BIOTESTING OF ENTOMOPATHOGENIC FUNGI BEAUVERIA BASSIANA AGAINST THE WHITEFLY (TRIALEURODES VAPORARIORUM): LABORATORY AND FIELD EXPERIMENTS

Authors

  • Т. Doolotkeldieva Kyrgyz-Turkish Manas University, Kyrgyzstan, Bishkek
  • S. Zholdoshbekova, Kyrgyz-Turkish Manas University, Kyrgyzstan, Bishkek
  • E. Ismailova Kyrgyz-Turkish Manas University, Kyrgyzstan, Bishkek

DOI:

https://doi.org/10.26577/eb.2022.v90.i1.05
        52 124

Abstract

In the present study, we searched for the composition of cheap nutrient media. We grew local isolates of Beauveria bassiana (Ascomycota: Clavicipitaceae) isolated from various natural sources in Kyrgyzstan. To produce biomass from strains of Beauveria bassiana in laboratory conditions and determine its entomopathogenic activity, food industry wastes were used: pea groats, oatmeal and soy flour. Strains 501Bb, 502Bb and 503Bb gave a tremendous growth of mycelium and conidia formation on the surface of oatmeal and pea groats when counting the resulting conidia in 1g of the biomass, the density of conidia was 1.2-1.4 x 109 spores/ml within 15 days. Weak growth was noted on soy flour; conidia was 1.0x104 spores/ml.

 

References

Aung O. M., Soytong K., Hyde K. D. Diversity of entomopathogenic fungi in rainforests of Chiang Mai Province, Thailand

//Fungal Diversity. – 2008. – Vol. 30. – P. 15–22.

Boucias D. G. Microbiota in insect fungal pathology //Applied microbiology and biotechnology. – 2018. – Vol. 102. – no. 14. – P. 5873–5888.

Costs of Inaction on the Sound Management of Chemicals // Electronic resource UNEP Official website. – 2013. URL: http://www.unep.org/chemicalsandwaste/

de Faria M. R., Wraight S. P. Mycoinsecticides and mycoacaricides: a comprehensive list with worldwide coverage and international classification of formulation types //Biological control. – 2007. – Vol. 43. – no. 3. – P. 237–256.

Fan Y. Regulatory cascade and biological activity of Beauveria bassiana oosporein that limits bacterial growth after host death //Proceedings of the National Academy of Sciences. – 2017. – Vol. 114. – no. 9. – P. E1578–E1586.

Glare T. Have biopesticides come of age? //Trends in biotechnology. – 2012. – Vol. 30. – no. 5. – P. 250–258.

Goettel, M.S., Eilenberg, J., Glare, T.R. Entomopathogenic fungi and their role in regulation of insect populations. In: Gilbert, L.I., Gill, S.S. (Eds.), Insect Control: Biological and Synthetic Agents //Academic Press, London. – 2010. – P. 387–432.

Gouli V., Gouli S., Kim J. S. Production of Beauveria bassiana air conidia by means of optimization of biphasic system technology //Brazilian Archives of Biology and Technology. – 2014. – Vol. 57. – P. 571–577.

Hill D. S. Pests of crops in warmer climates and their control. – Springer Science & Business Media, 2008.

Javed K. efficacy of Beauveria bassiana and Verticillium lecanii for the management of whitefly and aphid //Pakistan Journal of Agricultural Sciences. – 2019. – Vol. 56. – no. 3. P. 274–287.

Jon A. Martin & Laurence A. Mound. An annotated check list of the world's whiteflies (Insecta: Hemiptera: Aleyrodidae)

// Zootaxa : journal. – 2007. – Vol. 1492. – P. 1–84. – ISBN 978-1-86977-112-6.

Kapongo J. P. Optimal concentration of Beauveria bassiana vectored by bumble bees in relation to pest and bee mortality in greenhouse tomato and sweet pepper //BioControl. – 2008. – Vol. 53. – no. 5. – P. 797–812.

Keyhani N. O. Lipid biology in fungal stress and virulence: Entomopathogenic fungi //Fungal biology. – 2018. – Vol. 122.

– no. 6. – P. 420–429.

Ortiz-Urquiza A., Keyhani N. O. Action on the surface: entomopathogenic fungi versus the insect cuticle //Insects. – 2013.

– Vol. 4. – no. 3. – P. 357–374.

Ortiz-Urquiza A., Luo Z., Keyhani N. O. Improving mycoinsecticides for insect biological control //Applied microbiology and biotechnology. – 2015. – Vol. 99. – no. 3. – P. 1057–1068.

Pedrini N. Targeting of insect epicuticular lipids by the entomopathogenic fungus Beauveria bassiana: hydrocarbon oxidation within the context of a host-pathogen interaction //Frontiers in microbiology. – 2013. – Vol. 4. – P. 24.

Pedrini N., Crespo R., Juárez M. P. Biochemistry of insect epicuticle degradation by entomopathogenic fungi //Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. – 2007. – Vol. 146. – no. 1-2. – P. 124–137.

Quesada-Moraga E. Selection of Beauveria bassiana isolates for control of the whiteflies Bemisia tabaci and Trialeurodes vaporariorum on the basis of their virulence, thermal requirements, and toxicogenic activity //Biological control. – 2006. – Vol. 36.

– no. 3. – P. 274–287.

Shcherbakov D. The most primitive whiteflies (Hemiptera; Aleyrodidae; Bernaeinae subfam. nov.) from the Mesozoic of Asia and Burmese amber, with an overview of Burmese amber hemipterans // Bulletin of the British Museum (Natural History). Geology. – 2000. – Vol. 56, no. 1. – P. 29–37.

St Leger R. J., Wang C. Genetic engineering of fungal biocontrol agents to achieve greater efficacy against insect pests // Applied Microbiology and Biotechnology. – 2010. – Vol. 85. – no. 4. – P. 901–907.

St Leger R. J., Wang C. Genetic engineering of fungal biocontrol agents to achieve greater efficacy against insect pests // Applied Microbiology and Biotechnology. – 2010. – Vol. 85. – no. 4. – P. 901–907.

Tarocco F. Optimization of erythritol and glycerol accumulation in conidia of Beauveria bassiana by solid-state fermentation, using response surface methodology //Applied microbiology and biotechnology. – 2005. – Vol. 68. – no. 4. – P. 481–488.

Valero-Jiménez C. A. Genes involved in virulence of the entomopathogenic fungus Beauveria bassiana //Journal of Invertebrate Pathology. – 2016. – Vol. 133. – P. 41–49.

Wraight, S.P., G.D. Inglis and M.S. Goettel. Fungi. In: Lacey, L.A., Kaya, H.K. (Eds.), Field Manual of Techniques in Invertebrate Pathology: Application and Evaluation of Pathogens for Control of Insects and Other Invertebrate Pests// Springer, Dordrecht, the Netherlands. – 2007, – P.223–248.

Wright M. S., Lax A. R. Improved mortality of the Formosan subterranean termite by fungi, when amended with cuticle- degrading enzymes or eicosanoid biosynthesis inhibitors //Folia microbiologica. – 2016. – Vol. 61. – no. 1. – P. 73–83.

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Published

2022-04-01

How to Cite

Doolotkeldieva Т., Zholdoshbekova, C., & Ismailova Э. (2022). BIOTESTING OF ENTOMOPATHOGENIC FUNGI BEAUVERIA BASSIANA AGAINST THE WHITEFLY (TRIALEURODES VAPORARIORUM): LABORATORY AND FIELD EXPERIMENTS. Experimental Biology, 90(1), 50–59. https://doi.org/10.26577/eb.2022.v90.i1.05