Isolation and identification of new cultures of toxin-forming cyanobacteria from the Shar Nuur Lake
Keywords:
cyanobacteria, bacteriologically pure culture, morphology, toxins, identification, Desertifilum sp.1 strain.Abstract
Studied the species composition of algal flora of Shar Nuur Lake, located in the mountainous regions of Bayan Ulgiisk region of Mongolia. From selected water samples and algal-bacterial mats received 3 bacteriologically pure cultures of cyanobacteria from family Oscillatoriaceae and Nostocaceae. Determined the toxicity of cyanobacteria’ selected strains according to the system of N.S. Stroganov. It was found that from cyanobacteria’ isolated strains the culture SP-O1 is defined as highly-toxic to the test-object - Daphnia. Evaluation of biological activity of toxic cyanobacteria cultures studied in relation to the test-object cell lines HeLa cancer cells showed different cytotoxic effect. In studied extracts of Desertifilum SP-O1 biomass strain the dangerous toxins were not detected. Mostly identified toxins are microcystins. Despite on the fact that according to botanical characteristics the obtained cyanobacteria SP-O1 from Shar Nuur Lake is related to the Oscillatoria genus, molecular-genetic analysis revealed its high homology to the Desertifilum genus belonging to Oscillatoriaceae family. Based on these data, SP-O1 culture was identified and designated as Desertifilum sp.1.
