Молекулярно-генетический анализ новых изолятов вируса гриппа птиц субтипа Н3N8, выделенных в северных регионах Казахстана

K. T. Sultankulova; K. K. Akylbayeva; K. K. Jekebekov; T. A. Junushov; A. M. Melisbek; I. A Zuban’; M. B. Orynbayev; K. D. Zakarya

doi:10.26577//eb.2020.v82.i1.10

Authors

K. T. Sultankulova Research Institute of Biological Safety Problems, Kazakhstan, Zhambyl oblast, Gvardeiskiy vil.
K. K. Akylbayeva Research Institute of Biological Safety Problems, Kazakhstan, Zhambyl oblast, Gvardeiskiy vil.
K. K. Jekebekov Research Institute of Biological Safety Problems, Kazakhstan, Zhambyl oblast, Gvardeiskiy vil.
T. A. Junushov Institute of biotechnology Of the national Academy of Sciences of the Kyrgyz Republic
A. M. Melisbek Research Institute of Biological Safety Problems, Kazakhstan, Zhambyl oblast, Gvardeiskiy vil.
I. A Zuban’ North Kazakhstan State University named after M. Kozybaeva
M. B. Orynbayev Research Institute of Biological Safety Problems, Kazakhstan, Zhambyl oblast, Gvardeiskiy vil.
K. D. Zakarya Research Institute of Biological Safety Problems, Kazakhstan, Zhambyl oblast, Gvardeiskiy vil.

DOI:

https://doi.org/10.26577//eb.2020.v82.i1.10

Abstract

Molecular and genetic analysis of viral isolates genes will allow to estimate the spread of influenza A virus in the territory of the Republic of Kazakhstan. Avian influenza control is difficult due to the high variability of the virus genome. The influenza virus circulation among wild waterfowl and the proximity of humans to their habitats is a potential source of threat to public health. Currently, the influenza agent is the subject of detailed study, especially in terms of genetic certification of strains used for the development of diagnostic and prophylactic agents.

The aim is studying the molecular genetic properties of new isolates of avian influenza virus subtype H3N8 isolated during the monitoring of avian influenza in the Northern regions of Kazakhstan. Modern methods of molecular biology and genetic engineering were used in the research.

In 2018, virological monitoring of wild birds in the Northern regions of Kazakhstan revealed individuals infected with influenza a virus. Pharyngeal swabs of two birds in particular shovelers (Anas clypeata) and teal cracker (Anas querquedula) showed positive reactions for influenza virus type A, subtype H3N8 by PCR.

The study obtained isolates of influenza virus A/shoveler/SKO/20/2018 (H3N8) and A/Teal cracker/RMS/45/2018 (H3N8).

Results of determining nucleotide sequences and phylogenetic analysis of the new isolates important genes, A/shoveler/SKO/20/2018 (H3N8) and A/Teal cracker/RMS/45/2018 (H3N8) influenza virus in birds are shown. New Kazakhstan isolates of avian influenza virus on the composition of M-gene show the greatest affinity with the strains of avian influenza virus subtype H3N8 isolated in Mongolia in 2018.

The molecular genetic analysis results of new isolates of the H3N8 subtype avian influenza virus isolated in the Northern regions of Kazakhstan will be used in the new generation of vaccines production, diagnostics and development of measures to improve the influenza epizootic situation in Kazakhstan.

Key words: genetic analysis, avian influenza virus, wild birds, PCR, RNA