Оптимизация условий Agrobacterium – опосредованной трансформации хлопчатника. Мақтаның Agrobacterium арқылы трансформациялау шарттарын оңтайландыру
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Ключевые слова:
хлопчатник, Agrobacterium–опосредованная трансформация, мақта, Agrobacterium – арқылы трансформация,Аннотация
В статье приведены данные по оптимизации условий Agrobacterium–опосредованной трансформации хлопчатника сортов отечественной селекции. Выявлена оптимальная концентрация ацетосирингон (100 мкМ), оптическая плотность суспензии бактерий (0,6) и время ко-культивации (48 часов) со штаммом A.tumefaciens LBA4404 для Agrobacterium–опосредованной трансформации эксплантов хлопчатника. Полученные результаты закладывают базу для создания трансгенных растений отечественных сортов хлопчатника, устойчивых к гербициду Баста. Мақалада мақтаның отандық селекция сұрыптарын Agrobacterium арқылы трансформациялаудың шарттарын оңтайландыру жөнінен мәліметтер келтірілген. Мақта экспланттарын Agrobacterium арқылы трансформациялау үшін ацетосирингонның (100мкМ) оңтайлы концентрациясы, бактерия суспензиясының оптикалық тығыздығы (0,6) мен Agrobacterium tumefaciens LBA4404 штамымен кокультивациялау уақыты (48 сағат) анықталды. Алынған нәтижелер Баста гербицидіне төзімді отандық мақта сұрыптарының трансгенді өсімдіктерін алу үшін негіз қалайды.Библиографические ссылки
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3 Brimner T.A., Gallivan G.J., Stephenson G.R. Influence of herbicide-resistant canola on the environmental impact of weed management // Pest Management Science. – 2005. – Vol. 61. – P. 47-52.
4 Murashige T., Skoog F. A revised medium for rapid growth and biosynthesis with tobacco tissue culture //Plant Physiology.- 1962. – Vol. 15. – P. 473-497.
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6 Sunilkumar G., Rathore K. S. Transgenic cotton: factors influencing Agrobacterium-mediated transformation and regeneration // Molecular Breeding. – 2001. – Vol. 8. –P. 37–52.
7 Zapata C., Park S.H., El-Zik K.M. and Smith R.H. Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex //Theoretical and Applied Genetics. – 1999.- Vol. 98.– P. 252–256.
8 Khan T., Reddy V. S., Leelavathi S. High-frequency regeneration via somatic embryogenesis of an elite recalcitrant cotton genotype (GossypiumhirsutumL.) and efficient Agrobacterium-mediated transformation // Plant Cell Tissue and Organ Culture. – 2010. –Vol. 101. – P. 323-330.
9 Khan T., Reddy V. S., Leelavathi S. High-frequency regeneration via somatic embryogenesis of an elite recalcitrant cotton genotype (Gossypium hirsutum L.) and efficient Agrobacterium-mediated transformation // Plant Cell Tissue and Organ Culture. – 2010. –Vol. 101. – P. 323-330.
10 Rashid H., Yokoi S., Toriyama K. and Hinata K. Trans-genic plant production mediated by Agrobacterium in Indicarice //Plant Cell Reports. – 1996. – Vol. 15. – P. 727–730.
2 Herouet C., Esdaile D.J., Mallyon B.A. et al. Safety evaluation of the phosphinothricinacetyltransferase proteins encoded by the pat and bar sequences that confer tolerance to glufosinate-ammonium herbicide in transgenic plants // Regulatory Toxicology and Pharmacology. – 2005. – Vol. 41. – P. 134–149.
3 Brimner T.A., Gallivan G.J., Stephenson G.R. Influence of herbicide-resistant canola on the environmental impact of weed management // Pest Management Science. – 2005. – Vol. 61. – P. 47-52.
4 Murashige T., Skoog F. A revised medium for rapid growth and biosynthesis with tobacco tissue culture //Plant Physiology.- 1962. – Vol. 15. – P. 473-497.
5 Jefferson R. A., Kavanagh T.A., Bevan M. W. GUS fusions: β-B-glucuronidase as a sensitive and versatile gene fusion marker in higher plants // The EMBO Journal. – 1987. – Vol. 6. – P.3901 -3907.
6 Sunilkumar G., Rathore K. S. Transgenic cotton: factors influencing Agrobacterium-mediated transformation and regeneration // Molecular Breeding. – 2001. – Vol. 8. –P. 37–52.
7 Zapata C., Park S.H., El-Zik K.M. and Smith R.H. Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex //Theoretical and Applied Genetics. – 1999.- Vol. 98.– P. 252–256.
8 Khan T., Reddy V. S., Leelavathi S. High-frequency regeneration via somatic embryogenesis of an elite recalcitrant cotton genotype (GossypiumhirsutumL.) and efficient Agrobacterium-mediated transformation // Plant Cell Tissue and Organ Culture. – 2010. –Vol. 101. – P. 323-330.
9 Khan T., Reddy V. S., Leelavathi S. High-frequency regeneration via somatic embryogenesis of an elite recalcitrant cotton genotype (Gossypium hirsutum L.) and efficient Agrobacterium-mediated transformation // Plant Cell Tissue and Organ Culture. – 2010. –Vol. 101. – P. 323-330.
10 Rashid H., Yokoi S., Toriyama K. and Hinata K. Trans-genic plant production mediated by Agrobacterium in Indicarice //Plant Cell Reports. – 1996. – Vol. 15. – P. 727–730.
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Manabayeva., S. A., & Rahimzhanova, A. O. (2015). Оптимизация условий Agrobacterium – опосредованной трансформации хлопчатника. Мақтаның Agrobacterium арқылы трансформациялау шарттарын оңтайландыру. Вестник КазНУ. Серия биологическая, 60(1), 200–203. извлечено от https://bb.kaznu.kz/index.php/biology/article/view/94
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