POLYMORPHISM of NUCLEOTIDE SEQUENCES of GENES THAT DETERMINE the VIRULENCE of ENTOMOPATHOGENIC FUNGUS Beauveria bassiana
Keywords:
Beauveria bassiana, genetic polymorphism, molecular marker, excreted lipase.Abstract
It has been selected primers used for genotyping of three closely related Beauveria bassiana strains of different origin based on the analysis of nucleotide sequences of the inducible genes of the fungus that are involved in the pathogenesis of fungal infections of insects. Most of the sequences of genes of the three strains had homology at the level of 98-100 %, but the phase sequence of the gene encoding the excreted lipase (Slip), were significantly more polymorphic (homology 89-90 %). The use of this locus as a biogeographic molecular marker allowed to clearly differentiate strains of differing origin.References
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2. Gibson D.M., Donzelli B.G., Krasnoff S.B., Keyhani N.O. (2014) Discovering the secondary metabolite potential encoded within entomopathogen-ic fungi. Nat Prod Rep 31:1287–1305
3. Ortiz-Urquiza A., Keyhani N.O. (2013) Action on the surface: Entomopathogenic fungi versus the insect cuticle. Insects 4:357–374
4. Ortiz-Urquiza A., Luo Zh., Keyhani N. O. (2015) Improving mycoinsecticides for insect biological control. Appl Microbiol Biotechnol 99(3):1057-68
5. Pedrini N., Ortiz-Urquiza A., Huarte-Bonnet C., Zhang S., Keyhani N.O. (2013) Targeting of insect epicuticular lipids by the entomopathogenic fungus Beauveria bassiana: hydrocarbon oxidation within the context of a host-pathogen interaction. Front Microbiol 4:24
6. Xiao G., Ying SH., Zheng P., Wang Z.L., Zhang S., Xie X.Q., Shang Y, St. Leger R.J., Zhao G.P., Wang C., Feng M.G. (2012) Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana. Sci Rep 2:483
7. Zhang S., Widemann E., Bernard G., Lesot A., Pinot F., Pedrini N., Keyhani N.O. (2012) CYP52X1, representing new cytochrome P450 subfamily, displays fatty acid hydroxylase activity and contributes to virulence and growth on insect cuticular substrates in entomopathogenic fungus Beauveria bassiana. J Biol Chem 287:13477–13486.
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