Development of an enzyme immunoassay test system for the comprehensive diagnosis of the most pathogenic potato viruses

Abstract

On the basis of the received immune globulin and conjugations an integrated diagnostic test-system was created which is able to detect PVX, PVY, PVS, PVМ, PLRV, PVA viruses of potato at the same time. Optimal characteristics of IFA arrangement were developed and chosen. Optimal characteristics of antigens potato viruses PVМ, PVS, PVY dissolving in sampling conjugation buffer in correlation 1:40 were established. Optimal incubation of virus antigens during the experiment was at the temperature +370С for 2 hours in “sandwich” IFA version.