Optimization of conditions for cryopreservation of isolated pear meristems in liquid nitrogen.

I. Y. Kovalchuk; Zh. B. Zhumagulova; G. A. Madiyeva; Т. Т. Turdiev; S. N. Frolov

Authors

I. Y. Kovalchuk Институт биологии и биотехнологии растений, г. Алматы
Zh. B. Zhumagulova Институт биологии и биотехнологии растений, г. Алматы
G. A. Madiyeva Институт биологии и биотехнологии растений, г. Алматы
Т. Т. Turdiev Институт биологии и биотехнологии растений, г. Алматы
S. N. Frolov Институт биологии и биотехнологии растений, г. Алматы

Keywords:

Cryopreservation, pear, meristem, cryoprotectant, acclimation,

Abstract

The effect of the duration of hardening of test-tube plants and thawing methods on growth recovery of cryopreserved pear meristems was defined. Optimum hardening is 3-4 weeks with varying temperature and irradiance (8 h, at 22°C, with 7 μmol m-2s-1 irradiance, followed 16 h at -1°C without irradiance). The effect of different cryoprotectants on the viability of meristems after freezing in liquid nitrogen was studied. The best cryoprotectant for cryopreservation of isolated pear meristems is IBBR-1 (50% glycerol and 50% glucose). Recovering nutrient medium composition for regeneration in vitro cryopreserved pear meristems was developed.