Effect of bioactive substances from Limonium gmelinii (Plumbaginaceae) and Inula britannica L. (Compositae) on anti-oxidative status of barley seedlings at asymmetric dymethylhydrazine effects

Abstract

Plant resistance to environmental stress factors largely depends on the state of the antioxidant system (AOS). Essential antioxidants of plants as a specialized enzyme systems (superoxide dismutase, catalase, etc.) directly effect on reactive oxygen species are liable to hinder or eliminate free radical oxidation of organic substances. AOS enzymes are involved in the regulation of metabolism and are of particular importance in enabling rapid adaptation to constantly changing external conditions of environment. The aim of our research was to study the influence of different concentrations of bioactive substances extracted from aboveground and underground parts of Limonium gmelinii and Inula britannica on the functioning of antioxidative enzymes of barley seedlings under the unsymmetrical dymethylhydrazine (UDMH). It is found that UDMH activated protective antioxidant system in 2-day barley sprouts. Revealed a high content of malondialdehyde and increasing catalase activity compared with control. The combined impact of UDMH and bioactive substances of plants reduce the intensity of lipid peroxidation and catalase activity in barley sprouts. This directly indicates that bioactive substances of Limonium gmelinii and Inula britannica have adaptogenic properties.