosa hybridacv. Iceberg through in vitro propagation. Iranian J. of Biotechnology, vol. 5, pp.100-104.
6. Kim S.W., Oh M.J., Liu J.R. (2009) Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose. Plant Biotechnology Reports, vol. 3, pp. 199-203.
7. Krosh-Khui M., De Silva J.A.T. (2006) In vitro culture of the Rosa species. Floriculture, Ornamental and Plant Biotechnology, vol. 2, pp. 514-527.
8. Malabadi R.B., Van Staden J. (2005) Role of antioxidants and amino acids on somatic embryogenesis of Pinus patula. In Vitro Cellular and Developmental Biology: Plant, vol. 41, pp. 181–186.
9. Murashige T, Skoog F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, vol. 15, pp. 473-497.
10. Mukhambetzhanov S.K., Mursalieva V.K., Vecherko N.A., Nam S.V. (2011) Optimizacia protokolov pitatelnih sred dlja mikroklonalnogo razmnozhenia roz [Optimisation of protocol of nutrient medium for microclonal propagation of rose] Bulletin of Kazakh National University. Experimental Biology, vol. 4, pp. 45-48.
11. Mukhambetzhanov S.K., Nam S.V., Vecherko N.A., Mursalieva V.K. (2010) Faktori vlijajushie na rost i razvitie roz in vitro [The factors influence on growth and development of rose in vitro]. Biotechnology: theory and practice, vol. 1, pp. 41-52.
12. Nak-Udom N., Kanchanapoom K., Kanchanapoom K. (2009) Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’) Songklanakarin J. Sci. Technol., vol. 31, pp. 583-586.
13. Nitish Kumar, Reddy M. (2011) In vitro Plant Propagation: a review. Journal of Forest Science, vol. 27, pp. 61-72.
14. Pratar Kumar Pati, Siba Prasad Rath, Madhu Sharma, Anil Sood, Paramvir Singh Ahuja (2006) In vitro propagation of rose - a review. Biotechnology Advances, vol. 24, pp. 94-114.
15. Roy P.K., Mamum A.N.K., Ahmed G. (2004) In vitro plantlets regeneration of rose. Plant Cell, Tissue and Organ Culture, vol. 14, pp. 149-154.
16. Ruzic D., Lazic T. (2006) Micropropagation as means of rapid multiplication of newly developed blackberry and black currant cultivars. Agriculturae Conspectus Scientificus, vol. 71, pp. 149-153.
17. Sapargali O., Nam S.V., Vecherko N.A., Mukhambetzhanov S.K., Mursalieva V.K. (2010) Influence of the genotype on the microclonal propagation of roses. Abstracts of international conference "Actual problems of botanical research", Almaty, pp. 341-343.
18. Saxena G., Banerjee S., Rahman L., Mallavarapu G.R., Sharma S., Kumar S. (2000) An efficient in vitro procedure for micropropagation and generatio of somaclones of rose scented Pelargonium. Plant Science, vol. 29, pp. 133-140.
19. Smith R.H. (2000) Plant Tissue Culture: Techniques and Experiments. Academic Press, pp. 31-46.
20. Stasolla C., Lam M.S.W., Yeung E.C. (2006) Exogenous applications of ascorbic acid enhance shoot apical meristem growth and induce shoot organogenesis in germinating white spruce Picea glauca somatic embryos. International Journal of Plant Sciences, vol. 167, pp. 429–436.
2. Candi F.A., Kazaz О. Biotechnology of roses: progress and future peospects // Suleyman Dermirel Univer. Orman Fakultesi Dergisi (Seri:A). -2009. - Vol.1. -P.167-183.
3. Das P. Mass cloning of Rose and Mussaenda, popular garden plants, via somatic embryogenesis // Hort.Sci. -2010. – Vol. 37. –P. 70-78.
4. Зонтиков Д.Н., Зонтикова С.А. Особенности клонального микроразмножения декоративных сортов Rosa hybrid // Вестник КГУ им.Н.А.Некрасова – 2011. – № 5. –P. 56-63
5. Khosravi P., Kermani M.J., Nematzadeh G.A., Bihamta M.R. A protocol for mass production of Rosa hybridacv. Iceberg through in vitro propagation // Iranian J. of Biotechnology. – 2007. - Vol. 5. –P. 100-104.
6. Kim S.W., Oh M.J., Liu J.R. Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose // Plant Biotechnology Reports. -2009. - Vol. 3. - P. 199-203.
7. Krosh-Khui M., De Silva J.A.T. In vitro culture of the Rosa species // Floriculture, Ornamental and Plant Biotechnology- 2006. - Vol. 2. – P.514-527.
8. Malabadi R.B., Van Staden J. Role of antioxidants and amino acids on somatic embryogenesis of Pinus patula. // In Vitro Cellular and Developmental Biology: Plant. – 2005. - Vol. 41. – P. 181–186.
9. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. // Physiologia Plantarum. – 1962. – Vol. 15. – P. 473-497.
10. Мухамбетжанов С.К., Мурсалиева В.К., Вечерко Н.А., Нам С.В. Оптимизация протоколов питательных сред для микроклонального размножения роз // Вестник КазНУ. Серия биологическая. – 2011. -№ 4. – C. 45-48.
11. Мухамбетжанов С.К., Нам С.В., Вечерко Н.А., Мурсалиева В.К. Факторы влияющие на рост и развитие роз in vitro // Биотехнология. Теория и практика. – 2010. - № 1. – C. 41-52.
12. Nak-Udom N., Kanchanapoom K., Kanchanapoom K. Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’) // Songklanakarin J. Sci. Technol. – 2009. – Vol. 31. –P. 583-586.
13. Nitish Kumar, Reddy M. In vitro Plant Propagation: a review // Journal of Forest Science. – 2011. - Vol. 27. – P. 61-72.
14. Pratar Kumar Pati, Siba Prasad Rath, Madhu Sharma, Anil Sood, Paramvir Singh Ahuja. In vitro propagation of rose - a review // Biotechnology Advances. – 2006. – Vol. 24. – P. 94-114.
15. Roy P.K., Mamum A.N.K., Ahmed G. In vitro plantlets regeneration of rose // Plant Cell, Tissue and Organ Culture. – 2004. - Vol.14. - P.149-154.
16. Ruzic D., Lazic T. Micropropagation as means of rapid multiplication of newly developed blackberry and black currant cultivars // Agriculturae Conspectus Scientificus. – 2006. – Vol. 71. – P. 149-153.
17. Сапаргали О., Нам С.В., Вечерко Н.А., Мухамбетжанов С.К., Мурсалиева В.К. Влияние генотипа на микроклональное размножение роз // Сб. тр. междунар.конф. «Актуальные проблемы ботанического ресурсоведения», Алматы. - 2010. - С.341-343
18. Saxena G., Banerjee S., Rahman L., Mallavarapu G.R., Sharma S., Kumar S. An efficient in vitro procedure for micropropagation and generatio of somaclones of rose scented Pelargonium // Plant Science. – 2000. – Vol. 29. –P. 133-140.
19. Smith R.H. Plant Tissue Culture: Techniques and Experiments. Academic Press. – 2000. - P. 31-46.
20. Stasolla C., Lam M.S.W., Yeung E.C. Exogenous applications of ascorbic acid enhance shoot apical meristem growth and induce shoot organogenesis in germinating white spruce Picea glauca somatic embryos // International Journal of Plant Sciences. – 2006. - № 167. – P. 429–436.
References
1. Besschetnova M.V., Mikhneva T.N. (1979) Rosi [Roses]. - Alma-Ata: Kainar, 120 p.
2. Candi F.A., Kazaz О. (2009) Biotechnology of roses: progress and future peospects. Suleyman Dermirel Univer. Orman Fakultesi Dergisi (Seri:A, vol.1, pp. 167-183.
3. Das P (2010) Mass cloning of Rose and Mussaenda, popular garden plants, via somatic embryogenesis. Hort.Sci., vol. 37, pp. 70-78.
4. Zontikov D.H., Zontikova S.A. (2011) Osobennosti klonalnogo microrazmnozhenia dekorativnih sortov Rosa hybrid [Features of clonal micropropagation ornamental varities of Rosa hybrid]. Byulleten KGU imeni N.A.Nekrasova, vol. 5, pp. 56-63.
5. Khosravi P., Kermani M.J., Nematzadeh G.A., Bihamta M.R. (2007) A protocol for mass production of Rosa hybridacv. Iceberg through in vitro propagation. Iranian J. of Biotechnology, vol. 5, pp.100-104.
6. Kim S.W., Oh M.J., Liu J.R. (2009) Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose. Plant Biotechnology Reports, vol. 3, pp. 199-203.
7. Krosh-Khui M., De Silva J.A.T. (2006) In vitro culture of the Rosa species. Floriculture, Ornamental and Plant Biotechnology, vol. 2, pp. 514-527.
8. Malabadi R.B., Van Staden J. (2005) Role of antioxidants and amino acids on somatic embryogenesis of Pinus patula. In Vitro Cellular and Developmental Biology: Plant, vol. 41, pp. 181–186.
9. Murashige T, Skoog F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, vol. 15, pp. 473-497.
10. Mukhambetzhanov S.K., Mursalieva V.K., Vecherko N.A., Nam S.V. (2011) Optimizacia protokolov pitatelnih sred dlja mikroklonalnogo razmnozhenia roz [Optimisation of protocol of nutrient medium for microclonal propagation of rose] Bulletin of Kazakh National University. Experimental Biology, vol. 4, pp. 45-48.
11. Mukhambetzhanov S.K., Nam S.V., Vecherko N.A., Mursalieva V.K. (2010) Faktori vlijajushie na rost i razvitie roz in vitro [The factors influence on growth and development of rose in vitro]. Biotechnology: theory and practice, vol. 1, pp. 41-52.
12. Nak-Udom N., Kanchanapoom K., Kanchanapoom K. (2009) Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’) Songklanakarin J. Sci. Technol., vol. 31, pp. 583-586.
13. Nitish Kumar, Reddy M. (2011) In vitro Plant Propagation: a review. Journal of Forest Science, vol. 27, pp. 61-72.
14. Pratar Kumar Pati, Siba Prasad Rath, Madhu Sharma, Anil Sood, Paramvir Singh Ahuja (2006) In vitro propagation of rose - a review. Biotechnology Advances, vol. 24, pp. 94-114.
15. Roy P.K., Mamum A.N.K., Ahmed G. (2004) In vitro plantlets regeneration of rose. Plant Cell, Tissue and Organ Culture, vol. 14, pp. 149-154.
16. Ruzic D., Lazic T. (2006) Micropropagation as means of rapid multiplication of newly developed blackberry and black currant cultivars. Agriculturae Conspectus Scientificus, vol. 71, pp. 149-153.
17. Sapargali O., Nam S.V., Vecherko N.A., Mukhambetzhanov S.K., Mursalieva V.K. (2010) Influence of the genotype on the microclonal propagation of roses. Abstracts of international conference "Actual problems of botanical research", Almaty, pp. 341-343.
18. Saxena G., Banerjee S., Rahman L., Mallavarapu G.R., Sharma S., Kumar S. (2000) An efficient in vitro procedure for micropropagation and generatio of somaclones of rose scented Pelargonium. Plant Science, vol. 29, pp. 133-140.
19. Smith R.H. (2000) Plant Tissue Culture: Techniques and Experiments. Academic Press, pp. 31-46.
20. Stasolla C., Lam M.S.W., Yeung E.C. (2006) Exogenous applications of ascorbic acid enhance shoot apical meristem growth and induce shoot organogenesis in germinating white spruce Picea glauca somatic embryos. International Journal of Plant Sciences, vol. 167, pp. 429–436.
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Published
2018-05-18
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BIOTECHNOLOGY
