Комбинированное воздействие рентгеновского и γ- излучений на клетки периферической крови человека in vivo и in vitro
58 35
Ключевые слова:
Хромосомные аберрации, радиочувствительность, адаптивный ответ, γ-излучение, рентгеновское излучение.Аннотация
Представлены результаты изучения in vivo эффектов хронического влияния рентгеновского излучения на медицинских работников рентгенлабораторий, а также оценена радиочувствительность и способность лимфоцитов их периферической крови к формированию адаптивного ответа под действием дополнительного γ-излучения. Проведены in vitro эксперименты по изучению комбинированного воздействия Х- и γ- излучений на клетки периферической крови здоровых доноров. Изучение спонтанного уровня частоты хромосомных нарушений выявило значительное повышение аберраций как хромосомного так и хроматидного типов у обследованной группы рентгенологов по сравнению с контролем, что свидетельствует о мутагенном эффекте факторов профессиональной среды. Корреляции между возрастом, продолжительностью работы во вредных условиях и выходом хромосомных нарушений не выявлено. Обнаружена высокая радиочувствительность к дополнительному γ-излучению и индукция большого количества мультиаберрантных клеток, которые у отдельных индивидуумов составляли до 30% от количества клеток, содержащих хромосомные нарушения. Тем не менее, у некоторых лиц сохраняется способность клеток к формированию реакции адаптивного ответа, что выражается в снижении общей частоты хромосомных аберраций, но не аберрантных клеток. В in vitro экспериментах с дробным облучением в адаптирующих дозах крови здоровых доноров рентгеновскими лучами и облучение этой же крови повреждающей дозой γ-излучения показывает, что адаптивный ответ формируется только при однократном облучении адаптирующей дозой. Фракционированное Х-излучение способствует развитию радиосенсибилизации, т. е. оно подавляет защитные функции клеток, в частности адаптивный ответ, и наблюдается тенденция к увеличению частоты аберраций на одну аберрантную клетку.
Библиографические ссылки
1. Zasukhina GD (2008) The adaptive response - general biological rule: facts, hypotheses, questions. Radiation Biology. Radioecology [Adaptivnyi otvet – obshchebiologicheskaia zakonomernost': fakty, gipotezy, voprosy. Radiatsionnaia biologiia. Radioekologiia] 4:464-473. (In Russian)
2. Monastyrskaia EA, Andreeva LV, Duchen MR (2003) Direct and cross-protective effects of adaptation to heat in the cell culture. Bulletin of Experimental Biology and Medicine [Priamye i perekrestnye zashchitnye effekty adaptatsii k teplu v kul'ture kletok. Biullleten' eksperimental'noi biologii i meditsiny] 2:147-150. (In Russian)
3. Bondarchuk IA (2003) Analysis of the role of DNA repair, cell cycle regulation and apoptosis in a radiation-induced adaptive response of mammalian cells. Radiation Biology. Radioecology [Analiz roli reparatsii DNK, reguliatsii kletochnogo tsikla i apoptoza v radiatsionno-indutsirovannom adaptivnom otvete kletok mlekopitaiushchikh. Radiatsionnaia biologiia. Radioekologiia] 1:19-28. (In Russian)
4. Akhmatullina NB, Makarenko NG, Iskandarova KA, Cherednichenko OG, Kim S (2002) Mathematical analysis of the effects of low doses of radiation. Radiation Biology. Radioecology [Matematicheskii analiz effektov malykh doz radiatsii. Radiatsionnaia biologiia. Radioekologiia] 6:614-617. (In Russian)
5. Moorhead PS, Nowell PC, Mellman WJ (1960) Chromosome preparations of leucocytes cultured from human peripheral blood, Experimental Cell Research, 20: 613-616. DOI:10.1016/0014-4827(60)90138-5
6. Plokhinskii NA (1967) Algorithms biometrics [Alroritmy biometrii]. Moscow, Russia. ISBN 1702060000. (In Russian)
7. Fenech M, Chang W P, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E (2003) HUMN project: detailed description of thescoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures, Mutat. Res., 534:65—75. DOI:10.1016/S1383-5718(02)00249-8
8. Iablokov A.V. (2002) The myth of small doses of radiation safety [Mif o bezopasnosti malykh doz radiatsii]. Moscow, Russia. ISBN 5 - 901815 - 04. (In Russian)
9. Rubanovich AV(2006) The theory and practice of construction of calibration curves in Biodosimetry. Radiation Biology. Radioecology [Teoriia i praktika postroeniia kalibrovochnykh krivykh v biodozimetrii. Radiatsionnaia biologiia. Radioekologiia] 46:447-456. (In Russian)
10. Gaziev AI (2011) Low efficiency of DNA repair critical damage caused by small radiation doses. Radiation Biology. Radioecology [Nizkaia effektivnost' reparatsii kriticheskikh povrezhdenii DNK, vyzyvaemykh malymi dozami radiatsii. Radiatsionnaia biologiia. Radioekologiia] 5:512-529. (In Russian)
11. Darenskaia NG, Korotkevich AO, Maliutina TS, Nasonova TA (2000) Individual radioresistance and the principles of its formation. Aerospace and Environmental Medicine [Individual'naia radiorezistentnost' i printsipy ee formirovaniia. Aviakosmicheskaia i ekologicheskaia meditsina] 5:32-37. (In Russian)
12. Serebrianyi AM, Aleshchenko AV, Gotlib VIa, Kudriashova OV, Semenova LP, Pelevina II (2011) Radiation adaptive response as stress-response cells. Radiation Biology. Radioecology [Radiatsionnyi adaptivnyi otvet kak stress-reaktsiia kletki. Radiatsionnaia biologiia. Radioekologiia] 4:399-404. (In Russian)
13. Pelevina I I, Aleshchenko AA, Antoshchina MM, Riabchenko NI, Semenova LP, Serebrianyi AM (2007) Individual variability in the manifestation of the adaptive response of human cells to the effects of ionizing radiation. The approaches to its definition. Radiation Biology. Radioecology [Individual'naia variabel'nost' v proiavlenii adaptivnogo otveta kletok cheloveka na vozdeistvie ioniziruiushchei radiatsii. Podkhody k ee opredeleniiu. Radiatsionnaia biologiia. Radioekologiia] 6: 658-666. (In Russian)
14. Akleev AV, Aleshchenko AV, Gotlib VIa, Kudriashova OV, Semenova LP, Serebrianyi AM, Khudiakova OI, Pelevina II (2004) Adaptive capacity of lymphocytes in the blood of inhabitants of the Southern Urals, exposed to chronic radiation. Radiation Biology. Radioecology [Adaptivnye sposobnosti limfotsitov krovi u zhitelei Iuzhnogo Urala, podvergshikhsia khronicheskomu oblucheniiu. Radiatsionnaia biologiia. Radioekologiia] 4:426-431. (In Russian)
15. Vasil'eva IM, Unzhakov SV, Meliksetova IA (1995) The adaptive response in lymphocytes of children from areas with high background ionizing radiation. Radiation Biology. Radioecology [Adaptivnyi otvet v limfotsitakh detei iz zony s povyshennym fonom ioniziruiushchei radiatsii. Radiatsionnaia biologiia. Radioekologiia] 5:662-664. (In Russian)
16. Aseeva EA, Snigireva GP, Neverova AL, Novitskaia NN, Khazins ED, Domracheva EV (2009) Cells with multiple chromosomal abnormalities in a group of persons exposed to radiation in different situations, and their possible biological role. Radiation Biology. Radioecology [Kletki s mnozhestvennymi khromosomnymi narusheniiami v gruppakh lits, podvergshikhsia oblucheniiu pri razlichnykh situatsiiakh, i ikh vozmozhnaia biologicheskaia rol'. Radiatsionnaia biologiia. Radioekologiia] 5:552-562. (In Russian)
17. Domracheva EV, Rivkind NB, Shklovskii-Kordi NE, Vorob'ev AI (1997) Multi Aberrant cells from residents of the territories contaminated with radionuclides due to the accident at the Chernobyl nuclear power plant, and the liquidators of the accident. Problems of Hematology and Blood Transfusion [Mul'tiaberrantnye kletki u zhitelei territorii, zagriaznennykh radionuklidami v rezul'tate avarii na Chernobyl'skoi atomnoi stantsii, i uchastnikov likvidatsii etoi avarii. Problemy gematologii i perelivaniia krovi] 2:12-19. (In Russian)
18. Petin VG, Zhuravskaia GL (2015) Influence of intensity of action of the agent on the manifestation of synergy. Radiation Biology. Radioecology [Vliianie intensivnosti deistvuiushchikh agentov na proiavlenie sinergicheskogo vzaimodeistviia . Radiatsionnaia biologiia. Radioekologiia] 6:598-607. (In Russian)
19. Pelevina II, Aleshchenko AV, Antoshchina MM, Biriukov VA, Reva EV, Minaeva NG (2015) Change radiosensitivity after irradiation in small doses, possible mechanisms and patterns. Radiation Biology. Radioecology [Izmenenie radiochuvstvitel'nosti posle oblucheniia v malykh dozakh, vozmozhnye mekhanizmy i zakonomernosti. Radiatsionnaia biologiia. Radioekologiia] 1:57-62. (In Russian)
20. Pelevina II, Oradovskaia IV, Mansurova IuG, Aleshchenko AV, Antoshchina MM, Kudriashova OV, Lizunova E Iu, Nikonova MF, Osipov AN, Riabchenko N I, Feoktistov VV, Serebrianyi A M (2010) Communication molecular cell performance and immune status of blood lymphocytes accident liquidators of the Chernobyl. Radiation Biology. Radioecology [Sviaz' molekuliarno-kletochnykh pokazatelei i immunnogo statusa u limfotsitov krovi likvidatorov avarii na ChAES. Radiatsionnaia biologiia. Radioekologiia] 5:501-507. (In Russian)
21. Serebrianyi AM, Antoshchina MM, Aleshchenko AV, Kudriashova OV, Nikonova MF, Riabchenko NI, Iarilin AA (2013) Cytogenetic and immunological parameters stimulated human peripheral blood lymphocytes are associated with cell proliferation rate. Radiation Biology. Radioecology [Tsitogeneticheskie i immunologicheskie pokazateli stimulirovannykh limfotsitov perifericheskoi krovi cheloveka sviazany so skorost'iu proliferatsii kletok. Radiatsionnaia biologiia. Radioekologiia] 6:549-555. (In Russian)
22. Osipov AN, Lizunova EIu, Vorob'eva NIu, Pelevina II (2009) Induction and repair of double-strand DNA breaks in human lymphocytes irradiated in a dose of adapting. Radiation Biology. Radioecology [Induktsiia i reparatsiia dvunitevykh razryvov DNK v limfotsitakh krovi cheloveka, obluchennykh v adaptiruiushchei doze. Radiatsionnaia biologiia. Radioekologiia] 1:42-45. (In Russian)
23. Mikhailov BF, Mazurik VK, Ushenkova LN, Nadezhina NM, Galstian .A, Raeva NF (2006) The study of molecular manifestations of genomic instability in persons exposed to ionizing radiation at clinically relevant doses. Radiation Biology. Radioecology [Issledovanie molekuliarnykh proiavlenii nestabil'nosti genoma u lits, podvergavshikhsia vozdeistviiu ioniziruiushchei radiatsii v klinicheski znachimykh dozakh. Radiatsionnaia biologiia. Radioekologiia] 3:322-336. (In Russian)
24. Akleev AV, Aleshchenko AV, Kudriashova OV, Semenova LP, Serebrianyi AM, Khudiakova OI, Pelevina II (2011) The adaptive response of blood lymphocytes as an indicator of the state of hematopoiesis in irradiated individuals. Radiation Biology. Radioecology [Адаптивный ответ лимфоцитов крови как индикатор состояния гемопоэза у облученных лиц. Radiatsionnaia biologiia. Radioekologiia] 6:645-650. (In Russian)
25. Khandogina EK, Mutovin GR, Zvereva SV, Antipov AV (1991) Adaptive response in irradiated human lymphocytes: radiobiological and genetic aspects, Mutat.Res., 251:181-186. DOI:10.1016/0027-5107(91)90073-W
2. Monastyrskaia EA, Andreeva LV, Duchen MR (2003) Direct and cross-protective effects of adaptation to heat in the cell culture. Bulletin of Experimental Biology and Medicine [Priamye i perekrestnye zashchitnye effekty adaptatsii k teplu v kul'ture kletok. Biullleten' eksperimental'noi biologii i meditsiny] 2:147-150. (In Russian)
3. Bondarchuk IA (2003) Analysis of the role of DNA repair, cell cycle regulation and apoptosis in a radiation-induced adaptive response of mammalian cells. Radiation Biology. Radioecology [Analiz roli reparatsii DNK, reguliatsii kletochnogo tsikla i apoptoza v radiatsionno-indutsirovannom adaptivnom otvete kletok mlekopitaiushchikh. Radiatsionnaia biologiia. Radioekologiia] 1:19-28. (In Russian)
4. Akhmatullina NB, Makarenko NG, Iskandarova KA, Cherednichenko OG, Kim S (2002) Mathematical analysis of the effects of low doses of radiation. Radiation Biology. Radioecology [Matematicheskii analiz effektov malykh doz radiatsii. Radiatsionnaia biologiia. Radioekologiia] 6:614-617. (In Russian)
5. Moorhead PS, Nowell PC, Mellman WJ (1960) Chromosome preparations of leucocytes cultured from human peripheral blood, Experimental Cell Research, 20: 613-616. DOI:10.1016/0014-4827(60)90138-5
6. Plokhinskii NA (1967) Algorithms biometrics [Alroritmy biometrii]. Moscow, Russia. ISBN 1702060000. (In Russian)
7. Fenech M, Chang W P, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E (2003) HUMN project: detailed description of thescoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures, Mutat. Res., 534:65—75. DOI:10.1016/S1383-5718(02)00249-8
8. Iablokov A.V. (2002) The myth of small doses of radiation safety [Mif o bezopasnosti malykh doz radiatsii]. Moscow, Russia. ISBN 5 - 901815 - 04. (In Russian)
9. Rubanovich AV(2006) The theory and practice of construction of calibration curves in Biodosimetry. Radiation Biology. Radioecology [Teoriia i praktika postroeniia kalibrovochnykh krivykh v biodozimetrii. Radiatsionnaia biologiia. Radioekologiia] 46:447-456. (In Russian)
10. Gaziev AI (2011) Low efficiency of DNA repair critical damage caused by small radiation doses. Radiation Biology. Radioecology [Nizkaia effektivnost' reparatsii kriticheskikh povrezhdenii DNK, vyzyvaemykh malymi dozami radiatsii. Radiatsionnaia biologiia. Radioekologiia] 5:512-529. (In Russian)
11. Darenskaia NG, Korotkevich AO, Maliutina TS, Nasonova TA (2000) Individual radioresistance and the principles of its formation. Aerospace and Environmental Medicine [Individual'naia radiorezistentnost' i printsipy ee formirovaniia. Aviakosmicheskaia i ekologicheskaia meditsina] 5:32-37. (In Russian)
12. Serebrianyi AM, Aleshchenko AV, Gotlib VIa, Kudriashova OV, Semenova LP, Pelevina II (2011) Radiation adaptive response as stress-response cells. Radiation Biology. Radioecology [Radiatsionnyi adaptivnyi otvet kak stress-reaktsiia kletki. Radiatsionnaia biologiia. Radioekologiia] 4:399-404. (In Russian)
13. Pelevina I I, Aleshchenko AA, Antoshchina MM, Riabchenko NI, Semenova LP, Serebrianyi AM (2007) Individual variability in the manifestation of the adaptive response of human cells to the effects of ionizing radiation. The approaches to its definition. Radiation Biology. Radioecology [Individual'naia variabel'nost' v proiavlenii adaptivnogo otveta kletok cheloveka na vozdeistvie ioniziruiushchei radiatsii. Podkhody k ee opredeleniiu. Radiatsionnaia biologiia. Radioekologiia] 6: 658-666. (In Russian)
14. Akleev AV, Aleshchenko AV, Gotlib VIa, Kudriashova OV, Semenova LP, Serebrianyi AM, Khudiakova OI, Pelevina II (2004) Adaptive capacity of lymphocytes in the blood of inhabitants of the Southern Urals, exposed to chronic radiation. Radiation Biology. Radioecology [Adaptivnye sposobnosti limfotsitov krovi u zhitelei Iuzhnogo Urala, podvergshikhsia khronicheskomu oblucheniiu. Radiatsionnaia biologiia. Radioekologiia] 4:426-431. (In Russian)
15. Vasil'eva IM, Unzhakov SV, Meliksetova IA (1995) The adaptive response in lymphocytes of children from areas with high background ionizing radiation. Radiation Biology. Radioecology [Adaptivnyi otvet v limfotsitakh detei iz zony s povyshennym fonom ioniziruiushchei radiatsii. Radiatsionnaia biologiia. Radioekologiia] 5:662-664. (In Russian)
16. Aseeva EA, Snigireva GP, Neverova AL, Novitskaia NN, Khazins ED, Domracheva EV (2009) Cells with multiple chromosomal abnormalities in a group of persons exposed to radiation in different situations, and their possible biological role. Radiation Biology. Radioecology [Kletki s mnozhestvennymi khromosomnymi narusheniiami v gruppakh lits, podvergshikhsia oblucheniiu pri razlichnykh situatsiiakh, i ikh vozmozhnaia biologicheskaia rol'. Radiatsionnaia biologiia. Radioekologiia] 5:552-562. (In Russian)
17. Domracheva EV, Rivkind NB, Shklovskii-Kordi NE, Vorob'ev AI (1997) Multi Aberrant cells from residents of the territories contaminated with radionuclides due to the accident at the Chernobyl nuclear power plant, and the liquidators of the accident. Problems of Hematology and Blood Transfusion [Mul'tiaberrantnye kletki u zhitelei territorii, zagriaznennykh radionuklidami v rezul'tate avarii na Chernobyl'skoi atomnoi stantsii, i uchastnikov likvidatsii etoi avarii. Problemy gematologii i perelivaniia krovi] 2:12-19. (In Russian)
18. Petin VG, Zhuravskaia GL (2015) Influence of intensity of action of the agent on the manifestation of synergy. Radiation Biology. Radioecology [Vliianie intensivnosti deistvuiushchikh agentov na proiavlenie sinergicheskogo vzaimodeistviia . Radiatsionnaia biologiia. Radioekologiia] 6:598-607. (In Russian)
19. Pelevina II, Aleshchenko AV, Antoshchina MM, Biriukov VA, Reva EV, Minaeva NG (2015) Change radiosensitivity after irradiation in small doses, possible mechanisms and patterns. Radiation Biology. Radioecology [Izmenenie radiochuvstvitel'nosti posle oblucheniia v malykh dozakh, vozmozhnye mekhanizmy i zakonomernosti. Radiatsionnaia biologiia. Radioekologiia] 1:57-62. (In Russian)
20. Pelevina II, Oradovskaia IV, Mansurova IuG, Aleshchenko AV, Antoshchina MM, Kudriashova OV, Lizunova E Iu, Nikonova MF, Osipov AN, Riabchenko N I, Feoktistov VV, Serebrianyi A M (2010) Communication molecular cell performance and immune status of blood lymphocytes accident liquidators of the Chernobyl. Radiation Biology. Radioecology [Sviaz' molekuliarno-kletochnykh pokazatelei i immunnogo statusa u limfotsitov krovi likvidatorov avarii na ChAES. Radiatsionnaia biologiia. Radioekologiia] 5:501-507. (In Russian)
21. Serebrianyi AM, Antoshchina MM, Aleshchenko AV, Kudriashova OV, Nikonova MF, Riabchenko NI, Iarilin AA (2013) Cytogenetic and immunological parameters stimulated human peripheral blood lymphocytes are associated with cell proliferation rate. Radiation Biology. Radioecology [Tsitogeneticheskie i immunologicheskie pokazateli stimulirovannykh limfotsitov perifericheskoi krovi cheloveka sviazany so skorost'iu proliferatsii kletok. Radiatsionnaia biologiia. Radioekologiia] 6:549-555. (In Russian)
22. Osipov AN, Lizunova EIu, Vorob'eva NIu, Pelevina II (2009) Induction and repair of double-strand DNA breaks in human lymphocytes irradiated in a dose of adapting. Radiation Biology. Radioecology [Induktsiia i reparatsiia dvunitevykh razryvov DNK v limfotsitakh krovi cheloveka, obluchennykh v adaptiruiushchei doze. Radiatsionnaia biologiia. Radioekologiia] 1:42-45. (In Russian)
23. Mikhailov BF, Mazurik VK, Ushenkova LN, Nadezhina NM, Galstian .A, Raeva NF (2006) The study of molecular manifestations of genomic instability in persons exposed to ionizing radiation at clinically relevant doses. Radiation Biology. Radioecology [Issledovanie molekuliarnykh proiavlenii nestabil'nosti genoma u lits, podvergavshikhsia vozdeistviiu ioniziruiushchei radiatsii v klinicheski znachimykh dozakh. Radiatsionnaia biologiia. Radioekologiia] 3:322-336. (In Russian)
24. Akleev AV, Aleshchenko AV, Kudriashova OV, Semenova LP, Serebrianyi AM, Khudiakova OI, Pelevina II (2011) The adaptive response of blood lymphocytes as an indicator of the state of hematopoiesis in irradiated individuals. Radiation Biology. Radioecology [Адаптивный ответ лимфоцитов крови как индикатор состояния гемопоэза у облученных лиц. Radiatsionnaia biologiia. Radioekologiia] 6:645-650. (In Russian)
25. Khandogina EK, Mutovin GR, Zvereva SV, Antipov AV (1991) Adaptive response in irradiated human lymphocytes: radiobiological and genetic aspects, Mutat.Res., 251:181-186. DOI:10.1016/0027-5107(91)90073-W
Загрузки
Как цитировать
Cherednichenko, O. G. (2016). Комбинированное воздействие рентгеновского и γ- излучений на клетки периферической крови человека in vivo и in vitro. Вестник КазНУ. Серия биологическая, 66(1), 106–117. извлечено от https://bb.kaznu.kz/index.php/biology/article/view/1166
Выпуск
Раздел
ФИЗИОЛОГИЯ ЧЕЛОВЕКА И ЖИВОТНЫХ
