Investigation of ability of the grapevine virus A to infect Nicotiana Benthamiana by using Agrobacterium infiltration

Abstract

In this work was created binary vector carrying the complete genome of an infectious clone of the virus A grape with flanking regulatory sequences (35S- promoter , 35S- terminator ). Agrobacterium was transformed by this vector for delivery viral genome into plants by using agroinfiltration . Agroinfiltration was carried by bacteria of different optical density, in order to identify the optimum density for tobacco. Early signs of infection were noticed at 7-10 days after agroinfiltration in plants, which confirms the continuation of infectivity of the virus.