Evaluation of drought tolerance of soybean on antioxidant enzymes and aldehyde
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Keywords:
drought resistance indices, reactive oxygen species, antioxidant enzymes, aldehyde oxidase.Abstract
The aim of this study was to investigate the expression of antioxidant enzymes - peroxidase (POD) and superoxide dismutase (SOD), aldehyde oxidase (AO) at the level of enzyme activity in order to increase the efficiency of breeding drought-tolerant soybean genotypes. We have studied the cultivated soybean Glycine max L. of the world collection according to the preliminary data having signs of drought resistance: vr. Ustya (Ukraine), K589109 (Russia), K583583 HMAS 84 (USA) and related to early maturing group with vegetation period of 85-95 days. The standard used was variety of domestic breeding - Almaty. Determination of the enzymatic activity of SOD, POD and AO in the leaves of soybean was conducted under irrigation and drought in two reproductive phases of vegetation, the most sensitive to moisture deficit - in the flowering stage R1 and bean filling R5. Screening of the enzymatic activity of SOD, POD and AO showed the distinct correlation links between activity and drought tolerance in Almaty (Kazakhstan) and K583583 (USA) varieties.References
Литература
1. Tardieu F. Plant tolerance to water deficit: physical limits and possibilities for progress // Comp. Rend. Geosci. –2005. –Vol. 337.–P. 57-67.
2. Fehr W.R., Caviness C.E., Burmood O.T. and Pennington J.S. Stage of development description for soybeans Glycine max(L.) // Merril. Crop Sci. – Vol.11. – P. 929-931.
3. Chaves M.M., Oliveira M.M. Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture // J.Exp. Bot. –2004. –V. 55. –No.55. –P.2365-2384.
4. Колупаев Ю.Е., Карпец Ю.В. Формирование адаптивных реакций растений на действие абиотических стрессоров. – Киев: Основа, 2010. – 351 с.
5. Maevskaya S.N., Nikolaeva M.K. Response of Antioxidant and Osmoprotective Systems of Wheat Seedlings to Drought and Rehydration. // Russian Journal of Plant Physiology. – 2013.–V. 60. – No.3. –Р.351–359.
6. Varga B., Janda T., La´szlo´ E., Veisz O. Influence of abiotic stresses on the antioxidant enzyme activity of cereals. // ActaPhysiol Plant. –2012.–Vol.34. –Р.849–858.
7. Helena M. Carvalho C. Drought stress and reactive oxygen species. Production, scavenging and signaling // Plant Signal Behav. – 2008. – Vol. 3. – P.156-165.
8. Suzuki N, Mittler R. Reactive oxygen species and temperature stresses: A delicate balance between signaling and destruction // Physiol. Plant. – 2006. – Vol.. 126. – P. 45-51.
9. Walker-Simmons M., Kudrna D.A., Warner RL. Reduced accumulation of ABA during water stress in a molybdenum cofactor mutant of barley // Plant Physiol. –1988. –Vol. 90. –P. 728-733
10. KoshibaT., SaitoE., OnoN., YamamotoN., SatoM. Purification and properties of flavin and molybdenum-containing aldehyde oxidase from coleoptiles of maize // Plant Physiol. –1996. – Vol.110. –P.781-789.
11. Beauchamp C., Fridovich J. Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels // Anal. Biochem. –1971.–Vol. 44. –P. 276-287.
12. Лебедева О.В., Угарова Н.Н., Березин И.В. Кинетическое изучение реакции окисления о-дианизидина Н2О2 в присутствии пероксидазы хрена // Биохимия. –1977.–Т.42. – С.1372-1379.
13. Rothe G.M. Aldehyde oxidase isoenzymes (EC 1.2.3.1) in potato tubers (Solanumtuberosum). //Plant Cell Physiol. –1974.–Vol.15.–Р.493-499.
14. Laemmli K. Cleavage of structural proteins during the assembly of the headof bacteriophage // Nature. –1970. –Vol.4. – №227. –P.680-685.
15. Oliver H. Lowry, Nira J. Rosebrough, A.Lewis Farr and Rose J. Randall Protein measurement with the folin phenol reagent // J. Biol. Chem. –1951. –Vol.193. – P. 265-275.
References
1. Tardieu F. Plant tolerance to water deficit: physical limits and possibilities for progress // Comp. Rend. Geosci. –2005. –Vol. 337.–P. 57-67.
2. Fehr W.R., Caviness C.E., Burmood O.T. and Pennington J.S. Stage of development description for soybeans Glycine max(L.) // Merril. Crop Sci. – Vol.11. – P. 929-931.
3. Chaves M.M., Oliveira M.M. Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture // J.Exp. Bot. –2004. –V. 55. –No.55. –P.2365-2384.
4. Kolupaev Y.E., Karpets Y.V. Formation of adaptive responses of plants to abiotic stressors action. –Kiev: Osnova, 2010. – 351 p.
5. Maevskaya S.N., Nikolaeva M.K. Response of Antioxidant and Osmoprotective Systems of Wheat Seedlings to Drought and Rehydration. // Russian Journal of Plant Physiology. – 2013.–V. 60. – No.3. –Р.351–359.
6. Varga B., Janda T., La´szlo´ E., Veisz O. Influence of abiotic stresses on the antioxidant enzyme activity of cereals. // ActaPhysiol Plant. –2012.–Vol.34. –Р.849–858.
7. Helena M. Carvalho C. Drought stress and reactive oxygen species. Production, scavenging and signaling // Plant Signal Behav. – 2008. – Vol. 3. – P.156-165.
8. Suzuki N, Mittler R. Reactive oxygen species and temperature stresses: A delicate balance between signaling and destruction // Physiol. Plant. – 2006. – Vol. 126. – P. 45-51.
9. Walker-Simmons M., Kudrna D.A., Warner RL. Reduced accumulation of ABA during water stress in a molybdenum cofactor mutant of barley // Plant Physiol. –1988. – Vol. 90. –P. 728-733
10. KoshibaT., SaitoE., OnoN., YamamotoN., SatoM. Purification and properties of flavin and molybdenum-containing aldehyde oxidase from coleoptiles of maize // Plant Physiol. –1996. – Vol.110. –P.781-789.
11. Beauchamp C., Fridovich J. Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels // Anal. Biochem. –1971.–Vol. 44. –P. 276-287.
12. Lebedev O.V., Ugarov N.N., Berezin I.V. Kinetic study of the oxidation reaction of o-dianisidine in the presence of H2O2 horseradish peroxidase // Biochemistry. – 1977. – Vol.42.– P.1372-1379.
13. Rothe G.M. Aldehyde oxidase isoenzymes (EC 1.2.3.1) in potato tubers (Solanumtuberosum). //Plant Cell Physiol. –1974.–Vol.15.–Р.493-499.
14. Laemmli K. Cleavage of structural proteins during the assembly of the headof bacteriophage // Nature. –1970. –Vol..4. – №227. –P.680-685.
15. Oliver H. Lowry, Nira J. Rosebrough, A.Lewis Farr and Rose J. Randall Protein measurement with the folin phenol reagent // J. Biol. Chem. –1951. –Vol.193. – P. 265-275.
1. Tardieu F. Plant tolerance to water deficit: physical limits and possibilities for progress // Comp. Rend. Geosci. –2005. –Vol. 337.–P. 57-67.
2. Fehr W.R., Caviness C.E., Burmood O.T. and Pennington J.S. Stage of development description for soybeans Glycine max(L.) // Merril. Crop Sci. – Vol.11. – P. 929-931.
3. Chaves M.M., Oliveira M.M. Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture // J.Exp. Bot. –2004. –V. 55. –No.55. –P.2365-2384.
4. Колупаев Ю.Е., Карпец Ю.В. Формирование адаптивных реакций растений на действие абиотических стрессоров. – Киев: Основа, 2010. – 351 с.
5. Maevskaya S.N., Nikolaeva M.K. Response of Antioxidant and Osmoprotective Systems of Wheat Seedlings to Drought and Rehydration. // Russian Journal of Plant Physiology. – 2013.–V. 60. – No.3. –Р.351–359.
6. Varga B., Janda T., La´szlo´ E., Veisz O. Influence of abiotic stresses on the antioxidant enzyme activity of cereals. // ActaPhysiol Plant. –2012.–Vol.34. –Р.849–858.
7. Helena M. Carvalho C. Drought stress and reactive oxygen species. Production, scavenging and signaling // Plant Signal Behav. – 2008. – Vol. 3. – P.156-165.
8. Suzuki N, Mittler R. Reactive oxygen species and temperature stresses: A delicate balance between signaling and destruction // Physiol. Plant. – 2006. – Vol.. 126. – P. 45-51.
9. Walker-Simmons M., Kudrna D.A., Warner RL. Reduced accumulation of ABA during water stress in a molybdenum cofactor mutant of barley // Plant Physiol. –1988. –Vol. 90. –P. 728-733
10. KoshibaT., SaitoE., OnoN., YamamotoN., SatoM. Purification and properties of flavin and molybdenum-containing aldehyde oxidase from coleoptiles of maize // Plant Physiol. –1996. – Vol.110. –P.781-789.
11. Beauchamp C., Fridovich J. Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels // Anal. Biochem. –1971.–Vol. 44. –P. 276-287.
12. Лебедева О.В., Угарова Н.Н., Березин И.В. Кинетическое изучение реакции окисления о-дианизидина Н2О2 в присутствии пероксидазы хрена // Биохимия. –1977.–Т.42. – С.1372-1379.
13. Rothe G.M. Aldehyde oxidase isoenzymes (EC 1.2.3.1) in potato tubers (Solanumtuberosum). //Plant Cell Physiol. –1974.–Vol.15.–Р.493-499.
14. Laemmli K. Cleavage of structural proteins during the assembly of the headof bacteriophage // Nature. –1970. –Vol.4. – №227. –P.680-685.
15. Oliver H. Lowry, Nira J. Rosebrough, A.Lewis Farr and Rose J. Randall Protein measurement with the folin phenol reagent // J. Biol. Chem. –1951. –Vol.193. – P. 265-275.
References
1. Tardieu F. Plant tolerance to water deficit: physical limits and possibilities for progress // Comp. Rend. Geosci. –2005. –Vol. 337.–P. 57-67.
2. Fehr W.R., Caviness C.E., Burmood O.T. and Pennington J.S. Stage of development description for soybeans Glycine max(L.) // Merril. Crop Sci. – Vol.11. – P. 929-931.
3. Chaves M.M., Oliveira M.M. Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture // J.Exp. Bot. –2004. –V. 55. –No.55. –P.2365-2384.
4. Kolupaev Y.E., Karpets Y.V. Formation of adaptive responses of plants to abiotic stressors action. –Kiev: Osnova, 2010. – 351 p.
5. Maevskaya S.N., Nikolaeva M.K. Response of Antioxidant and Osmoprotective Systems of Wheat Seedlings to Drought and Rehydration. // Russian Journal of Plant Physiology. – 2013.–V. 60. – No.3. –Р.351–359.
6. Varga B., Janda T., La´szlo´ E., Veisz O. Influence of abiotic stresses on the antioxidant enzyme activity of cereals. // ActaPhysiol Plant. –2012.–Vol.34. –Р.849–858.
7. Helena M. Carvalho C. Drought stress and reactive oxygen species. Production, scavenging and signaling // Plant Signal Behav. – 2008. – Vol. 3. – P.156-165.
8. Suzuki N, Mittler R. Reactive oxygen species and temperature stresses: A delicate balance between signaling and destruction // Physiol. Plant. – 2006. – Vol. 126. – P. 45-51.
9. Walker-Simmons M., Kudrna D.A., Warner RL. Reduced accumulation of ABA during water stress in a molybdenum cofactor mutant of barley // Plant Physiol. –1988. – Vol. 90. –P. 728-733
10. KoshibaT., SaitoE., OnoN., YamamotoN., SatoM. Purification and properties of flavin and molybdenum-containing aldehyde oxidase from coleoptiles of maize // Plant Physiol. –1996. – Vol.110. –P.781-789.
11. Beauchamp C., Fridovich J. Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels // Anal. Biochem. –1971.–Vol. 44. –P. 276-287.
12. Lebedev O.V., Ugarov N.N., Berezin I.V. Kinetic study of the oxidation reaction of o-dianisidine in the presence of H2O2 horseradish peroxidase // Biochemistry. – 1977. – Vol.42.– P.1372-1379.
13. Rothe G.M. Aldehyde oxidase isoenzymes (EC 1.2.3.1) in potato tubers (Solanumtuberosum). //Plant Cell Physiol. –1974.–Vol.15.–Р.493-499.
14. Laemmli K. Cleavage of structural proteins during the assembly of the headof bacteriophage // Nature. –1970. –Vol..4. – №227. –P.680-685.
15. Oliver H. Lowry, Nira J. Rosebrough, A.Lewis Farr and Rose J. Randall Protein measurement with the folin phenol reagent // J. Biol. Chem. –1951. –Vol.193. – P. 265-275.
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Spankulova, Z. B., Li, T. E., Didorenko, S. V., & Orazbaеva U. M. (2016). Evaluation of drought tolerance of soybean on antioxidant enzymes and aldehyde. Experimental Biology, 65(3), 298–303. Retrieved from https://bb.kaznu.kz/index.php/biology/article/view/1131
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
