Разработка альтернативного метода исследования местнораздражающего действия координационного соединения иода in vitro
DOI:
https://doi.org/10.26577/eb-2018-2-1335Аннотация
Развитие альтернативных методов в токсикологическом тестировании является актуальным по нескольким причинам: а) возможность протестировать большое количество веществ за сравнительно небольшой отрезок времени и в одинаковых условиях; б) наличие разнообразных моделей in vitro; в) снижение стоимости исследований за счёт замены лабораторных животных моделями in vitro. Одним из результатов этой работы становится гуманизация токсикологических исследований через уменьшение количества лабораторных животных, используемых в эксперименте. В связи с этим разработка метода оценки местнораздражающего действия некоторых веществ, обладающих заведомо коррозийным действием на слизистые оболочки, будет полезной, особенно для таких веществ, как иод и его комплексы c биоорганическими лигандами.
Целью данной работы являлась оценка возможности использования клеточной линии MDCK в качестве in vitro модели для изучения раздражающего действия новых координационных соединений иода (КС-143, КС-144, КС-145) с использованием МТТ-теста. Референсным веществом было лекарственное средство иод повидон (ПВП-иод), натриевая соль лаурилсерной кислоты (ЛСН) использовалась как хорошо изученное и рекомендованное для токсикологических исследований in vitro контрольное вещество.
В данной работе было показано, что ПВП-иод, ЛСН, КС-143, КС-144 и КС-145 показывают классическую дозозависимую цитотоксичность в отношении клеток MDCK. Цитотоксическая концентрация (ЦТК50) для ПВП-иода достоверно ниже по сравнению с ЦТК50 для КС. При этом цитотоксическое действие КС на MDCK не зависит от времени инкубации, от 3 до 72 часов.
Практическое значение работы заключается в том, что клеточную линию MDCK можно предложить в качестве альтернативной модели в скрининговых исследованиях местно- раздражающего действия новых КС соединений иода.
Ключевые слова: альтернативный метод, MDCK, модель эпителиальных клеток in vitro, координационное соединение иода.
Библиографические ссылки
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Majda А., Chuscielska K. Estimation of damaging effects of chemicals on the mucous membrane rabbit eyes �� Medicine Pracy, XXIV, − 1973. – Vol. 3. – P. 321-336.
EЭС 76�768�ЕЕС (Cosmetics Directive) Directive 2003�15�EC of 27 February 2003 is the 7th amendment to the law.
Завьялов Н.В., Червонская Г.П., Панкратова Г.П. и др. Ускоренное изучение цитотоксического действия в экспресс- тестах in vitro �� Сб. тезисов докл. 1-го съезда токсикологов. – 1998. – Москва. – С. 279.
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Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays ��
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EЭС 76�768�ЕЕС (Cosmetics Directive) Directive 2003�15�EC of 27 February 2003 is the 7th amendment to the law.
Завьялов Н.В., Червонская Г.П., Панкратова Г.П. и др. Ускоренное изучение цитотоксического действия в экспресс- тестах in vitro �� Сб. тезисов докл. 1-го съезда токсикологов. – 1998. – Москва. – С. 279.
Russell W.M.S., Burch R.�. The Principles of Humane Experimental Techniques �� Methuen and Co., �ondon, UK, − 1950. – P. 238.
Ekwall B., Clemedson C., Ekwall B. [et al.] EDIT: a new international multicentre programme to develop and evaluate batteries of in vitro tests for acute and chronic systemic toxicity �� − 1999. – Vol. 27. – P. 339-349.
Трахтенберг І.М., Коваленко В.М., Кокшарьова Н.В., Жмінько П.Г. Альтернативні методи і тест-системи. Лікарська токсикологія �� АМН України І.М. Трахтенберга. – 2008. – C. 272.
Wilson S.�., Ahearne, M., Hopkinson, A. An overview of current techniques for ocular toxicity testing. �� Toxicology 327. − 2015. – P. 32–46.
Hoffmann S., Hartung T. Designing validation studies more efficiently according to the modular approach: Retrospective analy- sis of the EPISKIN test for skin corrosion �� Altern �ab Anim 34. – 2006. – P. 177-191.
Contini S., Scarpignato C. Caustic injury of the upper gastrointestinal tract: A comprehensive review �� World �ournal of Gas- troenterology. – 2013. – Vol. 19, No 25. – P. 3918-3930.
Gordon S., Daneshian M., Bouwstra �., [et al.] Non-Animal Models of Epithelial Barriers (Skin, Intestine and �ung) in Research
�� Industrial Applications and Regulatory Toxicology. – 2015. – Altex 32(4). – P. 341.
Tsurumaru D., Utsunomiya T., Matsuura S., Komori M., Kawanami S., Ishibashi T., Honda H. Gastric mucosal changes caused by �ugol’s iodine solution spray: endoscopic features of 64 cases on screening esophagogastroduodenoscopy �� Gastroenterology Research and Practice. − 2010. – Vol. 2010. – P. 1-4.
Kanagalingam �., Chopra A., Hong M.H., Ibrahim W., Villalon A., �in �-C. Povidone-iodine for the management of oral muco- sitis during cancer therapy �� �ncology Reviews. – 2017. – Vol. 11(2). – P.341.
Ilin A.I., Kulmanov M.E. Antibacterial agent for treating infectious diseases of bacterial origin �� − 2014. – Patent 389 US 2014�0010782 A1
�uldasheva G.A., Zhidomirov G.M., Abekova A.�., Ilin A.I. The Mechanism of Anti-Cancer Activity of Complexes of Molec- ular Iodine with α-Dextrins and Polypeptides and �ithium Halogenides �� �ournal of Antivirals and Antiretrovirals. – 2016. – Vol. 8.
– P. 072-078. D�I:10.4172�jaa.1000138.
Madin S.H., Darby N.B. U.S. Dept. HEW, P.H.S. Registry of Animal Cell �ines �� Proc Soc Exp Biol Med. − 1965. – Vol. 98.
– P. 574-576.
Gaush C.R., Hard W.�., Smith T.F. Characterization of an established line of canine kidney cells (MDCK), Proc Soc Exp �� Biol Med. – 1966. – Vol. 122. – P. 931-935.
Taub M., Ue B., Chuman �., Rindler M.�., Saier M.H. Alterations in growth requirements of kidney epithelial cells in defined medium associated with malignant transformation. �� Methods Enzymol. – 1979. – Vol. 58. – P. 552-560.
Taub M., Ue B., Chuman �., Rindler M.�., Saier M.H. Supramolecule Structure �� − 1981. – Vol. 15. – P. 63-72.
�ECD Guideline for the Testing of Chemicals Section 4: Health Effects. Test No. 431: In Vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test Method. – 2013. – D�I: 10.1787�9789264203822-en.
Fukuda M., Murano H., �amashiro �., Takahashi N. �ournal Investigative �phthalmology & Visual Science: Effects of Povi- done Iodine on Cultured Corneal Epithelial Cells �� ARV� Annual Meeting Abstract. – 2003. – Vol. 44, Issue 13. – P. 66.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays ��
�ournal of Immunological Methods. – 1983. – Vol. 65, No 1-2. – P. 55-63.
Wutzler P., Sauerbrei A., Klöcking R., Brögmann B., Reimer K. Virucidal activity and cytotoxicity of the liposomal formulation of povidone-iodine �� Antiviral Res. – 2002. – Vol. 54, No 2. – P. 89-97.
Noda �., Fujii K., Fujii S. Critical evaluation of cadexomer-iodine ointment and povidone-iodine sugar ointment �� Int � Pharm.
– 2009. – Vol. 8, No. 372(1-2). – P.85-90.
Müller G., Kramer A. Biocompatibility index of antiseptic agents by parallel assessment of antimicrobial activity and cellular cytotoxicity �� � Antimicrob Chemother. – 2008. – Vol. 61, No 6. – P. 1281-1287.
Doan �., Piskoun B., Rosenberg A.D., Blanck T.�., Phillips M.S., Xu F. In vitro antiseptic effects on viability of neuronal and Schwann cells �� Reg Anesth Pain Med. – 2012. – Vol. 37, No 2. – P. 131-138.
Rusanov A.�., �uzgina N.G., �isitsa A.V. Sodium dodecyl sulfate cytotoxicity towards HaCaT keratinocytes: comparative analysis of methods for evaluation of cell viability �� Bull Exp Biol Med. – 2017. – Vol. 163, No 2. – P. 284-288.
Kataoka M., Tsumura H., Kaku N., Torisu T. Toxic effects of povidone-iodine on synovial cell and articular cartilage �� Clin Rheumatol. – 2006. – Vol. 25, No 5. – P.632-638.
References
Cinelli S., Falezza A., Meli C. [et al.] (1991) Alternative methods in toxicology tests: in vitro toxicity. Cytotechnology., vol. 5, no. 1, pp. 51-54 (European Commision-Enterprise and Industry-REACH – �verview-FAG. Web page: http:��eu.europa.eu�enter- prise�reach�fag_en.htm.
Contini S., Scarpignato C. (2013) Caustic injury of the upper gastrointestinal tract: A comprehensive review., World �ournal of Gastroenterology., vol. 19, no. 25, pp. 3918-3930.
Doan �., Piskoun B., Rosenberg A.D., Blanck T.�., Phillips M.S., Xu F. (2012) In vitro antiseptic effects on viability of neuronal and Schwann cells. Reg Anesth Pain Med., vol. 37, no. 2, pp. 131-138.
Ekwall B., Clemedson C., Ekwall B. [et al.] (1999) EDIT: a new international multicentre programme to develop and evaluate batteries of in vitro tests for acute and chronic systemic toxicity., vol. 27, pp. 339-349.
EЭС 76�768�ЕЕС (Cosmetics Directive) Directive 2003�15�EC of 27 February 2003 is the 7th amendment to the law.
Fukuda M., Murano H., �amashiro �., Takahashi N. (2003) Effects of Povidone Iodine on Cultured Corneal Epithelial Cells.
�ournal Investigative �phthalmology & Visual Science., ARV� Annual Meeting Abstract., vol. 44, Issue 13, pp. 66.
Gaush C.R., Hard W.�., Smith T.F. (1966) Characterization of an established line of canine kidney cells (MDCK), Proc Soc Exp. Biol Med., vol. 122, pp. 931-935.
Gordon S., Daneshian M., Bouwstra �., [et al.] (2015) Non-Animal Models of Epithelial Barriers (Skin, Intestine and �ung) in Research. Industrial Applications and Regulatory Toxicology., Altex 32(4), p. 341.
Hoffmann S., Hartung T. (2006) Designing validation studies more efficiently according to the modular approach: Retrospective analysis of the EPISKIN test for skin corrosion. Altern �ab Anim 34., pp. 177-191.
Ilin A.I., Kulmanov M.E. (2014) Antibacterial agent for treating infectious diseases of bacterial origin. Patent 389 US 2014�0010782 A1
Kanagalingam �., Chopra A., Hong M.H., Ibrahim W., Villalon A., �in �-C. (2017) Povidone-iodine for the management of oral mucositis during cancer therapy., �ncology Reviews., vol. 11(2), p.341.
Kataoka M., Tsumura H., Kaku N., Torisu T. (2006) Toxic effects of povidone-iodine on synovial cell and articular cartilage.
Clin Rheumatol., vol. 25, no. 5, pp.632-638.
Madin S.H., Darby N.B. (1965) U.S. Dept. HEW, P.H.S. Registry of Animal Cell �ines. Proc Soc Exp Biol Med., vol. 98, pp. 574-576.
Majda А., Chuscielska K. (1973) Estimation of damaging effects of chemicals on the mucous membrane rabbit eyes., Medicine Pracy, XXIV, , vol.3, pp. 321-336
Mosmann T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity as- says. �ournal of Immunological Methods., vol. 65, no. 1-2, pp. 55-63.
Müller G., Kramer A. (2008) Biocompatibility index of antiseptic agents by parallel assessment of antimicrobial activity and cellular cytotoxicity. � Antimicrob Chemother., vol. 61, no. 6, pp. 1281-1287.
Noda �., Fujii K., Fujii S. (2009) Critical evaluation of cadexomer-iodine ointment and povidone-iodine sugar ointment. Int � Pharm., vol. 8, no. 372 (1-2), pp.85-90.
�ECD Guideline for the Testing of Chemicals (2013) Section 4: Health Effects. Test No. 431: In Vitro Skin Corrosion: Recon- structed Human Epidermis (RHE) Test Method. D�I: 10.1787�9789264203822-en.
Rusanov A.�., �uzgina N.G., �isitsa A.V. (2017) Sodium dodecyl sulfate cytotoxicity towards HaCaT keratinocytes: compara- tive analysis of methods for evaluation of cell viability. Bull Exp Biol Med., vol. 163, no. 2, pp. 284-288.
Russell W.M.S., Burch R.�. (1950) The Principles of Humane Experimental Techniques. Methuen and Co., �ondon, UK, pp.
238.
Taub M., Ue B., Chuman �., Rindler M.�., Saier M.H. (1981) Supramolecule Structure., vol. 15, pp. 63-72.
Taub M., Ue B., Chuman �., Rindler M.�., Saier M.H. (1979) Alterations in growth requirements of kidney epithelial cells in
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Как цитировать
Abekova А. О., Kenzhebekova R. Т., Abramova, Z. S., Islamov R. А., Nersesyan, A. K., & Ilin А. I. (2018). Разработка альтернативного метода исследования местнораздражающего действия координационного соединения иода in vitro. Вестник КазНУ. Серия биологическая, 75(2), 134–142. https://doi.org/10.26577/eb-2018-2-1335
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Раздел
ФИЗИОЛОГИЯ И БИОХИМИЯ ЧЕЛОВЕКА И ЖИВОТНЫХ
