Obtaining Mutant Strain of Chlamydomonas reinhardtii and Studying Its Phenotypic Characteristics.
Keywords:
UV mutagenesis, mutant strain Chlamydomonas reinhardtii CC 1021 Mut1, Phenotype (light green color and small size colonies),Abstract
UV mutagenesis is a powerful tool for obtaining mutant strains of microalgae for using in biotesting of the polluted aquatic ecosystems. UV irradiation has a strong mutagenic agent, compared with chemical mutagenesis, UV mutagenesis offers many advantages such as less pollution, simple operation, and sterile cultivation condition. In this study the phenotypic characteristics of the wild and mutant strains of the green microalgae Chlamydomonas reinhardtii (CC 1021) were investigated. From the obtained results, selection of the mutant colony after irradiation time 1min. and named CC1021Mut1 strain. The mutant strain showed differences in phenotypic features such as color and size of colonies in the solid medium and also different color in the liquid medium. The mutant cells under microscope appeared different in size and color than the wildtype cells.References
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2 Holzinger A., Lütz C., Algae and UV irradiation: effects on ultrastructure and related metabolic functions. Micron
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3 Charles D.A. Algal Chemical Ecology // ch. 13, Karsten U. - Springer-Verlag Berlin Heidelberg, 2008. – 376 p.
4 Karentz D., Cleaver J.E., Mitchell D.L. Cell survival characteristics and molecular responses of Antarctic phytoplankton to ultraviolet-B radiation // J. Phycol. – 1991. – V. 27. – P. 326–341.
5 Hercegová A., Ševčovičová A., Gálová E., UV light-induced DNA damage detection in the unicellular green alga
Chlamydomonas reinhardtii // Biologia. – 2008. – V.63 (6). – P. 958-961.
6 Harris E.H. The Chlamydomonas Sourcebook: Introduction in Chlamydomonas and its Laboratory Use. 2nd. Ed.Elsevier Inc., 2009. – 183 p.
7 Sager R., Palade G.E. Chloroplast structure in green and yellow strains of Chlamydomonas // Exp. Cell Res. – 1954.
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8 Harris EH The Chlamydomonas Sourcebook: A Comprehensive Guide to Biology and Laboratory Use. – Academic Press, San Diego, CA., 1989. – 294 p.
9 Zhang Y.Q., Lu D.X, Wang Y.J. Effects of ultraviolet radiation on the proein contents of three marine microalgae
species. J. Hui Agricult. Sci. - 2009. – V. 37 – P. 9350-9351.
10 Hagen C, Braune W, Greulich F. Functional aspects of secondary carotenoids in Heamatococcus lacustris (Girod)
Rostafinski (Volvocales) IV, Protection from photodynamic damage // J. Photo chem.. Photobiol. B: Bio. – 1993. – V.
20. – P. 153-160.
11 Andrea L., Leland S. Contrasting Effects of UV-A and UV-B on Photosynthesis and Photoprotection of β-carotene
in two Dunaliella spp. // Plant Cell Physiol. – 2002. – V. 43 (8). –P. 877-884. doi: 10.1093/pcp/pcf105.
12 Solovchenko A, Merzlyak M. Screening of visible and UV radiation as a photoprotective mechanism in plants //
Russian J. of Plant Physiology. – 2008. – V. 55(6). – P. 719-737.
13 Demmig-Adams B., Gilmore A.M., Adams W.W. III In vivo functions of carotenoids in higher plants // FASEB
Journal. – 1996. – V. 10. – P. 403–412.
14 Xiong F.S., Day T.A. Effect of solar ultraviolet-B radiation during springtime ozone depletion on photosynthesis and biomass production of Antarctic vascular plants // Plant Physiology. – 2001. – V. 125. – P. 738–751.
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