Bioecological features of Penicillium aurantiogriseum Dierckx
167 61
Keywords:
species of fungi, DNА, RNA, PCR, sequencing, Penicillium aurantiogriseum Dierckx.Abstract
Under the influence of fungi affecting vegetables, with decreased yield and reduced storage period every year. Everyone knows the practical importance of studying the biological and ecological features of species and innovative methods for the determination of species of pathogenic fungi. There are given the results of molecular genetic and morphological study of the pathogen species of fungi green mold of onion (Allium cepa L.). When determining the taxonomic place morphological features are often giving the inaccurate result. For this purpose the identification of plant pathogenic Penicillium aurantiogriseum produced by Polymerase Chain Reaction (PCR). NS1 and NS4 primers were used to determine the gene encoding 18S rRNA. When determining the genetic sequence and within transcribed spacer 5.8S RNA encoding gene were used primers ITS1 and ITS4. For amplification of D1 / D2 domain of the gene 26S primers were used rRNA NL-1 and NL-4. They were made phylogenetic analysis of connection to cognate strains of microorganisms in comparison of the nucleotide sequences obtained by DNA sequencing. As a result of sequencing of the strain was defined views. Additionally performed studies to determine the macro- and microscopic features micromorphological strain.
References
Әдебиеттер
1. Bridge P.D., Hawksworth Z., Kozakiewicz A.H., Onions S., Paterson R.R., Sackin M.J., A. Sneath P.H. A reappraisal of the terverticillate penicillia using biochemical, physiological and morphological features // Numerical taxonomy. J. of General Microbiol. November. - 1989.- Vol. 135. – P. 2941-2966. DOI: 10.1099/00221287-135-11-2941
2. Byther R.S., Chastagner G.A. Diseases. In: The Physiology of Flower Bulbs, a Comprehensive Treatise on the Physiology and Utilization of Ornamental Flowering Bulbous and Tuberous Plants (Eds.: De Hertogh, A., Le Nard, M.) // Elsev. Amsterdam. -1993. - Р.71-100
3. Frisvad J.C., Samson R.A. Polyphasic taxonomy of Penicillium subgenus Penicillium: a guide to identification of food and air-borne terverticillate Pencillia and their mycotoxins // Stud. Mycol. -2004. – Vol. 49. –P. 1-173.
4. Davis R.M. Penicillium decay of garlic // In: Compendium of Onion and Garlic Diseases and Pests (Eds.: Schwartz, H.F., Mohan, S.K.). 2 nd Edn. APS Press, St. Paul, MN. – 2008. - P.52
5. Sumner D.R., Langston D.B., Seebold K.W. Blue Mold. // In: Compendium of Onion and Garlic Diseases and Pests (Eds.: Schwartz, H.F., Mohan, S.K.). 2 nd Edn. APS Press, St. Paul, MN. – 2008. - P. 52-53.
6. Andersen B., Frisvad J.C. Characterization of Alternaria and Penicillium Species from similar substrata based on growth at different temperature, pH and water activity // Syst. Appl. Microbial. - - 2002. - Vol. 25. – P.162-172.
7. Nagvi S.A.M.H. Diseases of Fruits and Vegetables: Diagnosis and Management. – USA.: Kluwer Academic Publishers, 2004. -537 c. ISBN 1-4020–1823-1.
8. Бекдаирова К.Ж. Биохимическая характеристика чеснока и лука в процессе вегетации и хранения: Автореферат дис. на соискание ученой степени кандидата биологических наук. (093) / АН КазССР. Объедин. учен. совет ин-тов почвоведения, ботаники, микробиологии вирусологии. 1971 - Алма-Ата. - 28 с.
9. Буренин В.И. Овощи родник здоровья: 3-е изд., перераб. и доп. - Л.: Лениздат, 1990.
-255 с.
10. Kalb T.J., Mavlyanova R.F. Vegetable production in Central Asia: status and perspectives. Proceedings of the workshop. The World Vegetable Center. - Shanhua, Taiwan: AVRDC, 2005. - P.134. ISBN 92-9058-139.
11. Duduk N., Vasiс M., Vico I. First Report of Penicillium polonicum Causing Blue Mold on Stored Onion (Allium cepa) in Serbia. // Plant Dis. – 2014. – Vol. 98, No. 10. – P. 144-145. DOI:10.1094/PDIS-05-14-0550-PDN.
12. Overy D.P., Frisvad J.C., Steinmeier U., Thrane U. Clarification of the agents causing blue mold storage rot uponvarious flower and vegetable bulbs: implicationsfor mycotoxin contamination. // Postharvest Biol. and Techn.- 2005. – Vol. 35. P. 217–221. DOI:10.1016/j.postharvbio.2004.08.001.
13. Weising K., Nybom H., Wolff K., Meyer W. In: DNA Fingerprinting in Plants and Fungi. – USA.: CRC Press, Boca Raton, 1995. – P. 336. ISBN : 0849389208
14. Salybekova N.N., Abdrassulova Zh.T., Kuzhantaeva Zh.Zh., Berdimuratova Zh.B., Babaeva G.A., Serzhanova A.E. Biological features of fungi susceptible varieties Allium Cepa L. // European Conference on Innovations in Technical and Natural Sciences, 6th International scientific conference 4th April, Vienna, Austria. - 2015. - P.17-27.
References
1. Bridge PD, Hawksworth DL, Kozakiewicz Z, Onions AH, Paterson RM, Sackin MJ, Sneath P. H (1989) A reappraisal of the terverticillate penicillia using biochemical, physiological and morphological features I. Numerical taxonomy. Journal of General Microbiology, November. 135: 2941-2966. DOI: 10.1099/00221287-135-11-2941
2. Byther RS, Chastagner GA (1993) Diseases. In: The Physiology of Flower Bulbs, a Comprehensive Treatise on the Physiology and Utilization of Ornamental Flowering Bulbous and Tuberous Plants (Eds.: De Hertogh, A., Le Nard, M.). Elsevier, Amsterdam. pp.71-100.
3. Frisvad JC, Samson RA (2004) Polyphasic taxonomy of Penicillium subgenus Penicillium: a guide to identification of food and air-borne terverticillate Pencillia and their mycotoxins. Stud. Mycol, 49: 1-173.
4. Davis RM (2008) Penicillium decay of garlic. In: Compendium of Onion and Garlic Diseases and Pests (Eds.: Schwartz, H.F., Mohan, S.K.). 2 nd Edn. APS Press, St. Paul, MN. p.52.
5. Sumner DR, Langston DB, Seebold KW (2008) Blue Mold. In: Compendium of Onion and Garlic Diseases and Pests (Eds.: Schwartz, H.F., Mohan, S.K.). 2 nd Edn. APS Press, St. Paul, MN. pp. 52-53.
6. Andersen B, Frisvad JC (2002) Characterization of Alternaria and Penicillium Species from similar substrata based on growth at different temperature, pH and water activity. Syst. Appl. Microbial. 25: 162-172.
7. Nagvi SAMH (2004) Diseases of Fruits and Vegetables: Diagnosis and Management. – USA, Kluwer Academic Publishers, -Р. 537. ISBN 1-4020–1823-1.
8. Bekdairova KZh (1971) Biochemical characterization of garlic and onions in the process of growing and storage, Almaty. Abstract dis. for the degree of candidate of biological sciences. (093) [Biochimicheskaya charakteristica chesnoka i luka v prosesse vegetasii i chranenya] KazSSR. Combine. scientists. Council Institute Comrade soil science, botany, microbiology, virology. – P.28. (In Russian)
9. Burenin VI (1990) Vegetable spring health: [Ovoshi rodnik zdorovia] 3rd ed., Revised. and ext. - L., Lenizdat, -P.255. ISBN 5-289-00741-5. (In Russian)
10. Kalb TJ, Mavlyanova RF (2005) Vegetable production in Central Asia: status and perspectives. Proceedings of the workshop. The World Vegetable Center. Shanhua, Taiwan. AVRDC, - P.134 ISBN 92-9058-139
11. Duduk N, Vasiс M, Vico I. (2014) First Report of Penicillium polonicum Causing Blue Mold on Stored Onion (Allium cepa) in Serbia. Plant Dis., 98 (10): 144-145. DOI:10.1094/PDIS-05-14-0550-PDN.
12. Overy DP, Frisvad JC, Steinmeier U, Thrane U. (2005) Clarification of the agents causing blue mold storage rot uponvarious flower and vegetable bulbs: implicationsfor mycotoxin contamination. Postharvest Biol. and Techn. 35. pp. 217–221. DOI:10.1016/j.postharvbio.2004.08.001.
13. Weising K, Nybom H, Wolff K, Meyer W (1995) In: DNA Fingerprinting in Plants and Fungi. CRC Press, Boca Raton, Florida, USA. – P. 336
14. Salybekova NN, Abdrassulova ZhT, Kuzhantaeva ZhZh, Berdimuratova ZhB, Babaeva GA, Serzhanova AE (2015) Biological features of fungi susceptible varieties Allium сepa L. // European Conference on Innovations in Technical and Natural Sciences 6th International scientific conference 4th April, Vienna, Austria. pp. 17-27.
1. Bridge P.D., Hawksworth Z., Kozakiewicz A.H., Onions S., Paterson R.R., Sackin M.J., A. Sneath P.H. A reappraisal of the terverticillate penicillia using biochemical, physiological and morphological features // Numerical taxonomy. J. of General Microbiol. November. - 1989.- Vol. 135. – P. 2941-2966. DOI: 10.1099/00221287-135-11-2941
2. Byther R.S., Chastagner G.A. Diseases. In: The Physiology of Flower Bulbs, a Comprehensive Treatise on the Physiology and Utilization of Ornamental Flowering Bulbous and Tuberous Plants (Eds.: De Hertogh, A., Le Nard, M.) // Elsev. Amsterdam. -1993. - Р.71-100
3. Frisvad J.C., Samson R.A. Polyphasic taxonomy of Penicillium subgenus Penicillium: a guide to identification of food and air-borne terverticillate Pencillia and their mycotoxins // Stud. Mycol. -2004. – Vol. 49. –P. 1-173.
4. Davis R.M. Penicillium decay of garlic // In: Compendium of Onion and Garlic Diseases and Pests (Eds.: Schwartz, H.F., Mohan, S.K.). 2 nd Edn. APS Press, St. Paul, MN. – 2008. - P.52
5. Sumner D.R., Langston D.B., Seebold K.W. Blue Mold. // In: Compendium of Onion and Garlic Diseases and Pests (Eds.: Schwartz, H.F., Mohan, S.K.). 2 nd Edn. APS Press, St. Paul, MN. – 2008. - P. 52-53.
6. Andersen B., Frisvad J.C. Characterization of Alternaria and Penicillium Species from similar substrata based on growth at different temperature, pH and water activity // Syst. Appl. Microbial. - - 2002. - Vol. 25. – P.162-172.
7. Nagvi S.A.M.H. Diseases of Fruits and Vegetables: Diagnosis and Management. – USA.: Kluwer Academic Publishers, 2004. -537 c. ISBN 1-4020–1823-1.
8. Бекдаирова К.Ж. Биохимическая характеристика чеснока и лука в процессе вегетации и хранения: Автореферат дис. на соискание ученой степени кандидата биологических наук. (093) / АН КазССР. Объедин. учен. совет ин-тов почвоведения, ботаники, микробиологии вирусологии. 1971 - Алма-Ата. - 28 с.
9. Буренин В.И. Овощи родник здоровья: 3-е изд., перераб. и доп. - Л.: Лениздат, 1990.
-255 с.
10. Kalb T.J., Mavlyanova R.F. Vegetable production in Central Asia: status and perspectives. Proceedings of the workshop. The World Vegetable Center. - Shanhua, Taiwan: AVRDC, 2005. - P.134. ISBN 92-9058-139.
11. Duduk N., Vasiс M., Vico I. First Report of Penicillium polonicum Causing Blue Mold on Stored Onion (Allium cepa) in Serbia. // Plant Dis. – 2014. – Vol. 98, No. 10. – P. 144-145. DOI:10.1094/PDIS-05-14-0550-PDN.
12. Overy D.P., Frisvad J.C., Steinmeier U., Thrane U. Clarification of the agents causing blue mold storage rot uponvarious flower and vegetable bulbs: implicationsfor mycotoxin contamination. // Postharvest Biol. and Techn.- 2005. – Vol. 35. P. 217–221. DOI:10.1016/j.postharvbio.2004.08.001.
13. Weising K., Nybom H., Wolff K., Meyer W. In: DNA Fingerprinting in Plants and Fungi. – USA.: CRC Press, Boca Raton, 1995. – P. 336. ISBN : 0849389208
14. Salybekova N.N., Abdrassulova Zh.T., Kuzhantaeva Zh.Zh., Berdimuratova Zh.B., Babaeva G.A., Serzhanova A.E. Biological features of fungi susceptible varieties Allium Cepa L. // European Conference on Innovations in Technical and Natural Sciences, 6th International scientific conference 4th April, Vienna, Austria. - 2015. - P.17-27.
References
1. Bridge PD, Hawksworth DL, Kozakiewicz Z, Onions AH, Paterson RM, Sackin MJ, Sneath P. H (1989) A reappraisal of the terverticillate penicillia using biochemical, physiological and morphological features I. Numerical taxonomy. Journal of General Microbiology, November. 135: 2941-2966. DOI: 10.1099/00221287-135-11-2941
2. Byther RS, Chastagner GA (1993) Diseases. In: The Physiology of Flower Bulbs, a Comprehensive Treatise on the Physiology and Utilization of Ornamental Flowering Bulbous and Tuberous Plants (Eds.: De Hertogh, A., Le Nard, M.). Elsevier, Amsterdam. pp.71-100.
3. Frisvad JC, Samson RA (2004) Polyphasic taxonomy of Penicillium subgenus Penicillium: a guide to identification of food and air-borne terverticillate Pencillia and their mycotoxins. Stud. Mycol, 49: 1-173.
4. Davis RM (2008) Penicillium decay of garlic. In: Compendium of Onion and Garlic Diseases and Pests (Eds.: Schwartz, H.F., Mohan, S.K.). 2 nd Edn. APS Press, St. Paul, MN. p.52.
5. Sumner DR, Langston DB, Seebold KW (2008) Blue Mold. In: Compendium of Onion and Garlic Diseases and Pests (Eds.: Schwartz, H.F., Mohan, S.K.). 2 nd Edn. APS Press, St. Paul, MN. pp. 52-53.
6. Andersen B, Frisvad JC (2002) Characterization of Alternaria and Penicillium Species from similar substrata based on growth at different temperature, pH and water activity. Syst. Appl. Microbial. 25: 162-172.
7. Nagvi SAMH (2004) Diseases of Fruits and Vegetables: Diagnosis and Management. – USA, Kluwer Academic Publishers, -Р. 537. ISBN 1-4020–1823-1.
8. Bekdairova KZh (1971) Biochemical characterization of garlic and onions in the process of growing and storage, Almaty. Abstract dis. for the degree of candidate of biological sciences. (093) [Biochimicheskaya charakteristica chesnoka i luka v prosesse vegetasii i chranenya] KazSSR. Combine. scientists. Council Institute Comrade soil science, botany, microbiology, virology. – P.28. (In Russian)
9. Burenin VI (1990) Vegetable spring health: [Ovoshi rodnik zdorovia] 3rd ed., Revised. and ext. - L., Lenizdat, -P.255. ISBN 5-289-00741-5. (In Russian)
10. Kalb TJ, Mavlyanova RF (2005) Vegetable production in Central Asia: status and perspectives. Proceedings of the workshop. The World Vegetable Center. Shanhua, Taiwan. AVRDC, - P.134 ISBN 92-9058-139
11. Duduk N, Vasiс M, Vico I. (2014) First Report of Penicillium polonicum Causing Blue Mold on Stored Onion (Allium cepa) in Serbia. Plant Dis., 98 (10): 144-145. DOI:10.1094/PDIS-05-14-0550-PDN.
12. Overy DP, Frisvad JC, Steinmeier U, Thrane U. (2005) Clarification of the agents causing blue mold storage rot uponvarious flower and vegetable bulbs: implicationsfor mycotoxin contamination. Postharvest Biol. and Techn. 35. pp. 217–221. DOI:10.1016/j.postharvbio.2004.08.001.
13. Weising K, Nybom H, Wolff K, Meyer W (1995) In: DNA Fingerprinting in Plants and Fungi. CRC Press, Boca Raton, Florida, USA. – P. 336
14. Salybekova NN, Abdrassulova ZhT, Kuzhantaeva ZhZh, Berdimuratova ZhB, Babaeva GA, Serzhanova AE (2015) Biological features of fungi susceptible varieties Allium сepa L. // European Conference on Innovations in Technical and Natural Sciences 6th International scientific conference 4th April, Vienna, Austria. pp. 17-27.
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Salybekova, N. N., Kuzhantaeva, Z. Z., Basim, E., Serzhanova, A. E., & Seilova, A. E. (2016). Bioecological features of Penicillium aurantiogriseum Dierckx. Experimental Biology, 68(3), 190–196. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/1215
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MICROBIOLOGY
