Особенности сайтов связывания miR-574-5р и miR-574-3р с mRNA генов-мишеней

S. A. Atambayeva; R. Y. Niyazova; O. A. Berillo; A. T. Ivashchenko

Authors

S. A. Atambayeva Национальная нанотехнологическая лаборатория КазНУ им. аль-Фараби, Республика Казахстан, г. Алматы
R. Y. Niyazova Национальная нанотехнологическая лаборатория КазНУ им. аль-Фараби, Республика Казахстан, г. Алматы
O. A. Berillo Национальная нанотехнологическая лаборатория КазНУ им. аль-Фараби, Республика Казахстан, г. Алматы
A. T. Ivashchenko Национальная нанотехнологическая лаборатория КазНУ им. аль-Фараби, Республика Казахстан, г. Алматы

Keywords:

miRNA, mRNA, miR-574-5p, miR-574-3p, binding sites, target genes.

Abstract

The binding of miR-574-5p and miR-574-3p with their target genes mRNAs was studied. miR-574-5p binding sites are located in the 3'UTR of 244 genes, in the 5'UTR of 20 genes and in the CDS of two genes. miR-574-5p binding sites in the CDS of REM2 and FGFRL1 genes with the high ΔG/ΔGm value encode oligopeptides HTHTHTHS and DTDMDTDT in the corresponding proteins. miR-574-3p binding sites are located in the 3'UTR of genes GRK6, NACC2, SEC14L1 and in the 5'UTR of the gene ZNF470. In the majority of miR-574-5p target genes mRNAs there are multiple binding sites with the ΔG/ΔGm value greater than 90%, which arranged located through two nucleotides. Changes in the miR-574-5p and miR-574-3p expression at many pathologies, including cancer, correlated with changes in the expression of their target genes.