Optimization of conditions for cryopreservation of isolated pear meristems in liquid nitrogen.
Ключевые слова:
Cryopreservation, pear, meristem, cryoprotectant, acclimation,Аннотация
The effect of the duration of hardening of test-tube plants and thawing methods on growth recovery of cryopreserved pear meristems was defined. Optimum hardening is 3-4 weeks with varying temperature and irradiance (8 h, at 22°C, with 7 μmol m-2s-1 irradiance, followed 16 h at -1°C without irradiance). The effect of different cryoprotectants on the viability of meristems after freezing in liquid nitrogen was studied. The best cryoprotectant for cryopreservation of isolated pear meristems is IBBR-1 (50% glycerol and 50% glucose). Recovering nutrient medium composition for regeneration in vitro cryopreserved pear meristems was developed.Библиографические ссылки
1 Reed B.M. Cryopreservation of Temperate Berry Crops // Plant Cryopreservation: A Practical Guide. – New York:
Springer Science + Business Media LLC, 2008. – P. 333-364.
2 Chang Y., Reed B.M.. Extended cold acclimation and recovery medium alteration improve regrowth of Rubus shoot tips following cryopreservation // Cryo-Letters. – 1999. - № 20. – P. 371-376.
3 Hirai D., Sakai A. Cryopreservation of in vitro grown meristems of potato by encapsulation-vitrification // Cryopreservation of tropical plant germplasm. – 2000. –V. 89. - P. 205-211.
4 Пат. 68746 Казахстан. Состав криопротектора для замораживания меристем черной смородины в жидком
азоте / Турдиев Т.Т., Ковальчук И.Ю.; № гос. регистрации заявки 2010/0734.1 от 02.06.2010.
Springer Science + Business Media LLC, 2008. – P. 333-364.
2 Chang Y., Reed B.M.. Extended cold acclimation and recovery medium alteration improve regrowth of Rubus shoot tips following cryopreservation // Cryo-Letters. – 1999. - № 20. – P. 371-376.
3 Hirai D., Sakai A. Cryopreservation of in vitro grown meristems of potato by encapsulation-vitrification // Cryopreservation of tropical plant germplasm. – 2000. –V. 89. - P. 205-211.
4 Пат. 68746 Казахстан. Состав криопротектора для замораживания меристем черной смородины в жидком
азоте / Турдиев Т.Т., Ковальчук И.Ю.; № гос. регистрации заявки 2010/0734.1 от 02.06.2010.
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