МИКРОКЛОНАЛЬНОЕ РАЗМНОЖЕНИЕ ВИНОГРАДА НА РАЗЛИЧНЫХ ПИТАТЕЛЬНЫХ СРЕДАХ

Abstract

The present work represents the results of micropropagation of European perspective grapevine cultivars in two media: IM and MS, both supplemented with 4,4 μmol BAP and 0,05 μmol NAA, but with different amounts of KNO3 and NH4NO3. Dormant buds of Saperavi, Cabernet franc and Gewürztraminer cultivars were used as explants. In experiments the survival for all used cultivars after transfer from IM medium to ex vitro was as more as several times compared to those from MS medium. Saperavi in IM media showed maximum of microshoots formation possibility and adaptation possibility in substrate (soil/peat/zeolite (1/1/1)) compared to Cabernet franc and Gewürztraminer. The survival of plantlets, after transfer from IM to substrate, increased more than 30% for Saperavi and Cabernet, and 25% for Gewürztraminer. After 3-4 passages, multiplied microshoots were separated and transferred in rooting medium ½ MS with 5,7 μM IAA, 20 g sucrose, 0.6 % agar, 2 ml/l glycine, 50 μl thiamine hydrochloride, 250 μl pyridoxine and 250 μl nicotinic acid. Depending on cultivars in IM medium after apex’s excision and following formation of axillary microshoots from 3 to 10 rooted plantlets have been obtained. More than 600 rooted plantlets after acclimatization ex situ were transferred to the experimental field for surveillance of overwinter survival.