Антибактериальная активность эфирных масел некоторых видов Artemisia и Thymus в отношении метициллин-резистентного Staphylococcus aureus

G. A. Utegenova; K. B. Pallister; J. M. Voyich; G. Ozek; T. Ozek; L. N. Kirpotina; I. A. Schepetkin; S. V. Kushnarenko

Authors

G. A. Utegenova Al-Farabi Kazakh National University, Kazakhstan, Almaty
K. B. Pallister Montana State University, The United States of America, Bozeman
J. M. Voyich Montana State University, The United States of America, Bozeman
G. Ozek Anadolu University, Republic of Turkey, Eskishehir
T. Ozek Anadolu University, Republic of Turkey, Eskishehir
L. N. Kirpotina Montana State University, The United States of America, Bozeman
I. A. Schepetkin Tomsk Polytechnic University, The Russian Federation, Tomsk
S. V. Kushnarenko Institute of Plant Biology and Biotechnology, Republic of Kazakhstan, Almaty

Keywords:

essential oils, antibacterial activity, methicillin-resistant Staphylococcus aureus (MRSA).

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is the causative agent of many diseases, firstly, of skin and soft tissues infections. The emergence of antibiotic-resistant strains, such as MRSA requires the search for new agents to withstand this pathogen, in particular among natural compounds. Essential oils are natural organic compounds that have a wide spectrum of biological activity: antimicrobial, antiviral, antioxidant, anti-inflammatory and immunomodulattory. The present work for the first time has determined an antibacterial activity of essential oils isolated from 5 plants of Kazakhstan: Artemisia kotuchovii Kupr., Artemisia scoparia Waldst. et Kit., Thymus crebrifolius (Klokov), Thymus marschallianus Willd. and Thymus rasitatus (Klokov) in regard to MRSA USA300. Antibacterial activity of essential oils was defined by measuring the light absorption kinetics of bacterial suspension using calculation of the inhibitory concentration (IC₂₅) at which the growth of microorganisms was inhibited to 25%. A. scoparia, T. marschallianus and Т. rasitatus essential oils (IС₂₅= 24.5; 30.9 and 32.7 μg/ml, respectively) possess the high inhibitory effect on increase of MRSA; T. crebrifolius essential oil showed low activity (IС₂₅= 73.0 μg/ml). A. kotuchovii essential oil did not show activity even at the highest tested concentration of 100 μg/ml.