Elaboration of technology for obtaining of genetically modified canola plants

Authors

  • R. M. Nargilova Институт молекулярной биологии и биохимии им. М.А. Айтхожина, г. Алматы, Казахстан
  • O. V. Karpova Институт молекулярной биологии и биохимии им. М.А. Айтхожина, г. Алматы, Казахстан
  • A. M. Pissarenko Институт молекулярной биологии и биохимии им. М.А. Айтхожина, г. Алматы, Казахстан
  • B. K. Iskakov Институт молекулярной биологии и биохимии им. М.А. Айтхожина, г. Алматы, Казахстан

Keywords:

Brassica napus, canola, regeneration, transformation, AtDREB1A

Abstract

The task of this work is optimization of the conditions for regeneration of different tissue and obtaining of transgenic rape plants (Brassica napus L.) after transformation by Agrobacterium tumerfaciens using of transcription factor AtDREB1A gene.The method of rape regeneration has developed using hypocotyls and cotyledons obtained from germinated seeds. By virtue of this development the conditions were selected for stable genetic transformation of cotyledons and hypocotyls by Agrobacterium strains. As results transgenic rape plants with the recombinant DNA sequences have obtained.

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Section

Biotechnology: from research to innovations

How to Cite

Elaboration of technology for obtaining of genetically modified canola plants. (2015). Experimental Biology, 59(3/1), 149-152. https://bb.kaznu.kz/index.php/biology/article/view/673

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